Detailed information    

experimental Experimentally validated

Overview

Name   mecA   Type   Regulator
Locus tag   STU_RS10485 Genome accession   NC_006448
Coordinates   174410..175159 (+) Length   249 a.a.
NCBI ID   WP_011225365.1    Uniprot ID   Q5M699
Organism   Streptococcus thermophilus LMG 18311     
Function   degradation of ComX   
Competence regulation

Function

σX degradation is performed by the MecA–ClpCP complex.


Genomic Context

Location: 169410..180159
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  STU_RS20080 (stu0156) - 169983..170153 (+) 171 WP_011225360.1 hypothetical protein -
  STU_RS21050 (stu0157) - 170240..170347 (+) 108 WP_011225361.1 LPXTG cell wall anchor domain-containing protein -
  STU_RS10470 (stu0158) - 170436..171176 (-) 741 WP_002949253.1 amino acid ABC transporter ATP-binding protein -
  STU_RS10475 (stu0159) - 171176..172726 (-) 1551 WP_011225362.1 ABC transporter substrate-binding protein/permease -
  STU_RS10480 (stu0160) - 172929..173783 (+) 855 WP_041828107.1 undecaprenyl-diphosphate phosphatase -
  STU_RS19060 (stu0161) - 173929..174144 (-) 216 WP_011225364.1 DUF3021 family protein -
  STU_RS10485 (stu0162) mecA 174410..175159 (+) 750 WP_011225365.1 adaptor protein MecA Regulator
  STU_RS10490 (stu0163) - 175163..176320 (+) 1158 WP_002947632.1 glycosyltransferase family 4 protein -
  STU_RS10495 (stu0164) sufC 176481..177251 (+) 771 WP_002947634.1 Fe-S cluster assembly ATPase SufC -
  STU_RS10500 (stu0165) sufD 177288..178550 (+) 1263 WP_002947640.1 Fe-S cluster assembly protein SufD -
  STU_RS10505 (stu0166) - 178604..179836 (+) 1233 WP_011225367.1 cysteine desulfurase -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  mecA comX negative effect
  comX ssbA positive effect
  comX comGE positive effect
  comX comGB positive effect
  comX comGD positive effect
  comX comGF positive effect
  comX comGG positive effect
  comX radA positive effect
  comX comEC positive effect
  comX comFC positive effect
  comX recA positive effect
  comX comEA positive effect
  comX comGC positive effect
  comX cinA positive effect
  comX coiA positive effect
  comX comGA positive effect
  comX comFA positive effect
  comX dprA positive effect
  clpC comX negative effect
  comR comX positive effect
  comR comS positive effect
  comS comX positive effect
  comS comS positive effect
  amiC comS positive effect
  eeP comS positive effect
  amiD comS positive effect
  amiF comS positive effect
  amiA3 comS positive effect
  amiE comS positive effect
  clpP comX negative effect

Sequence

Protein


Download         Length: 249 a.a.        Molecular weight: 29157.70 Da        Isoelectric Point: 4.0659

>NTDB_id=331 STU_RS10485 WP_011225365.1 174410..175159(+) (mecA) [Streptococcus thermophilus LMG 18311]
MEMKQINETTLKIMITMEDLEEHGMELKDFLIPQEKTEEFFYTVMDELDLPDNFKNSGMLSFRVTPRKDRVDVFVTKSDL
KEALDFNDLSDMEDYSGLSPEEFLKALEDNFMDKGDIEAHHKLEEHDKTLKEVDETMTEPAKEVAEETIREDYTHYVLAF
SDFDQVVTFAQGLKNVSVEGSEFYKLGDVYYMTILLYLADEPDYYANNMYARFLEYANVADRTRPYLQEHATILMEEDAL
PVLQATKWS

Nucleotide


Download         Length: 750 bp        

>NTDB_id=331 STU_RS10485 WP_011225365.1 174410..175159(+) (mecA) [Streptococcus thermophilus LMG 18311]
ATGGAAATGAAACAAATAAACGAGACAACTCTAAAAATCATGATTACCATGGAAGATCTTGAAGAACATGGTATGGAGCT
GAAAGATTTCTTGATTCCCCAGGAAAAAACAGAGGAATTTTTCTATACTGTCATGGATGAGTTGGACTTGCCTGATAATT
TTAAAAATAGTGGGATGTTGAGCTTCCGTGTCACGCCACGAAAGGATCGTGTGGATGTTTTTGTGACTAAATCAGATCTT
AAGGAAGCCCTTGATTTCAATGATTTGTCAGATATGGAAGACTATTCAGGACTTTCTCCAGAAGAATTCCTCAAAGCTCT
TGAAGATAACTTTATGGATAAGGGAGATATTGAAGCCCACCATAAGCTCGAAGAGCATGATAAGACCTTGAAAGAGGTTG
ATGAGACTATGACAGAGCCGGCTAAGGAAGTGGCTGAGGAAACTATTCGTGAAGATTATACTCACTATGTCTTGGCTTTC
TCTGACTTTGATCAAGTAGTGACCTTTGCGCAAGGTTTGAAGAATGTGTCAGTGGAGGGTTCTGAATTTTATAAACTTGG
TGATGTCTACTATATGACTATCCTTCTTTACTTGGCTGATGAGCCTGATTATTACGCGAACAATATGTATGCGCGTTTTC
TTGAATATGCGAATGTGGCAGACCGAACACGTCCCTATCTACAAGAGCATGCAACAATCTTGATGGAAGAGGACGCCTTA
CCGGTATTACAAGCAACGAAATGGAGCTAG

Domains

Predicted by InterproScan.

Negative regulator of genetic competence (MecA) (MecA)

(1-242)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5M699

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  mecA Streptococcus thermophilus LMD-9

99.197

100

0.992
  mecA Streptococcus mutans UA159

57.377

100

0.583
  mecA Streptococcus pneumoniae TIGR4

48.193

100

0.49
  mecA Streptococcus pneumoniae D39

47.791

100

0.486
  mecA Streptococcus pneumoniae R6

47.791

100

0.486
  mecA Streptococcus pneumoniae Rx1

47.791

100

0.486

Multiple sequence alignment    



References

[1] Astrid Wahl et al. (2014) Control of natural transformation in salivarius Streptococci through specific degradation of σX by the MecA-ClpCP protease complex. Journal of Bacteriology 196(15):2807-16. [PMID: 24837292]
[2] Céline Boutry et al. (2012) Adaptor protein MecA is a negative regulator of the expression of late competence genes in Streptococcus thermophilus. Journal of Bacteriology 194(7):1777-88. [PMID: 22287513]