Detailed information NTG0000333

Bioinformatically predicted

Overview

• Name: comEC
• Type: Machinery gene
• Locus tag: STU_RS16670
• Genome accession: NC_006448
• Coordinates: 1378489..1380729 (-)
• Length: 746 a.a.
• NCBI ID: WP_011226399.1
• Uniprot ID: Q5M375
• Organism: Streptococcus thermophilus LMG 18311
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1373489..1385729

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
STU_RS16650 (stu1557)	-	1374353..1375213 (-)	861	WP_011226395.1	methionyl aminopeptidase	-
STU_RS16655 (stu1558)	spxR	1375257..1376537 (-)	1281	WP_011227467.1	CBS-HotDog domain-containing transcription factor SpxR	-
STU_RS16660 (stu1559)	-	1376530..1377075 (-)	546	WP_041828309.1	GNAT family N-acetyltransferase	-
STU_RS16665 (stu1560)	-	1377141..1378403 (-)	1263	WP_022096991.1	UDP-N-acetylglucosamine 1-carboxyvinyltransferase	-
STU_RS16670 (stu1561)	comEC	1378489..1380729 (-)	2241	WP_011226399.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
STU_RS16675 (stu1562)	comEA	1380719..1381414 (-)	696	WP_011226400.1	helix-hairpin-helix domain-containing protein	Machinery gene
STU_RS16680 (stu1563)	-	1381522..1382277 (-)	756	WP_002948862.1	lysophospholipid acyltransferase family protein	-
STU_RS21155 (stu1564)	-	1382477..1383010 (+)	534	WP_011226401.1	polysaccharide deacetylase family protein	-
STU_RS20725 (stu1565)	-	1382944..1383342 (+)	399	WP_288576965.1	peptidoglycan GlcNAc deacetylase	-
STU_RS16695 (stu1566)	-	1383392..1384156 (+)	765	WP_011226403.1	tRNA1(Val) (adenine(37)-N6)-methyltransferase	-
STU_RS16700 (stu1567)	-	1384160..1384429 (+)	270	WP_011226404.1	GIY-YIG nuclease family protein	-

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
comX	comEC	positive effect
comX	ssbA	positive effect
comX	comGE	positive effect
comX	comGB	positive effect
comX	comGD	positive effect
comX	comGF	positive effect
comX	comGG	positive effect
comX	radA	positive effect
comX	comFC	positive effect
comX	recA	positive effect
comX	comEA	positive effect
comX	comGC	positive effect
comX	cinA	positive effect
comX	coiA	positive effect
comX	comGA	positive effect
comX	comFA	positive effect
comX	dprA	positive effect
clpC	comX	negative effect
comR	comX	positive effect
comR	comS	positive effect
comS	comX	positive effect
comS	comS	positive effect
amiC	comS	positive effect
eeP	comS	positive effect
amiD	comS	positive effect
amiF	comS	positive effect
amiA3	comS	positive effect
amiE	comS	positive effect
clpP	comX	negative effect
mecA	comX	negative effect

Sequence

Protein

• Length: 746 a.a.
• Molecular weight: 84884.89 Da
• Isoelectric Point: 9.9608

>NTDB_id=333 STU_RS16670 WP_011226399.1 1378489..1380729(-) (comEC) [Streptococcus thermophilus LMG 18311]
MWLKKAPINLFSLALLIVALYFTIFVSNFYAIGTFVFLMICFLRHHWKNRAALKFVGLIGGFFLVYFLFLYHIAIMQDKQ
TPAEIHQVTLVADTLSVNGERLSAIGKSNGQTYQVFYRLKSDKEQHFFKTTSQTLVLKGKIKLSSATGQRNFQGFDYQSY
LASQGIYRIAQIDRLEHVVIPKSISPIAFFHQLRRKALVHIQTHFPNPMRHYMTGLLFGYLDKEFDEQSQLYTSLGIIHL
FALSGMQVGFFLGWFRYGLLRLGLPKNYILAILLPFSIFYGLMTGWTASVLRSLIQSLLAECGIKKLNNMGITLLLFFLV
LPHFLLTVGGVLSCSYAFLLCLFNFEEMPSFKKSIYMSLVLSLGTLPFLTYYYGTFQPLSLILTAIFSLVFDSFLLPVLT
VLFTLSGMVIFSQFNTLFEWMEFFLTWVQSWGVQPLILGKPSLFQFVSMIFVLVLLFDFWKKPQFRISLLMIFSLLMVWV
KHPLINEVTVVDVGQGDSIFLRSMKGETVLIDVGGKVTFGSKEKWQEGSQTSNAEKTLIPYLQARGVSQIDYLVLTHTDT
DHIGDLEVVAKRFKIKKICVSEGALTKPSFAKRIRFLKCPVRTLKAGDKLPMMGSNLQVLYPNKIGNGGNNDSLVLYGKL
LGTSFLFTGDLEKEGEEELMTSYPNLKVRVLKAGHHGSKGSSSEAFLDQLKPSLALVSAGENNRYKHPNDETLERFKERH
IKVLRTDQDGAIRFKGWFQLSSETVR

Nucleotide

• Length: 2241 bp

>NTDB_id=333 STU_RS16670 WP_011226399.1 1378489..1380729(-) (comEC) [Streptococcus thermophilus LMG 18311]
ATGTGGCTTAAAAAAGCTCCAATCAATCTTTTTTCCCTAGCGCTTTTAATAGTTGCTCTTTATTTTACTATTTTTGTATC
TAATTTCTATGCTATTGGGACTTTTGTCTTTCTGATGATATGTTTTTTGAGGCATCATTGGAAGAATAGAGCAGCTCTAA
AGTTTGTGGGACTTATAGGTGGTTTCTTTTTGGTCTACTTTTTATTTTTATACCATATAGCTATCATGCAAGATAAACAA
ACTCCTGCTGAAATTCATCAGGTGACACTGGTTGCAGATACGCTCTCAGTTAATGGTGAGCGATTATCAGCAATCGGGAA
GTCAAATGGACAAACCTATCAGGTCTTTTACCGGCTAAAATCTGATAAGGAGCAGCATTTTTTTAAGACTACTAGTCAAA
CGCTTGTTTTAAAAGGGAAAATAAAGTTGTCCTCGGCAACTGGTCAACGTAATTTTCAAGGATTTGATTATCAGTCTTAT
CTAGCTAGTCAGGGCATTTATAGGATTGCTCAGATTGACCGTCTGGAACATGTCGTAATCCCAAAATCGATATCTCCAAT
AGCTTTTTTTCATCAATTGAGGAGGAAGGCTCTAGTTCATATTCAGACGCATTTTCCTAATCCGATGAGACACTACATGA
CAGGACTGCTCTTTGGGTATTTGGACAAGGAGTTTGATGAGCAGAGTCAACTCTACACTAGCTTGGGGATTATTCATCTT
TTTGCGCTATCGGGTATGCAGGTTGGATTTTTTCTTGGCTGGTTTCGCTATGGACTTCTCCGTTTGGGGCTTCCCAAAAA
TTATATCCTTGCTATCTTATTACCTTTTTCGATTTTCTATGGCTTAATGACTGGTTGGACGGCTTCGGTCTTACGTTCTT
TGATTCAAAGCCTCTTGGCTGAGTGTGGTATTAAAAAACTGAACAATATGGGCATAACGCTACTTCTATTTTTTCTAGTC
TTGCCTCATTTTCTTTTAACGGTAGGAGGTGTTTTAAGTTGTTCTTATGCCTTCTTGTTGTGTTTATTTAATTTTGAGGA
GATGCCGTCCTTTAAAAAGTCAATTTATATGAGTTTAGTATTGAGTCTTGGGACTTTGCCTTTTTTGACCTACTATTATG
GAACTTTTCAACCATTGAGTTTGATTCTGACGGCAATCTTCTCTCTAGTTTTTGATAGCTTTCTCTTACCTGTCTTAACA
GTACTTTTTACACTTTCAGGAATGGTAATTTTTTCTCAATTTAACACACTTTTTGAATGGATGGAGTTCTTTTTGACTTG
GGTACAATCTTGGGGAGTCCAGCCATTGATTTTAGGAAAACCTAGCTTGTTTCAGTTTGTCTCAATGATATTTGTATTGG
TTTTGCTCTTTGATTTTTGGAAAAAGCCTCAGTTTAGAATATCCCTCTTGATGATTTTTAGTCTTTTGATGGTCTGGGTC
AAACATCCTTTAATAAATGAAGTAACGGTGGTCGACGTTGGTCAAGGAGATAGTATTTTTTTAAGGAGTATGAAAGGTGA
AACGGTCCTAATAGATGTAGGTGGCAAGGTGACTTTCGGCTCTAAGGAAAAATGGCAAGAGGGGAGCCAGACGAGTAATG
CGGAGAAAACCTTAATTCCCTATCTACAGGCAAGGGGAGTGTCTCAAATTGACTATCTGGTTCTGACTCATACGGATACA
GACCATATTGGTGATTTGGAGGTAGTGGCTAAACGGTTTAAGATTAAGAAAATTTGTGTCAGTGAGGGGGCTTTGACTAA
GCCTAGTTTTGCCAAACGTATTCGATTTCTTAAATGTCCCGTTCGCACTTTAAAGGCTGGTGATAAGCTGCCTATGATGG
GAAGTAACCTACAGGTTCTTTATCCCAATAAAATTGGTAATGGTGGTAACAATGATTCACTTGTTCTCTATGGAAAGTTA
TTGGGAACCAGTTTTCTATTTACTGGTGATTTGGAAAAAGAAGGAGAAGAGGAATTGATGACTAGCTATCCAAATTTAAA
AGTAAGGGTCCTCAAAGCAGGACATCACGGTTCTAAAGGCTCTTCATCAGAAGCTTTTTTGGATCAGCTAAAGCCATCAC
TTGCTCTTGTCTCAGCAGGTGAAAATAATCGTTATAAGCATCCAAATGATGAAACATTAGAGCGTTTCAAAGAACGTCAC
ATTAAGGTTTTACGCACAGACCAGGATGGTGCCATTCGGTTTAAGGGATGGTTCCAACTATCAAGTGAAACTGTCCGATA
A

Domains

Predicted by InterproScan.

• Metallo-beta-lactamase superfamily (Lactamase_B) (492-699)

• Competence protein (Competence) (216-462)

• Domain of unknown function (DUF4131) (DUF4131) (29-170)

3D structure

• AlphaFold DB: Q5M375

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEC/celB	Streptococcus mitis NCTC 12261	47.587	100	0.476
comEC/celB	Streptococcus mitis SK321	46.72	100	0.468
comEC/celB	Streptococcus pneumoniae TIGR4	45.783	100	0.458
comEC/celB	Streptococcus pneumoniae D39	45.515	100	0.456
comEC/celB	Streptococcus pneumoniae Rx1	45.515	100	0.456
comEC/celB	Streptococcus pneumoniae R6	45.515	100	0.456
comEC	Lactococcus lactis subsp. cremoris KW2	45.379	97.185	0.441