Detailed information    

experimental Experimentally validated

Overview

Name   eeP   Type   Regulator
Locus tag   STU_RS10650 Genome accession   NC_006448
Coordinates   204592..205854 (+) Length   420 a.a.
NCBI ID   WP_011225396.1    Uniprot ID   Q5M675
Organism   Streptococcus thermophilus LMG 18311     
Function   processing of ComS   
Competence regulation

Function

At the cell surface, the protease Eep processes ComS, releasing XIP into the external milieu.


Genomic Context

Location: 199592..210854
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  STU_RS10625 (stu0194) - 200173..201522 (+) 1350 WP_011680683.1 glucose-6-phosphate isomerase -
  STU_RS10630 (stu0195) - 201710..202426 (+) 717 WP_002949309.1 YebC/PmpR family DNA-binding transcriptional regulator -
  STU_RS10635 (stu0196) - 202552..202890 (+) 339 WP_011225395.1 preprotein translocase subunit YajC -
  STU_RS10640 (stu0197) - 203019..203768 (+) 750 WP_002949314.1 isoprenyl transferase -
  STU_RS10645 (stu0198) - 203781..204575 (+) 795 WP_002949317.1 phosphatidate cytidylyltransferase -
  STU_RS10650 (stu0199) eeP 204592..205854 (+) 1263 WP_011225396.1 RIP metalloprotease RseP Regulator
  STU_RS10655 (stu0200) - 205925..207787 (+) 1863 WP_011225397.1 proline--tRNA ligase -
  STU_RS10660 (stu0201) - 208404..210344 (+) 1941 WP_041828121.1 ABC transporter substrate binding protein -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  eeP comS positive effect
  comS comS positive effect
  comS comX positive effect
  amiC comS positive effect
  amiD comS positive effect
  comR comS positive effect
  amiF comS positive effect
  amiA3 comS positive effect
  amiE comS positive effect
  comX ssbA positive effect
  comX comGE positive effect
  comX comGB positive effect
  comX comGD positive effect
  comX comGF positive effect
  comX comGG positive effect
  comX radA positive effect
  comX comEC positive effect
  comX comFC positive effect
  comX recA positive effect
  comX comEA positive effect
  comX comGC positive effect
  comX cinA positive effect
  comX coiA positive effect
  comX comGA positive effect
  comX comFA positive effect
  comX dprA positive effect
  clpC comX negative effect
  comR comX positive effect
  clpP comX negative effect
  mecA comX negative effect

Sequence

Protein


Download         Length: 420 a.a.        Molecular weight: 45620.10 Da        Isoelectric Point: 7.3561

>NTDB_id=323 STU_RS10650 WP_011225396.1 204592..205854(+) (eeP) [Streptococcus thermophilus LMG 18311]
MKAIITFLLIFCVIVVFHEFGHFFFAKRSGILVREFAIGMGPKIFAHTGKDGTVYTIRILPLGGYVRMAGWGEDTTEIKT
GSPASLTLGKDGKVRRINLSDRQVDQTALPMNVTAYDLEDKLTITGLVLGETKTYEVDHDATIVEEDGTELRIAPKDVQY
QNASIWGRLITNFAGPMNNFILGVLVFIILAFVQGGVQDTSTNLIQVANGGAAQVSGLKTGDAIVAINKDKVTDWDSLKE
ALRENTQKFSKGDSLSVTVKRSNGQEETISVKPQKSQGSYFLGVSPVLKTGLKDKIFGGFQMAWEGATAILATLKGLITN
FSLNKLGGPVAMFQMSAQASESGLISILDLMGMLSINLGIFNLIPIPALDGGKIVMNIIEAIRRKPLNQEIESYITLAGV
AVMVVLMIAVTWNDIMRAFF

Nucleotide


Download         Length: 1263 bp        

>NTDB_id=323 STU_RS10650 WP_011225396.1 204592..205854(+) (eeP) [Streptococcus thermophilus LMG 18311]
ATGAAAGCTATTATTACTTTTTTACTGATTTTCTGTGTGATTGTCGTCTTCCATGAGTTTGGCCATTTCTTCTTTGCCAA
ACGATCAGGCATTCTGGTGCGTGAATTTGCCATTGGTATGGGTCCTAAAATTTTTGCACACACTGGTAAGGATGGTACCG
TTTATACTATTCGTATCCTTCCTTTGGGTGGTTATGTGCGTATGGCTGGTTGGGGTGAAGATACAACTGAGATTAAAACG
GGGAGTCCTGCTAGCCTAACGCTTGGAAAAGATGGCAAAGTGAGACGTATCAATCTTTCAGATCGTCAGGTTGACCAGAC
GGCTTTGCCTATGAACGTTACTGCCTATGATTTGGAAGATAAGTTAACTATTACAGGTCTTGTCCTAGGTGAGACCAAGA
CCTATGAGGTTGACCATGATGCCACTATCGTTGAGGAAGACGGTACGGAGCTTCGTATTGCTCCTAAGGATGTCCAATAT
CAAAATGCTAGCATTTGGGGACGTTTAATTACCAATTTCGCTGGTCCTATGAACAACTTTATTCTTGGGGTCTTGGTCTT
TATTATCCTTGCCTTTGTTCAAGGTGGTGTTCAAGATACGTCGACCAACCTTATCCAGGTGGCTAATGGTGGTGCTGCCC
AGGTGTCAGGTTTGAAAACTGGTGATGCCATCGTGGCCATTAACAAGGACAAGGTTACTGACTGGGACAGCTTGAAAGAA
GCATTGAGAGAGAATACCCAAAAATTCTCGAAAGGCGACAGCCTTTCTGTGACGGTTAAGAGAAGTAATGGTCAGGAAGA
AACTATTTCTGTTAAACCCCAAAAGAGCCAAGGGAGCTACTTTTTAGGTGTCTCTCCTGTCTTAAAGACAGGTCTTAAAG
ATAAGATTTTTGGTGGTTTCCAAATGGCTTGGGAAGGTGCGACGGCAATCCTCGCTACGCTCAAAGGGCTCATTACCAAC
TTTAGCTTGAACAAGCTTGGTGGACCGGTTGCTATGTTCCAGATGTCTGCACAAGCCTCAGAAAGTGGGTTGATTTCCAT
TCTTGACCTTATGGGAATGCTCTCTATCAACTTAGGGATTTTCAATCTTATTCCAATTCCAGCCCTTGACGGTGGTAAAA
TTGTTATGAATATTATTGAAGCTATTCGTCGTAAACCACTTAATCAAGAAATTGAAAGCTATATCACACTCGCAGGTGTT
GCGGTCATGGTTGTCCTTATGATTGCAGTAACTTGGAATGATATTATGCGTGCTTTCTTTTAA

Domains

Predicted by InterproScan.

Peptidase family M50 (Peptidase_M50)

(6-406)

PDZ domain (PDZ_2)

(204-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5M675

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  eeP Streptococcus thermophilus LMD-9

99.762

100

0.998

Multiple sequence alignment    



References

[1] Rozenn Gardan et al. (2013) Extracellular life cycle of ComS, the competence-stimulating peptide of Streptococcus thermophilus. Journal of Bacteriology 195(8):1845-55. [PMID: 23396911]