Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   STU_RS16675 Genome accession   NC_006448
Coordinates   1380719..1381414 (-) Length   231 a.a.
NCBI ID   WP_011226400.1    Uniprot ID   Q5M374
Organism   Streptococcus thermophilus LMG 18311     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1375719..1386414
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  STU_RS16660 (stu1559) - 1376530..1377075 (-) 546 WP_041828309.1 GNAT family N-acetyltransferase -
  STU_RS16665 (stu1560) - 1377141..1378403 (-) 1263 WP_022096991.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
  STU_RS16670 (stu1561) comEC 1378489..1380729 (-) 2241 WP_011226399.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  STU_RS16675 (stu1562) comEA 1380719..1381414 (-) 696 WP_011226400.1 helix-hairpin-helix domain-containing protein Machinery gene
  STU_RS16680 (stu1563) - 1381522..1382277 (-) 756 WP_002948862.1 lysophospholipid acyltransferase family protein -
  STU_RS21155 (stu1564) - 1382477..1383010 (+) 534 WP_011226401.1 polysaccharide deacetylase family protein -
  STU_RS20725 (stu1565) - 1382944..1383342 (+) 399 WP_288576965.1 peptidoglycan GlcNAc deacetylase -
  STU_RS16695 (stu1566) - 1383392..1384156 (+) 765 WP_011226403.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  STU_RS16700 (stu1567) - 1384160..1384429 (+) 270 WP_011226404.1 GIY-YIG nuclease family protein -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  comX comEA positive effect
  comX ssbA positive effect
  comX comGE positive effect
  comX comGB positive effect
  comX comGD positive effect
  comX comGF positive effect
  comX comGG positive effect
  comX radA positive effect
  comX comEC positive effect
  comX comFC positive effect
  comX recA positive effect
  comX comGC positive effect
  comX cinA positive effect
  comX coiA positive effect
  comX comGA positive effect
  comX comFA positive effect
  comX dprA positive effect
  clpC comX negative effect
  comR comX positive effect
  comR comS positive effect
  comS comX positive effect
  comS comS positive effect
  amiC comS positive effect
  eeP comS positive effect
  amiD comS positive effect
  amiF comS positive effect
  amiA3 comS positive effect
  amiE comS positive effect
  clpP comX negative effect
  mecA comX negative effect

Sequence

Protein


Download         Length: 231 a.a.        Molecular weight: 24466.40 Da        Isoelectric Point: 4.6300

>NTDB_id=332 STU_RS16675 WP_011226400.1 1380719..1381414(-) (comEA) [Streptococcus thermophilus LMG 18311]
MKEKILAYVKDNRLFVSVIAVLMVIFCFFLWMTCGAGNSMEAETSYTDVTALSTSSSKQSSQSLSEASSQSKTEGSEKVK
SKVTVDVKGAVVNPGVYTLKAGARVTDVIQEAGGITEDADAKSVNLAASLSDEEVIYVANKDENVSVLDQTGTGQVSDKG
GQAVSKDGKINLNTATSEQLQTISGIGAKRAEDIIAYRESHGGFQSVDDLKNVSGIGDKTLDKIRESLYVA

Nucleotide


Download         Length: 696 bp        

>NTDB_id=332 STU_RS16675 WP_011226400.1 1380719..1381414(-) (comEA) [Streptococcus thermophilus LMG 18311]
GTGAAGGAAAAGATTCTAGCCTATGTCAAAGATAATCGTCTGTTTGTGAGTGTCATCGCTGTACTGATGGTGATTTTTTG
CTTCTTTTTATGGATGACTTGTGGTGCCGGCAACAGCATGGAGGCAGAGACGTCTTATACAGATGTGACAGCTTTGTCAA
CGTCTTCATCCAAACAAAGTTCACAGTCTCTTTCTGAGGCGTCTTCCCAGTCAAAAACCGAAGGAAGTGAAAAAGTTAAG
TCAAAAGTAACGGTAGATGTTAAGGGGGCTGTGGTCAATCCAGGTGTCTATACGCTAAAGGCAGGCGCTAGGGTGACAGA
TGTCATTCAGGAAGCTGGAGGAATTACAGAAGATGCAGACGCTAAGAGTGTTAACTTAGCCGCAAGCTTGTCAGATGAAG
AGGTTATTTATGTAGCCAATAAAGATGAAAATGTTTCTGTCCTTGATCAAACAGGTACTGGTCAGGTCTCTGACAAAGGG
GGGCAGGCTGTATCTAAGGATGGCAAAATTAACTTAAATACAGCGACCTCAGAACAGTTGCAAACCATTTCTGGAATTGG
AGCTAAGCGGGCAGAGGATATCATTGCCTATCGTGAAAGTCATGGCGGCTTTCAGTCCGTAGATGACTTGAAAAATGTCT
CAGGAATTGGTGATAAAACATTAGATAAAATCAGAGAGTCCCTCTATGTGGCTTAA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(85-138)

Helix-hairpin-helix motif (HHH_3)

(167-228)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5M374

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Streptococcus thermophilus LMD-9

99.134

100

0.991
  comEA/celA/cilE Streptococcus mitis SK321

41.048

99.134

0.407
  comEA/celA/cilE Streptococcus pneumoniae D39

39.474

98.701

0.39
  comEA/celA/cilE Streptococcus pneumoniae R6

39.474

98.701

0.39
  comEA/celA/cilE Streptococcus pneumoniae Rx1

39.474

98.701

0.39
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

39.474

98.701

0.39
  comEA/celA/cilE Streptococcus mitis NCTC 12261

38.158

98.701

0.377

Multiple sequence alignment