Detailed information    

experimental Experimentally validated

Overview

Name   comEA/celA/cilE   Type   Machinery gene
Locus tag   SP_RS04730 Genome accession   NC_003028
Coordinates   901982..902632 (+) Length   216 a.a.
NCBI ID   WP_000387344.1    Uniprot ID   A0A0T9GI09
Organism   Streptococcus pneumoniae TIGR4     
Function   dsDNA binding to the cell surface   
DNA binding and uptake

Function

ComEA is a DNA-binding protein critical for the uptake of extracellular DNA during natural transformation. It binds to double-stranded DNA (dsDNA) at the cell surface, facilitating its transport into the cell through the competence-specific DNA uptake machinery. ComEA works in concert with other proteins such as ComEC, which is involved in the transport of single-stranded DNA into the cell.


Genomic Context

Location: 896982..907632
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SP_RS12760 (SP_0949) - 897942..898756 (+) 815 Protein_893 IS5-like element IS1515 family transposase -
  SP_RS04705 (SP_0950) - 898939..899439 (+) 501 WP_000566988.1 GNAT family N-acetyltransferase -
  SP_RS04710 (SP_0951) - 899442..899768 (+) 327 Protein_895 TfoX/Sxy family protein -
  SP_RS12055 (SP_0952) ald 900069..901168 (-) 1100 Protein_896 alanine dehydrogenase -
  SP_RS04725 (SP_0953) - 901345..901914 (+) 570 WP_000443750.1 GNAT family N-acetyltransferase -
  SP_RS04730 (SP_0954) comEA/celA/cilE 901982..902632 (+) 651 WP_000387344.1 helix-hairpin-helix domain-containing protein Machinery gene
  SP_RS04735 (SP_0955) comEC/celB 902616..904856 (+) 2241 WP_000942394.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  SP_RS04740 (SP_0956) - 905035..905223 (+) 189 WP_001808514.1 hypothetical protein -
  SP_RS04745 (SP_0957) - 905256..905843 (+) 588 WP_000939895.1 ATP-binding cassette domain-containing protein -
  SP_RS04750 (SP_0958) - 905847..907031 (+) 1185 WP_000655960.1 ABC transporter permease -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  htrA comEA/celA/cilE negative effect
  htrA comC/comC2 negative effect
  comC/comC2 comD/comD2 positive effect
  comD/comD2 comE positive effect
  comE comE positive effect
  comE comX/comX1 positive effect
  comE comW positive effect
  comE comA positive effect
  comE comB positive effect
  comE comM positive effect
  comE comX/comX2 positive effect
  comE comD/comD2 positive effect
  comE comC/comC2 positive effect
  stkP comE positive effect
  comX/comX1 late competence genes positive effect
  clpP comX/comX1 negative effect
  comW comX/comX1 positive effect
  clpE comX/comX1 negative effect
  comW comX/comX2 positive effect
  clpP comW negative effect
  mecA comW negative effect
  clpC comW negative effect
  comA comC/comC2 positive effect
  comB comC/comC2 positive effect
  comM cbpD negative effect
  comX/comX2 late competence genes positive effect
  clpP comX/comX2 negative effect
  clpE comX/comX2 negative effect
  ciaH comC/comC2 negative effect
  ciaR comC/comC2 negative effect
  comX/comX2 late competence genes positive effect
  comX/comX1 late competence genes positive effect
  cbpD lytA positive effect
  cbpD lytC positive effect
  ciaH htrA positive effect
  htrA comEC/celB negative effect
  ciaR htrA positive effect

Sequence

Protein


Download         Length: 216 a.a.        Molecular weight: 23181.48 Da        Isoelectric Point: 6.0746

>NTDB_id=153 SP_RS04730 WP_000387344.1 901982..902632(+) (comEA/celA/cilE) [Streptococcus pneumoniae TIGR4]
MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQAEVAAVSKDSSTEKEVKKEEKEEPLEQDLITVDVKG
AVKSPGIYDLPVGSRVNDAVQKAGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSTSKEKKVNLNKA
SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGGKTIEKLKDYVTVD

Nucleotide


Download         Length: 651 bp        

>NTDB_id=153 SP_RS04730 WP_000387344.1 901982..902632(+) (comEA/celA/cilE) [Streptococcus pneumoniae TIGR4]
ATGGAAGCAATTATCGAGAAAATCAAAGAGTATAAAATCATCGTCATCTGTACTGGTCTGGGCTTGCTTGTAGGAGGATT
TTTCCTGCTAAAACCAGCTCCACAAACACCTGTCAAAGAGACGAATTTGCAGGCTGAAGTTGCAGCTGTTTCCAAGGACT
CATCGACCGAAAAGGAAGTGAAGAAGGAAGAAAAGGAAGAACCCCTTGAACAAGATCTAATCACAGTAGATGTCAAAGGT
GCTGTCAAATCGCCAGGGATTTATGACTTGCCTGTAGGTAGTCGAGTCAATGATGCTGTTCAGAAGGCTGGTGGCTTGAC
AGAGCAAGCAGACAGCAAGTCGCTCAATCTAGCTCAGAAAGTTAGTGATGAGGCTCTGGTTTACGTTCCTACTAAGGGAG
AAGAAGCAGTTAGTCAACAGACTGGTTCGGGGACAGCTTCTTCAACAAGCAAGGAAAAGAAGGTCAATCTCAACAAGGCC
AGTCTGGAAGAACTCAAGCAGGTCAAGGGACTGGGAGGAAAACGAGCTCAGGACATTATTGACCATCGTGAGGCAAATGG
CAAGTTCAAGTCAGTAGACGAGCTCAAGAAGGTCTCTGGCATTGGTGGCAAAACAATAGAAAAGCTTAAAGACTATGTTA
CAGTGGATTAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(151-214)

SLBB domain (SLBB)

(76-126)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0T9GI09

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA/celA/cilE Streptococcus pneumoniae R6

97.222

100

0.972
  comEA/celA/cilE Streptococcus pneumoniae D39

97.222

100

0.972
  comEA/celA/cilE Streptococcus pneumoniae Rx1

97.222

100

0.972
  comEA/celA/cilE Streptococcus mitis NCTC 12261

96.759

100

0.968
  comEA/celA/cilE Streptococcus mitis SK321

93.056

100

0.931
  comEA Lactococcus lactis subsp. cremoris KW2

42.291

100

0.44
  Cj0011c Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

49.206

79.747

0.392
  comEA Streptococcus thermophilus LMD-9

39.035

98.701

0.385
  comEA Latilactobacillus sakei subsp. sakei 23K

34.335

100

0.37

Multiple sequence alignment    



References

[1] Yanni Liu et al. (2019) HtrA-mediated selective degradation of DNA uptake apparatus accelerates termination of pneumococcal transformation. Molecular Microbiology 112(4):1308-1325. [PMID: 31396996]
[2] Matthieu J Bergé et al. (2013) Midcell recruitment of the DNA uptake and virulence nuclease, EndA, for pneumococcal transformation. PLoS Pathogens 9(9):e1003596. [PMID: 24039578]
[3] Scott N Peterson et al. (2004) Identification of competence pheromone responsive genes in Streptococcus pneumoniae by use of DNA microarrays. Molecular Microbiology 51(4):1051-70. [PMID: 14763980]
[4] Mathieu Bergé et al. (2002) Uptake of transforming DNA in Gram-positive bacteria: a view from Streptococcus pneumoniae. Molecular Microbiology 45(2):411-21. [PMID: 12123453]
[5] E V Pestova et al. (1998) Isolation and characterization of three Streptococcus pneumoniae transformation-specific loci by use of a lacZ reporter insertion vector. Journal of Bacteriology 180(10):2701-10. [PMID: 9573156]