Detailed information    

experimental Experimentally validated

Overview

Name   lytC   Type   Auxiliary factor
Locus tag   SP_RS07755 Genome accession   NC_003028
Coordinates   1479067..1480571 (-) Length   500 a.a.
NCBI ID   Protein_1484    Uniprot ID   -
Organism   Streptococcus pneumoniae TIGR4     
Function   increase the efficiency of the cell lysis   
Cell lysis

Function

The two peptidoglycan-hydrolyzing enzymes LytA and LytC, which are present in non-competent target cells, contribute to enhanced lysis during fratricide. Studies have shown that the muralytic activity of CbpD activates LytA and LytC in target cells, thereby accelerating and increasing the efficiency of the lysis reaction


Genomic Context

Location: 1474067..1485571
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SP_RS07725 (SP_1567) - 1474996..1475376 (-) 381 WP_001140412.1 RidA family protein -
  SP_RS07730 (SP_1568) yihA 1475387..1475974 (-) 588 WP_000422599.1 ribosome biogenesis GTP-binding protein YihA/YsxC -
  SP_RS07735 (SP_1569) clpX 1475983..1477215 (-) 1233 WP_000106346.1 ATP-dependent Clp protease ATP-binding subunit ClpX Regulator
  SP_RS07745 (SP_1571) - 1477417..1477923 (-) 507 WP_000162484.1 dihydrofolate reductase -
  SP_RS07750 (SP_1572) - 1478053..1478571 (-) 519 WP_000229874.1 Dps family protein -
  SP_RS07755 (SP_1573) lytC 1479067..1480571 (-) 1505 Protein_1483 choline binding-anchored murein hydrolase LytC Auxiliary factor
  SP_RS07760 (SP_1574) tpiA 1480609..1481367 (-) 759 WP_000087897.1 triose-phosphate isomerase -
  SP_RS07765 (SP_1575) - 1481465..1482142 (-) 678 WP_000221613.1 DnaD domain-containing protein -
  SP_RS07770 (SP_1576) metA 1482151..1483095 (-) 945 WP_001122709.1 homoserine O-acetyltransferase MetA -
  SP_RS07775 (SP_1577) - 1483277..1483789 (-) 513 WP_001049323.1 adenine phosphoribosyltransferase -
  SP_RS07780 (SP_1578) - 1483876..1484634 (-) 759 WP_001287230.1 class I SAM-dependent methyltransferase -
  SP_RS13055 - 1484842..1484988 (+) 147 WP_000403100.1 hypothetical protein -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  cbpD lytC positive effect
  cbpD lytA positive effect
  comM cbpD negative effect
  comE comM positive effect
  comE comE positive effect
  comE comX/comX1 positive effect
  comE comW positive effect
  comE comA positive effect
  comE comB positive effect
  comE comX/comX2 positive effect
  comE comD/comD2 positive effect
  comE comC/comC2 positive effect
  comD/comD2 comE positive effect
  stkP comE positive effect
  comX/comX1 late competence genes positive effect
  clpP comX/comX1 negative effect
  comW comX/comX1 positive effect
  clpE comX/comX1 negative effect
  comW comX/comX2 positive effect
  clpP comW negative effect
  mecA comW negative effect
  clpC comW negative effect
  comA comC/comC2 positive effect
  comB comC/comC2 positive effect
  comX/comX2 late competence genes positive effect
  clpP comX/comX2 negative effect
  clpE comX/comX2 negative effect
  comC/comC2 comD/comD2 positive effect
  ciaH comC/comC2 negative effect
  htrA comC/comC2 negative effect
  ciaR comC/comC2 negative effect
  comX/comX2 late competence genes positive effect
  comX/comX1 late competence genes positive effect
  ciaH htrA positive effect
  htrA comEC/celB negative effect
  htrA comEA/celA/cilE negative effect
  ciaR htrA positive effect

Sequence

Protein


Download         Length: 500 a.a.        Molecular weight: Da        Isoelectric Point:

>NTDB_id=151 SP_RS07755 Protein_1484 1479067..1480571(-) (lytC) [Streptococcus pneumoniae TIGR4]
None

Nucleotide


Download         Length: 1505 bp        

>NTDB_id=151 SP_RS07755 Protein_1484 1479067..1480571(-) (lytC) [Streptococcus pneumoniae TIGR4]
ATGTCTGTTACATTTTTTATAGGAGAGAAAGATTGAAAACAAAAATTGGATTAGCAAGTATCTGTTTACTAGGCTTGGCA
ACTAGTCATGTCGCTGCAAATGAAACTGAAGTAGCAAAAACTTCGCAGGATACAACGACAGCTTCAAGTAGTTCAGAGCA
AAATCAGTCTTCTAATAAAACGCAAACGAGCGCAGAAGTACAGACTAATGCTGCTGCCCACTGGGATGGGGATTATTATG
TAAAGGATGATGGTTCTAAAGCTCAAAGTGAATGGATTTTTGACAACTACTATAAGGCTTGGTTTTATATTAATTCAGAT
GGTCGTTACTCGCAGAATGAATGGCATGGAAATTACTACCTGAAATCAGGTGGATATATGGCCCAAAACGAGTGGATCTA
TGACAGTAATTACAAGAGTTGGTTTTATCTCAAGTCAGATGGGGCTTATGCTCATCAAGAATGGCAATTGATTGGAAATA
AGTGGTACTACTTCAAGAAGTGGGGTTACATGGCTAAAAGCCAATGGCAAGGAAGTTATTTCTTGAATGGTCAAGGAGCT
ATGATGCAAAATGAATGGCTCTATGATCCAGCCTATTCTGCTTATTTTTATCTAAAATCCGATGGAACTTATGCTAACCA
AGAGTGGCAAAAAGTGGGCGGCAAATGGTACTATTTCAAGAAGTGGGGCTATATGGCTCGGAATGAGTGGCAAGGCAACT
ACTATTTGACTGGAAGTGGTGCCATGGCGACTGACGAAGTGATTATGGATGGTACTCGCTATATCTTTGCGGCCTCTGGT
GAGCTCAAAGAAAAAAAAGATTTGAATGTCGGCTGGGTTCACAGAGATGGTAAGCGCTATTTCTTTAATAATAGAGAAGA
ACAAGTGGGAACCGAACATGCTAAGAAAGTCATTGATATTAGTGAGCACAATGGTCGTATCAATGATTGGAAAAAGGTTA
TTGATGAGAACGAAGTGGATGGTGTCATTGTTCGTCTAGGTTATAGCGGTAAAGAAGACAAGGAATTGGCGCATAACATT
AAGGAGTTAAACCGTCTGGGAATTCCTTATGGTGTCTATCTCTATACCTATGCTGAAAATGAGACCGATGCTGAGAGTGA
CGCTAAACAGACCATTGAACTTATAAAGAAATACAATATGAACCTGTCTTACCCTATCTATTATGATGTTGAGAATTGGG
AATATGTAAATAAGAGCAAGAGAGCTCCAAGTGATACAGGCACTTGGGTTAAAATCATCAACAAGTACATGGACACGATG
AAGCAGGCGGGTTATCAAAATGTGTATGTCTATAGCTATCGTAGTTTATTACAGACGCGTTTAAAACACCCAGATATTTT
AAAACATGTAAACTGGGTAGCGGCCTATACGAATGCTTTAGAATGGGAAAACCCTCATTATTCAGGAAAAAAAGGTTGGC
AATATACCTCTTCTGAATACATGAAAGGAATCCAAGGGCGCGTAGATGTCAGCGTTTGGTATTAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Vegard Eldholm et al. (2009) Fratricide in Streptococcus pneumoniae: contributions and role of the cell wall hydrolases CbpD, LytA and LytC. Microbiology (Reading, England) 155(Pt 7):2223-2234. [PMID: 19389766]