Detailed information    

experimental Experimentally validated

Overview

Name   comEC/celB   Type   Machinery gene
Locus tag   SP_RS04735 Genome accession   NC_003028
Coordinates   902616..904856 (+) Length   746 a.a.
NCBI ID   WP_000942394.1    Uniprot ID   A0A166WJ67
Organism   Streptococcus pneumoniae TIGR4     
Function   ssDNA transport into the cell   
DNA binding and uptake

Function

ComEC is a transmembrane channel protein essential for the transport of extracellular DNA into the cell during natural transformation. It forms a pore in the cell membrane that allows single-stranded DNA (ssDNA) generated from internalized double-stranded DNA (dsDNA) to enter the cytoplasm. ComEC works in conjunction with ComEA to facilitate DNA uptake and processing for subsequent homologous recombination.


Genomic Context

Location: 897616..909856
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SP_RS12760 (SP_0949) - 897942..898756 (+) 815 Protein_893 IS5-like element IS1515 family transposase -
  SP_RS04705 (SP_0950) - 898939..899439 (+) 501 WP_000566988.1 GNAT family N-acetyltransferase -
  SP_RS04710 (SP_0951) - 899442..899768 (+) 327 Protein_895 TfoX/Sxy family protein -
  SP_RS12055 (SP_0952) ald 900069..901168 (-) 1100 Protein_896 alanine dehydrogenase -
  SP_RS04725 (SP_0953) - 901345..901914 (+) 570 WP_000443750.1 GNAT family N-acetyltransferase -
  SP_RS04730 (SP_0954) comEA/celA/cilE 901982..902632 (+) 651 WP_000387344.1 helix-hairpin-helix domain-containing protein Machinery gene
  SP_RS04735 (SP_0955) comEC/celB 902616..904856 (+) 2241 WP_000942394.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  SP_RS04740 (SP_0956) - 905035..905223 (+) 189 WP_001808514.1 hypothetical protein -
  SP_RS04745 (SP_0957) - 905256..905843 (+) 588 WP_000939895.1 ATP-binding cassette domain-containing protein -
  SP_RS04750 (SP_0958) - 905847..907031 (+) 1185 WP_000655960.1 ABC transporter permease -
  SP_RS04755 (SP_0959) infC 907342..907872 (+) 531 WP_000848180.1 translation initiation factor IF-3 -
  SP_RS04760 (SP_0960) rpmI 907905..908105 (+) 201 WP_001125943.1 50S ribosomal protein L35 -
  SP_RS04765 (SP_0961) rplT 908157..908516 (+) 360 WP_000124836.1 50S ribosomal protein L20 -
  SP_RS04770 (SP_0962) - 908574..908954 (+) 381 WP_000157154.1 lactoylglutathione lyase -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  htrA comEC/celB negative effect
  htrA comC/comC2 negative effect
  comC/comC2 comD/comD2 positive effect
  comD/comD2 comE positive effect
  comE comE positive effect
  comE comX/comX1 positive effect
  comE comW positive effect
  comE comA positive effect
  comE comB positive effect
  comE comM positive effect
  comE comX/comX2 positive effect
  comE comD/comD2 positive effect
  comE comC/comC2 positive effect
  stkP comE positive effect
  comX/comX1 late competence genes positive effect
  clpP comX/comX1 negative effect
  comW comX/comX1 positive effect
  clpE comX/comX1 negative effect
  comW comX/comX2 positive effect
  clpP comW negative effect
  mecA comW negative effect
  clpC comW negative effect
  comA comC/comC2 positive effect
  comB comC/comC2 positive effect
  comM cbpD negative effect
  comX/comX2 late competence genes positive effect
  clpP comX/comX2 negative effect
  clpE comX/comX2 negative effect
  ciaH comC/comC2 negative effect
  ciaR comC/comC2 negative effect
  comX/comX2 late competence genes positive effect
  comX/comX1 late competence genes positive effect
  cbpD lytA positive effect
  cbpD lytC positive effect
  ciaH htrA positive effect
  htrA comEA/celA/cilE negative effect
  ciaR htrA positive effect

Sequence

Protein


Download         Length: 746 a.a.        Molecular weight: 84444.00 Da        Isoelectric Point: 9.6202

>NTDB_id=154 SP_RS04735 WP_000942394.1 902616..904856(+) (comEC/celB) [Streptococcus pneumoniae TIGR4]
MLQWIKNFSIPLIYLSFLLLWLYYAIFSASYLALLGFVFLLVCLFIQFPWKSAGKVLIICGIFGFWFVFQNWQQSQASQN
LADSVERVRILPDTIKVNGDSLSFRGKSNGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
LKTQGIYQTLNIKTIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPDPMGNYMTGLLLGHLDTDFEEMNELYSSLGIIHL
FALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPFSLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIV
MPNFFLTAGGVLSCAYAFILTMTSKEGEGLKAVTSESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDLVFLPLLSI
LFVLSFLYPVIQLNFIFEWLEGIIRLVSQVARRPLVFGQPNAWLLILLLISLALVYDLRKNIKGLTVLSLLITGLFFLTK
YPLENEITMLDVGQGESIFLRDVTGKTILIDVGGKAESYKKIKKWQEKMTTSNAQRTLIPYLKSRGVAKIDQLILTNTDK
EHVGDLSEMTKAFHVGEILVSKDSLKQKEFVAELQATQTKVRSMIVGENLPIFGSQLEVLSPRKMGDGGHDDTLVLYGKF
LDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSSSPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGIN
SKVYRTDQQGAIRFKGLDSWKIESVR

Nucleotide


Download         Length: 2241 bp        

>NTDB_id=154 SP_RS04735 WP_000942394.1 902616..904856(+) (comEC/celB) [Streptococcus pneumoniae TIGR4]
ATGTTACAGTGGATTAAGAATTTCTCTATTCCCCTAATTTACCTGAGTTTTCTATTACTTTGGCTTTATTACGCTATTTT
CTCAGCATCTTATCTTGCTTTGTTGGGCTTTGTTTTTCTGCTAGTCTGTCTCTTTATCCAATTTCCGTGGAAATCTGCTG
GTAAAGTTCTAATAATTTGCGGAATCTTTGGATTTTGGTTTGTTTTTCAAAATTGGCAACAGAGTCAAGCGAGTCAAAAT
CTGGCGGATTCTGTTGAAAGGGTACGGATTTTGCCTGATACTATTAAGGTTAATGGTGATAGTCTATCCTTTCGTGGCAA
GTCTAACGGTCGTGCTTTCCAAGTCTATTATAAACTCCAGTCCGAGGAGGAGAAAGAAGCCTTTCAAGCTTTAACTGACC
TGCATGAGATAGGACTAGAAGGGAAGCTTTCGGAGCCAGAAGGGCAGAGAAATTTTGGTGGCTTTAATTACCAAGCCTAT
CTGAAGACTCAGGGAATTTACCAGACTCTCAATATCAAAACAATCCAGTCACTTCAAAAGATTGGCAGTTGGGATATAGG
AGAAAACTTGTCCAGTTTACGTCGAAAGGCTGTGGTTTGGATTAAGACGCACTTTCCAGACCCTATGGGCAATTACATGA
CAGGACTCTTGCTGGGACATCTGGACACCGACTTTGAGGAGATGAATGAGCTTTATTCCAGTCTAGGAATTATCCACCTC
TTTGCCCTATCTGGCATGCAGGTAGGTTTTTTCATGAATGGATTTAAGAAACTTCTCTTGCGATTGGGCTTGACCCAAGA
AAAGTTGAAATGGCTGACTTATCCCTTTTCCCTTATCTATGCGGGACTAACTGGATTTTCAGCATCGGTTATTCGCAGTC
TCTTGCAAAAGCTACTGGCTCAACATGGGGTTAAGGGCTTGGATAATTTTGCCTTGACGGTGCTTGTCCTCTTTATTGTC
ATGCCAAACTTTTTCTTGACAGCAGGAGGAGTCTTGTCCTGCGCTTATGCTTTTATCCTGACCATGACCAGCAAAGAAGG
GGAGGGGCTCAAGGCTGTTACTAGTGAAAGTCTAGTCATCTCCTTGGGCATATTGCCCATTCTATCCTTCTATTTTGCGG
AATTTCAACCTTGGTCTATCCTTTTGACCTTTGTCTTTTCCTTTCTTTTTGACTTGGTCTTCTTACCGCTCTTGTCTATC
TTATTTGTCCTTTCCTTTCTCTATCCAGTCATTCAGCTGAACTTTATCTTTGAATGGTTAGAGGGCATTATTCGCTTGGT
CTCGCAGGTGGCAAGGAGACCACTTGTCTTTGGTCAACCCAACGCATGGCTTTTAATCTTATTGTTAATTTCCTTGGCTT
TGGTCTATGATTTGAGGAAAAACATTAAAGGATTAACAGTATTGAGTTTATTGATTACAGGTCTCTTTTTCCTTACCAAG
TATCCACTGGAAAATGAAATCACCATGCTGGATGTGGGGCAAGGAGAAAGTATTTTCCTACGGGATGTAACTGGGAAAAC
CATTCTCATAGATGTAGGTGGTAAGGCAGAATCTTATAAGAAAATCAAAAAATGGCAAGAAAAGATGACGACCAGCAATG
CCCAGCGAACCTTGATTCCCTATCTCAAAAGTCGAGGAGTAGCTAAGATTGACCAGCTAATTTTGACTAACACGGACAAG
GAGCATGTTGGAGATTTGTCAGAGATGACCAAGGCTTTCCATGTAGGGGAGATTCTAGTATCAAAAGACAGTCTGAAACA
GAAGGAATTTGTGGCAGAACTACAGGCGACTCAAACAAAGGTGCGTAGTATGATAGTAGGGGAGAACTTGCCCATTTTTG
GAAGTCAGTTAGAAGTTCTATCTCCAAGGAAAATGGGAGATGGAGGACACGATGATACCCTAGTTCTGTATGGGAAATTC
TTGGATAAGCAATTTCTCTTCACGGGAAATTTGGAGGAGAAAGGAGAGAAGGACTTGCTGAAGCACTATCCAGACTTGAA
AGTAAATGTTTTGAAAGCTAGCCAACATGGCAATAAAAAATCATCAAGTCCAGCCTTTCTAGAAAAACTCAAACCAGAGC
TTACTCTTATCTCAGTTGGAAAGAGCAATCGAATGAAACTCCCCCATCAGGAAACATTGACACGACTGGAAGGTATCAAT
AGCAAAGTTTATCGAACTGACCAGCAAGGAGCTATACGTTTTAAGGGGTTGGATAGTTGGAAAATCGAAAGTGTTCGATA
G

Domains

Predicted by InterproScan.

Competence protein (Competence)

(216-458)

Domain of unknown function (DUF4131) (DUF4131)

(30-176)

Metallo-beta-lactamase superfamily (Lactamase_B)

(492-693)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A166WJ67

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEC/celB Streptococcus pneumoniae D39

97.587

100

0.976
  comEC/celB Streptococcus pneumoniae Rx1

97.587

100

0.976
  comEC/celB Streptococcus pneumoniae R6

97.587

100

0.976
  comEC/celB Streptococcus mitis SK321

92.225

100

0.922
  comEC/celB Streptococcus mitis NCTC 12261

91.812

99.866

0.917
  comEC Lactococcus lactis subsp. cremoris KW2

44.399

100

0.447

Multiple sequence alignment    



References

[1] Yanni Liu et al. (2019) HtrA-mediated selective degradation of DNA uptake apparatus accelerates termination of pneumococcal transformation. Molecular Microbiology 112(4):1308-1325. [PMID: 31396996]
[2] Scott N Peterson et al. (2004) Identification of competence pheromone responsive genes in Streptococcus pneumoniae by use of DNA microarrays. Molecular Microbiology 51(4):1051-70. [PMID: 14763980]
[3] E V Pestova et al. (1998) Isolation and characterization of three Streptococcus pneumoniae transformation-specific loci by use of a lacZ reporter insertion vector. Journal of Bacteriology 180(10):2701-10. [PMID: 9573156]