Detailed information    

experimental Experimentally validated

Overview

Name   comEC   Type   Machinery gene
Locus tag   KW2_RS08720 Genome accession   NC_022369
Coordinates   1845476..1847686 (-) Length   736 a.a.
NCBI ID   WP_021037686.1    Uniprot ID   -
Organism   Lactococcus lactis subsp. cremoris KW2     
Function   ssDNA transport into the cell   
DNA binding and uptake

Function

ComX induce the expression of late competence genes.


Genomic Context

Location: 1840476..1852686
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KW2_RS08690 (kw2_1744) atpA 1840517..1842019 (-) 1503 WP_014572214.1 F0F1 ATP synthase subunit alpha -
  KW2_RS08695 (kw2_1745) - 1842208..1842735 (-) 528 WP_014572213.1 F0F1 ATP synthase subunit delta -
  KW2_RS08700 (kw2_1746) atpF 1842737..1843243 (-) 507 WP_010906128.1 F0F1 ATP synthase subunit B -
  KW2_RS08705 (kw2_1747) atpB 1843258..1843971 (-) 714 WP_004255255.1 F0F1 ATP synthase subunit A -
  KW2_RS08710 (kw2_1748) - 1844018..1844233 (-) 216 WP_004255250.1 F0F1 ATP synthase subunit C -
  KW2_RS08715 (kw2_1749) - 1844416..1845192 (-) 777 WP_014572211.1 alpha/beta hydrolase -
  KW2_RS08720 (kw2_1750) comEC 1845476..1847686 (-) 2211 WP_021037686.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  KW2_RS08725 (kw2_1751) comEA 1847667..1848323 (-) 657 WP_021037687.1 helix-hairpin-helix domain-containing protein Machinery gene
  KW2_RS08730 (kw2_1752) - 1848381..1849742 (-) 1362 WP_021037688.1 ABC transporter permease -
  KW2_RS08735 (kw2_1753) - 1849739..1850671 (-) 933 WP_021037689.1 ABC transporter ATP-binding protein -
  KW2_RS08740 (kw2_1754) - 1850777..1851175 (-) 399 WP_021037690.1 hypothetical protein -
  KW2_RS08745 (kw2_1755) - 1851293..1851853 (-) 561 WP_021037691.1 GNAT family N-acetyltransferase -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  comX comEC positive effect
  comX comGG positive effect
  comX comEA positive effect
  comX ssbB positive effect
  comX comGD positive effect
  comX dprA positive effect
  comX comFA positive effect
  comX coiA positive effect
  comX comGB positive effect
  comX recA positive effect
  comX comFC positive effect
  comX comGE positive effect
  comX comGF positive effect
  comX comGA positive effect
  comX comGC positive effect
  mecA comX negative effect
  clpC comX negative effect
  clpP comX negative effect

Sequence

Protein


Download         Length: 736 a.a.        Molecular weight: 84581.59 Da        Isoelectric Point: 9.8042

>NTDB_id=596 KW2_RS08720 WP_021037686.1 1845476..1847686(-) (comEC) [Lactococcus lactis subsp. cremoris KW2]
MRFLLIKFPLIFLTYLVVLLYFCIFTYNLPLLILFICSILLALFRKYYLMIPILIVFAVFFLLINSLSIRDNENQPKTLS
KIELISDTIQVNGDLLSFQGRMNGQNYQVYDTLKSLKEKKFYQNLSQNCQLSFTGNLQIPETQRNFNGFDDQKYLASQNI
YRQITIQQITNVEIKKNFDLHTLRRKAIVWTQNHFPKPMSDYMTGLLFGFLSKDFNQMSDIYSSLGIIHLFALSGMQVNF
FIDWFRKILLRMGLTREIVNLVQIPFSIFYAFMTGLSVSVLRALFQKNIRLTSLDNIGVTTLVLMLISPKFLLTTGGQLT
LFYAFVISMLNHPFSNLNGLKKVLTESAVLSLCVLPLLIFDFHIFQPLSILLTIIFGFLFDFMLLPLLLCCFLISFLGFS
LNLNFIFQILEWLVHKVDLPLHYPFVLGKPSSLELIILFVLIGLFVDSFSHKKRRFFLVGFIFLFFFITKNPMRASITAV
DIGQGDSIFLQDKFNKENILIDTGGRLALPQKNWQKAQTQSNADKTLIPYLESMGVAHIDQLILTHTDADHVGDFLSLAD
KIKIREIWVSPGELTNSSFVEKLKKAKIPIHVSKVGDKIPIFDSFLQVLSNGYTKKGDNNDSIVTYGNFYHTKFLFTGDL
EQEGEKKLLQNYPKLQVDVLKVGHHGSKTSSSPEFIKEIHPKLALISVGKANRYGHPNQETLETLAKYQVKTLRTDQKGA
IKLTEVNQQWQIKTVR

Nucleotide


Download         Length: 2211 bp        

>NTDB_id=596 KW2_RS08720 WP_021037686.1 1845476..1847686(-) (comEC) [Lactococcus lactis subsp. cremoris KW2]
ATGAGATTTCTATTGATTAAATTTCCCCTGATTTTTCTAACTTATCTTGTTGTGCTCCTTTATTTTTGTATCTTTACTTA
CAATTTACCCTTGCTTATTTTATTTATTTGCTCAATTTTACTAGCTTTATTCAGAAAATATTATCTAATGATTCCGATAT
TAATTGTTTTTGCTGTATTTTTTCTGTTAATCAATAGTCTTAGTATTAGGGATAATGAAAATCAACCAAAAACTCTCTCA
AAAATTGAATTAATTTCAGATACAATTCAAGTCAATGGTGATTTATTGAGTTTTCAGGGGAGAATGAACGGACAAAACTA
TCAAGTCTATGACACATTGAAAAGTCTAAAAGAGAAAAAATTTTATCAGAACTTAAGTCAAAATTGTCAGTTAAGTTTCA
CAGGAAACCTTCAAATACCAGAAACTCAGAGAAATTTTAATGGTTTTGATGACCAAAAATATTTAGCTAGTCAAAATATC
TATCGACAGATTACCATCCAACAAATTACAAATGTGGAAATAAAAAAGAATTTTGACCTTCATACTTTAAGGCGAAAGGC
AATTGTTTGGACACAAAATCATTTTCCAAAACCGATGAGTGACTATATGACAGGCTTACTTTTTGGCTTTTTAAGCAAGG
ATTTTAATCAGATGAGCGATATTTACAGCTCTTTAGGAATTATTCATCTTTTTGCTCTATCAGGAATGCAAGTAAATTTT
TTTATTGATTGGTTTCGTAAAATACTTCTTCGCATGGGACTGACGAGAGAAATAGTGAATCTTGTTCAAATTCCATTTTC
TATTTTTTATGCTTTTATGACGGGATTATCAGTTTCAGTTTTAAGAGCCCTTTTTCAAAAAAATATTCGTCTGACTTCTT
TGGATAATATCGGAGTGACGACATTAGTTTTGATGCTCATAAGTCCAAAATTTTTACTGACGACTGGCGGTCAGCTTACC
TTGTTTTATGCTTTTGTCATTTCGATGCTTAACCATCCTTTTTCTAATTTAAATGGATTGAAAAAAGTGCTGACAGAATC
AGCAGTCCTTTCACTTTGTGTTCTTCCGTTACTCATCTTTGATTTCCATATTTTTCAGCCTCTTTCAATATTATTAACAA
TTATATTTGGTTTTCTTTTTGATTTTATGCTGCTCCCGCTGCTTTTATGCTGTTTTCTTATATCATTTTTGGGATTTTCG
CTCAATTTAAATTTTATTTTTCAAATTCTAGAATGGCTAGTTCATAAAGTTGATTTACCCTTACATTACCCCTTTGTTTT
GGGGAAACCTAGTTCACTCGAATTAATTATTCTATTTGTGTTAATTGGTTTATTCGTAGATTCATTTTCTCATAAAAAAA
GGAGATTTTTTTTAGTTGGATTTATATTTTTATTTTTTTTCATCACTAAAAATCCAATGAGAGCAAGCATTACAGCGGTT
GATATCGGACAAGGCGATAGTATTTTTCTACAAGATAAGTTTAATAAAGAAAATATTCTGATTGATACAGGGGGGCGACT
TGCTCTTCCTCAAAAAAATTGGCAAAAAGCGCAGACGCAATCAAATGCTGACAAAACGCTTATTCCTTATTTAGAATCCA
TGGGGGTGGCTCATATTGACCAACTTATTCTGACTCATACAGATGCTGACCATGTTGGCGATTTCTTGTCCTTAGCTGAT
AAAATAAAAATAAGAGAAATCTGGGTCAGTCCGGGAGAATTAACCAACAGTAGTTTTGTGGAAAAATTAAAAAAAGCAAA
AATTCCGATTCATGTTAGTAAAGTTGGGGATAAAATTCCGATTTTTGATAGTTTTCTTCAAGTTTTATCAAATGGTTACA
CTAAAAAAGGGGATAATAATGATTCCATAGTGACTTATGGAAACTTTTACCATACAAAATTTCTTTTTACAGGAGATTTG
GAACAAGAAGGAGAAAAAAAGCTTCTACAAAATTATCCCAAATTACAGGTAGATGTGTTAAAAGTGGGGCATCATGGCAG
TAAAACAAGTTCAAGTCCGGAATTCATCAAAGAGATACATCCTAAACTTGCACTAATCTCGGTTGGAAAAGCTAATCGCT
ATGGTCATCCCAATCAAGAAACTTTAGAAACCTTAGCCAAATATCAGGTAAAGACACTCAGAACTGACCAAAAAGGGGCA
ATAAAATTAACAGAAGTAAATCAGCAATGGCAAATCAAAACAGTTAGATAA

Domains

Predicted by InterproScan.

Metallo-beta-lactamase superfamily (Lactamase_B)

(483-689)

Domain of unknown function (DUF4131) (DUF4131)

(27-171)

Competence protein (Competence)

(206-446)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEC/celB Streptococcus mitis SK321

45.479

100

0.458
  comEC/celB Streptococcus mitis NCTC 12261

44.459

100

0.447
  comEC/celB Streptococcus pneumoniae TIGR4

44.399

100

0.447
  comEC/celB Streptococcus pneumoniae D39

43.725

100

0.44
  comEC/celB Streptococcus pneumoniae R6

43.725

100

0.44
  comEC/celB Streptococcus pneumoniae Rx1

43.725

100

0.44

Multiple sequence alignment    



References

[1] Blandine David et al. (2017) Natural DNA Transformation Is Functional in Lactococcus lactis subsp. cremoris KW2. Applied And Environmental Microbiology 83(16):e01074-17. [PMID: 28625996]