Detailed information    

experimental Experimentally validated

Overview

Name   comEC/celB   Type   Machinery gene
Locus tag   SMSK321_RS04965 Genome accession   NZ_AEDT01000012
Coordinates   27303..29543 (+) Length   746 a.a.
NCBI ID   WP_000942453.1    Uniprot ID   -
Organism   Streptococcus mitis SK321     
Function   ssDNA transport into the cell   
DNA binding and uptake

Function

Upregulated in response to CSP as measured by RNA-seq.


Genomic Context

Location: 22303..34543
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SMSK321_RS04935 (SMSK321_0416) - 22799..23356 (+) 558 WP_004234343.1 GrpB family protein -
  SMSK321_RS04940 (SMSK321_0417) - 23402..23617 (+) 216 WP_001232084.1 YozE family protein -
  SMSK321_RS04945 (SMSK321_0418) - 23698..24693 (+) 996 WP_000658203.1 PhoH family protein -
  SMSK321_RS04950 (SMSK321_0419) ald 24749..25861 (-) 1113 WP_000904691.1 alanine dehydrogenase -
  SMSK321_RS04955 (SMSK321_0420) - 26033..26602 (+) 570 WP_000443733.1 GNAT family N-acetyltransferase -
  SMSK321_RS04960 (SMSK321_0421) comEA/celA/cilE 26669..27319 (+) 651 WP_000443804.1 helix-hairpin-helix domain-containing protein Machinery gene
  SMSK321_RS04965 (SMSK321_0422) comEC/celB 27303..29543 (+) 2241 WP_000942453.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  SMSK321_RS04975 (SMSK321_0423) - 29943..30530 (+) 588 WP_000939891.1 ABC transporter ATP-binding protein -
  SMSK321_RS04980 (SMSK321_0424) - 30534..31718 (+) 1185 WP_000655927.1 hypothetical protein -
  SMSK321_RS04985 (SMSK321_0425) infC 32027..32557 (+) 531 WP_000848180.1 translation initiation factor IF-3 -
  SMSK321_RS04990 (SMSK321_0426) rpmI 32590..32790 (+) 201 WP_001125943.1 50S ribosomal protein L35 -
  SMSK321_RS04995 (SMSK321_0427) rplT 32842..33201 (+) 360 WP_000124832.1 50S ribosomal protein L20 -
  SMSK321_RS05000 (SMSK321_0428) - 33260..33640 (+) 381 WP_000157156.1 lactoylglutathione lyase -

Sequence

Protein


Download         Length: 746 a.a.        Molecular weight: 84423.84 Da        Isoelectric Point: 9.0434

>NTDB_id=522 SMSK321_RS04965 WP_000942453.1 27303..29543(+) (comEC/celB) [Streptococcus mitis SK321]
MLQWTKNFPIPLIYLSFLLLWLYYAIFSVSYLALLGFVFLLVCLFIQFLWKSAGKVLVICGIFGFWFVFQNWQQSQASQN
LSDSVEIVRILPDTIKVNGDSLSFRGKADGRIFQVYYKLQSEEEKETFQILTALHDLELEGKLSEPEGKRNFGGFDYQAY
LKTQGIYQTLNIKKIQSLQKVGSWDLGENLSSLRRKAVVWIKTHFPDPMRNYMTGLLLGHLDTDFEEMNELYSSLGIIHL
FALSGMQVGFFMDGFKKLLLRLGLTQEKLKWLTYPFSLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFII
MPNFFLTAGGVLSCAYAFILTMTSKEGEGLKAVARESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDLVFLPLLSV
LFALSFLYPVIQLNFIFEWLEGMIRLVSQVASRPLVFGQPNAWLLILLLISLALVYDLRKNIKRLAVLSLLITGLFFLTK
YPLENEITMLDVGQGESIFLRDVAGKTILIDVGGKAESDKKIEKWQEKATTSNAQRTLIPYLKSRGVAKIDQLILTNTDK
EHVGDLLEVTKAFHVGEILVSKGSLKQKEFVAELQATQIKVHSVAEGENVPIFGSQLEVLSPRKIGDGGHDDSLVLYGKL
LDKHFLFTGNLEEKGEKDLLKQYPDLEVDVLKASQHGSKKSSSSAFLEQLKPEMTLISVGKSNRMKLPHQETLTRLEGIN
SKVYRTDQQGAIRFKGWNSWKIESVR

Nucleotide


Download         Length: 2241 bp        

>NTDB_id=522 SMSK321_RS04965 WP_000942453.1 27303..29543(+) (comEC/celB) [Streptococcus mitis SK321]
ATGTTACAGTGGACTAAGAATTTCCCCATTCCCCTAATTTACCTGAGTTTTCTATTACTTTGGCTTTACTACGCTATTTT
CTCAGTATCCTATCTTGCTTTGTTGGGCTTTGTTTTTCTGCTAGTCTGTCTCTTTATCCAATTTCTTTGGAAATCTGCTG
GAAAAGTTCTAGTAATTTGTGGAATCTTTGGATTTTGGTTTGTTTTTCAAAATTGGCAACAGAGTCAAGCGAGTCAAAAT
CTGTCAGATTCTGTTGAAATTGTACGGATTTTACCTGACACTATTAAGGTTAATGGTGATAGTTTGTCCTTTCGTGGCAA
GGCTGATGGACGCATTTTTCAAGTCTATTATAAACTCCAGTCCGAGGAGGAGAAAGAAACCTTTCAAATTTTAACAGCCC
TTCATGATTTGGAACTAGAAGGGAAACTTTCGGAGCCAGAAGGGAAGAGAAATTTTGGTGGTTTTGACTATCAAGCCTAT
CTGAAAACTCAGGGAATTTACCAGACTCTAAATATCAAAAAAATCCAGTCACTTCAAAAGGTTGGCAGTTGGGATCTAGG
TGAAAACCTGTCCAGTTTACGCCGTAAGGCTGTAGTTTGGATTAAGACGCATTTTCCAGATCCTATGCGCAATTACATGA
CGGGGCTTCTATTAGGACATCTGGATACAGACTTTGAGGAGATGAATGAGCTTTATTCTAGTCTAGGAATTATCCACCTA
TTTGCCTTGTCAGGCATGCAGGTAGGATTTTTCATGGATGGATTTAAGAAACTACTCTTGCGATTAGGCTTGACCCAAGA
AAAGTTGAAATGGCTGACTTATCCCTTTTCCCTTATCTATGCAGGGCTGACAGGATTTTCAGCATCGGTCATTCGCAGTC
TCTTGCAAAAGTTACTGGCTCAACATGGTGTTAAGGGTTTGGATAATTTTGCCTTGACGGTTCTTGTCCTCTTTATCATC
ATGCCAAACTTTTTCTTGACAGCAGGAGGAGTCTTGTCCTGTGCTTATGCTTTTATCTTGACCATGACCAGCAAAGAAGG
AGAGGGCCTCAAGGCTGTGGCTAGAGAAAGTCTAGTCATTTCCTTGGGAATATTGCCCATTCTGTCCTTCTATTTTGCGG
AATTTCAACCTTGGTCCATCCTCTTGACCTTTGTCTTTTCCTTTCTTTTTGACTTGGTCTTCTTACCGCTTTTGTCTGTC
TTATTTGCCCTTTCCTTTCTCTACCCAGTCATTCAGCTTAATTTTATTTTTGAATGGTTGGAGGGGATGATTCGCTTGGT
ATCGCAGGTGGCAAGTAGACCGCTTGTCTTTGGACAACCTAATGCGTGGCTTTTAATCTTATTGTTAATTTCCTTGGCTT
TGGTCTATGATTTGAGGAAAAACATTAAAAGGCTAGCAGTATTGAGCTTATTGATTACAGGGCTCTTTTTCCTTACCAAG
TATCCACTGGAAAATGAAATTACCATGCTGGATGTGGGGCAAGGGGAAAGTATTTTCCTACGGGATGTAGCTGGTAAAAC
CATTCTCATAGATGTCGGTGGCAAGGCAGAATCTGATAAGAAAATTGAAAAATGGCAAGAAAAGGCAACGACCAGCAATG
CCCAGCGAACCTTGATTCCTTACCTCAAAAGTCGAGGAGTAGCCAAGATTGACCAGCTGATTTTGACAAATACGGACAAG
GAACATGTTGGAGATTTGTTGGAGGTGACCAAGGCTTTCCATGTAGGCGAAATTTTAGTGTCCAAAGGCAGTTTGAAACA
GAAGGAATTTGTGGCAGAACTACAGGCGACTCAAATTAAGGTGCATAGTGTGGCAGAAGGGGAAAATGTGCCAATTTTTG
GAAGTCAGTTAGAAGTCCTATCTCCGAGAAAAATTGGAGATGGAGGTCATGATGATTCTCTGGTTCTGTATGGAAAACTC
TTGGATAAGCACTTTCTCTTCACGGGAAATTTGGAGGAGAAAGGAGAGAAGGATTTGCTGAAGCAATATCCTGACCTGGA
GGTGGATGTTTTGAAAGCTAGCCAACATGGCTCTAAAAAATCATCAAGTTCAGCCTTTCTAGAACAGTTAAAACCAGAGA
TGACTCTCATCTCAGTTGGAAAGAGCAATAGAATGAAACTCCCCCATCAGGAAACCTTGACACGACTGGAAGGTATCAAT
AGCAAAGTTTACCGAACTGACCAGCAAGGAGCTATACGCTTTAAAGGTTGGAATAGTTGGAAGATCGAAAGTGTTCGATA
G

Domains

Predicted by InterproScan.

Competence protein (Competence)

(216-458)

Domain of unknown function (DUF4131) (DUF4131)

(29-177)

Metallo-beta-lactamase superfamily (Lactamase_B)

(492-693)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEC/celB Streptococcus pneumoniae TIGR4

92.225

100

0.922
  comEC/celB Streptococcus pneumoniae D39

91.823

100

0.918
  comEC/celB Streptococcus pneumoniae R6

91.823

100

0.918
  comEC/celB Streptococcus pneumoniae Rx1

91.823

100

0.918
  comEC/celB Streptococcus mitis NCTC 12261

91.946

99.866

0.918
  comEC Lactococcus lactis subsp. cremoris KW2

45.479

100

0.458

Multiple sequence alignment    



References

[1] G Salvadori et al. (2018) High-resolution profiles of the Streptococcus mitis CSP signaling pathway reveal core and strain-specific regulated genes. BMC Genomics 19(1):453. [PMID: 29898666]