Detailed information NTG0000521

Experimentally validated

Overview

• Name: comEA/celA/cilE
• Type: Machinery gene
• Locus tag: SMSK321_RS04960
• Genome accession: NZ_AEDT01000012
• Coordinates: 26669..27319 (+)
• Length: 216 a.a.
• NCBI ID: WP_000443804.1
• Uniprot ID: -
• Organism: Streptococcus mitis SK321
• Function: dsDNA binding to the cell surface DNA binding and uptake

Function

Upregulated in response to CSP as measured by RNA-seq.

Genomic Context

Location: 21669..32319

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SMSK321_RS04930 (SMSK321_0415)	cvfB	21933..22787 (+)	855	WP_001095459.1	RNA-binding virulence regulatory protein CvfB	-
SMSK321_RS04935 (SMSK321_0416)	-	22799..23356 (+)	558	WP_004234343.1	GrpB family protein	-
SMSK321_RS04940 (SMSK321_0417)	-	23402..23617 (+)	216	WP_001232084.1	YozE family protein	-
SMSK321_RS04945 (SMSK321_0418)	-	23698..24693 (+)	996	WP_000658203.1	PhoH family protein	-
SMSK321_RS04950 (SMSK321_0419)	ald	24749..25861 (-)	1113	WP_000904691.1	alanine dehydrogenase	-
SMSK321_RS04955 (SMSK321_0420)	-	26033..26602 (+)	570	WP_000443733.1	GNAT family N-acetyltransferase	-
SMSK321_RS04960 (SMSK321_0421)	comEA/celA/cilE	26669..27319 (+)	651	WP_000443804.1	helix-hairpin-helix domain-containing protein	Machinery gene
SMSK321_RS04965 (SMSK321_0422)	comEC/celB	27303..29543 (+)	2241	WP_000942453.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
SMSK321_RS04975 (SMSK321_0423)	-	29943..30530 (+)	588	WP_000939891.1	ABC transporter ATP-binding protein	-
SMSK321_RS04980 (SMSK321_0424)	-	30534..31718 (+)	1185	WP_000655927.1	hypothetical protein	-

Sequence

Protein

• Length: 216 a.a.
• Molecular weight: 23217.47 Da
• Isoelectric Point: 6.7366

>NTDB_id=521 SMSK321_RS04960 WP_000443804.1 26669..27319(+) (comEA/celA/cilE) [Streptococcus mitis SK321]
MESIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPHTPVKETNLQAEVVAISKDSSTEKDVKKEEKEEPVEQDLITVDVKG
AVKSPGIYDLSVGSRVNDAVQKAGGLTEQADSKSLNLAQKVSDEALVYVPTKGEESASQHTGSGAPSSTSKDKKINLNKA
SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGAKTIEKLKDYVTVD

Nucleotide

• Length: 651 bp

>NTDB_id=521 SMSK321_RS04960 WP_000443804.1 26669..27319(+) (comEA/celA/cilE) [Streptococcus mitis SK321]
ATGGAATCAATTATCGAGAAAATCAAAGAGTATAAAATCATTGTCATCTGTACTGGGCTGGGCTTGCTTGTAGGCGGATT
TTTCCTGCTAAAGCCAGCTCCACACACACCTGTCAAAGAAACGAATTTGCAGGCAGAAGTTGTAGCAATTTCTAAGGATT
CATCGACCGAAAAGGATGTGAAGAAGGAAGAAAAGGAAGAACCTGTTGAACAAGATCTAATCACAGTAGATGTCAAAGGT
GCTGTAAAATCACCAGGGATTTATGATTTGTCTGTAGGTAGTCGGGTCAATGACGCTGTTCAAAAGGCGGGTGGCTTGAC
AGAGCAAGCAGACAGCAAGTCGCTCAATCTAGCTCAGAAAGTTAGTGATGAAGCTCTGGTTTACGTTCCCACTAAGGGAG
AAGAATCAGCTAGTCAACATACTGGTTCTGGAGCGCCTTCTTCAACAAGCAAGGACAAGAAGATCAATCTCAACAAAGCC
AGTCTGGAAGAACTCAAGCAGGTCAAAGGACTGGGTGGCAAACGAGCTCAGGACATTATCGACCATCGTGAGGCGAATGG
GAAGTTCAAGTCGGTCGATGAACTCAAGAAGGTCTCTGGCATTGGCGCCAAAACGATAGAAAAGTTAAAAGACTATGTTA
CAGTGGACTAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (76-126)

• Helix-hairpin-helix motif (HHH_3) (152-214)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	93.056	100	0.931
comEA/celA/cilE	Streptococcus mitis NCTC 12261	92.593	100	0.926
comEA/celA/cilE	Streptococcus pneumoniae R6	90.278	100	0.903
comEA/celA/cilE	Streptococcus pneumoniae Rx1	90.278	100	0.903
comEA/celA/cilE	Streptococcus pneumoniae D39	90.278	100	0.903
comEA	Streptococcus thermophilus LMD-9	41.739	100	0.444
comEA	Lactococcus lactis subsp. cremoris KW2	41.778	100	0.435
Cj0011c	Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819	47.619	79.747	0.38

References

• [1]: G Salvadori et al. (2018) High-resolution profiles of the Streptococcus mitis CSP signaling pathway reveal core and strain-specific regulated genes. BMC Genomics 19(1):453. [PMID: 29898666]