Detailed information    

experimental Experimentally validated

Overview

Name   comEA/celA/cilE   Type   Machinery gene
Locus tag   SM12261_RS04300 Genome accession   NZ_CP028414
Coordinates   794902..795552 (+) Length   216 a.a.
NCBI ID   WP_000387351.1    Uniprot ID   A0AAX2L5S7
Organism   Streptococcus mitis NCTC 12261     
Function   dsDNA binding to the cell surface   
DNA binding and uptake

Function

Upregulated in response to CSP as measured by RNA-seq.


Genomic Context

Location: 789902..800552
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SM12261_RS04270 (SM12261_0829) frr 790150..790707 (+) 558 WP_001262225.1 ribosome recycling factor -
  SM12261_RS04275 (SM12261_0830) cvfB 790767..791621 (+) 855 WP_001095458.1 RNA-binding virulence regulatory protein CvfB -
  SM12261_RS04280 (SM12261_0831) - 791630..791845 (+) 216 WP_001232083.1 YozE family protein -
  SM12261_RS04285 (SM12261_0832) - 791930..792925 (+) 996 WP_000658173.1 PhoH family protein -
  SM12261_RS04290 (SM12261_0833) ald 792982..794094 (-) 1113 WP_000904719.1 alanine dehydrogenase -
  SM12261_RS04295 (SM12261_0834) - 794266..794835 (+) 570 WP_000443732.1 GNAT family N-acetyltransferase -
  SM12261_RS04300 (SM12261_0835) comEA/celA/cilE 794902..795552 (+) 651 WP_000387351.1 helix-hairpin-helix domain-containing protein Machinery gene
  SM12261_RS04305 (SM12261_0836) comEC/celB 795536..797776 (+) 2241 WP_000942409.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  SM12261_RS04310 (SM12261_0837) infC 797996..798526 (+) 531 WP_000848180.1 translation initiation factor IF-3 -
  SM12261_RS04315 (SM12261_0838) rpmI 798559..798759 (+) 201 WP_001125943.1 50S ribosomal protein L35 -
  SM12261_RS04320 (SM12261_0839) rplT 798811..799170 (+) 360 WP_000124834.1 50S ribosomal protein L20 -

Sequence

Protein


Download         Length: 216 a.a.        Molecular weight: 23173.54 Da        Isoelectric Point: 6.0746

>NTDB_id=493 SM12261_RS04300 WP_000387351.1 794902..795552(+) (comEA/celA/cilE) [Streptococcus mitis NCTC 12261]
MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQAEVVAVSKDSVSEKEVKKEEKEEPLEQDLITVDVKG
AVKSPGIYDLPVGSRVNDAVQKAGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAASQQAGPGTASSTSKEKKVNLNKA
SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGAKTIEKLKDYVTVD

Nucleotide


Download         Length: 651 bp        

>NTDB_id=493 SM12261_RS04300 WP_000387351.1 794902..795552(+) (comEA/celA/cilE) [Streptococcus mitis NCTC 12261]
ATGGAAGCAATTATCGAGAAAATCAAAGAGTATAAAATCATTGTTATCTGTACTGGTCTGGGATTACTTGTCGGAGGATT
TTTCCTGCTAAAGCCAGCTCCACAAACACCTGTCAAGGAAACGAATTTGCAGGCAGAAGTTGTAGCAGTTTCCAAGGATT
CGGTATCCGAAAAGGAAGTAAAGAAGGAAGAAAAGGAAGAACCCCTTGAACAAGATCTAATCACAGTAGATGTCAAAGGT
GCTGTTAAATCGCCAGGGATTTATGATTTGCCTGTAGGTAGTCGAGTCAATGATGCTGTTCAGAAGGCTGGTGGCTTGAC
AGAACAAGCAGATAGCAAGTCGCTCAATCTAGCTCAGAAAGTTAGTGACGAGGCTCTGGTTTACGTCCCAACTAAGGGAG
AAGAAGCAGCTAGTCAGCAGGCAGGTCCTGGGACGGCTTCCTCAACGAGCAAGGAAAAGAAGGTCAATCTCAATAAGGCC
AGTCTGGAAGAACTCAAGCAGGTCAAAGGACTGGGTGGCAAACGAGCTCAGGATATTATCGACCATCGTGAGGCAAATGG
GAAATTCAAGTCGGTTGATGAACTCAAGAAAGTCTCTGGCATTGGTGCCAAGACCATAGAAAAGTTAAAAGATTATGTTA
CAGTGGATTAA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(76-126)

Helix-hairpin-helix motif (HHH_3)

(151-214)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

96.759

100

0.968
  comEA/celA/cilE Streptococcus pneumoniae Rx1

96.296

100

0.963
  comEA/celA/cilE Streptococcus pneumoniae D39

96.296

100

0.963
  comEA/celA/cilE Streptococcus pneumoniae R6

96.296

100

0.963
  comEA/celA/cilE Streptococcus mitis SK321

92.593

100

0.926
  comEA Lactococcus lactis subsp. cremoris KW2

40.889

100

0.426
  Cj0011c Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

49.206

79.747

0.392
  comEA Streptococcus thermophilus LMD-9

37.719

98.701

0.372
  comEA Latilactobacillus sakei subsp. sakei 23K

34.335

100

0.37

Multiple sequence alignment    



References

[1] G Salvadori et al. (2018) High-resolution profiles of the Streptococcus mitis CSP signaling pathway reveal core and strain-specific regulated genes. BMC Genomics 19(1):453. [PMID: 29898666]