Detailed information NTG0000300

Experimentally validated

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: STER_RS07485
• Genome accession: NC_008532
• Coordinates: 1426025..1426720 (-)
• Length: 231 a.a.
• NCBI ID: WP_011681496.1
• Uniprot ID: -
• Organism: Streptococcus thermophilus LMD-9
• Function: dsDNA binding to the cell surface DNA binding and uptake

Function

The maximum transformation efficiency was obtained by overexpression of comEA, which was 14-fold higher than that of control.

Genomic Context

Location: 1421025..1431720

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
STER_RS07470 (STER_1518)	-	1421836..1422381 (-)	546	WP_011681493.1	GNAT family N-acetyltransferase	-
STER_RS07475 (STER_1519)	-	1422447..1423709 (-)	1263	WP_011681494.1	UDP-N-acetylglucosamine 1-carboxyvinyltransferase	-
STER_RS07480 (STER_1520)	comEC	1423795..1426035 (-)	2241	WP_011681495.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
STER_RS07485 (STER_1521)	comEA	1426025..1426720 (-)	696	WP_011681496.1	helix-hairpin-helix domain-containing protein	Machinery gene
STER_RS07490 (STER_1522)	-	1426828..1427583 (-)	756	WP_011681497.1	lysophospholipid acyltransferase family protein	-
STER_RS07495 (STER_1523)	-	1427774..1428649 (+)	876	WP_370869446.1	polysaccharide deacetylase family protein	-
STER_RS07500 (STER_1524)	-	1428699..1429463 (+)	765	WP_011681498.1	tRNA1(Val) (adenine(37)-N6)-methyltransferase	-
STER_RS07505 (STER_1525)	-	1429467..1429736 (+)	270	WP_011226404.1	GIY-YIG nuclease family protein	-

Sequence

Protein

• Length: 231 a.a.
• Molecular weight: 24470.41 Da
• Isoelectric Point: 4.6300

>NTDB_id=300 STER_RS07485 WP_011681496.1 1426025..1426720(-) (comEA) [Streptococcus thermophilus LMD-9]
MKEKILAYVKDNRLFVSVIAVLMVIFCFFLWMTCGAGNSMEAETSYTDVTALSTSSSKQSSQSLSEASSQSKTEGSEKVK
SKVTVDVKGAVVNPGVYTLKAGARVTDVIQEAGGMTEDADAKSVNLAASLSDEEVIYVANKDENVSVLDQTGTGQVSDKG
GQAVSKDGKINLNTATSEQLQTISGIGAKRAEDVIAYRESHGGFQSVDDLKNVSGIGDKTLDKIRESLYVA

Nucleotide

• Length: 696 bp

>NTDB_id=300 STER_RS07485 WP_011681496.1 1426025..1426720(-) (comEA) [Streptococcus thermophilus LMD-9]
GTGAAGGAAAAGATTCTAGCCTATGTCAAAGATAATCGTCTGTTTGTGAGTGTCATCGCTGTACTGATGGTGATTTTTTG
CTTCTTTTTATGGATGACTTGTGGTGCCGGCAACAGCATGGAGGCGGAGACGTCTTATACAGATGTGACAGCTTTGTCAA
CGTCTTCATCCAAACAAAGTTCACAGTCTCTTTCTGAGGCGTCTTCCCAGTCAAAGACCGAAGGAAGTGAAAAAGTTAAG
TCAAAAGTAACGGTAGATGTTAAGGGGGCTGTGGTCAATCCAGGTGTCTATACGCTAAAGGCAGGCGCTAGGGTGACAGA
TGTCATTCAGGAAGCTGGAGGAATGACAGAAGATGCAGACGCTAAGAGTGTTAACTTAGCCGCAAGCTTGTCAGATGAAG
AGGTTATTTATGTAGCCAATAAAGATGAAAATGTTTCTGTCCTTGATCAAACAGGTACTGGTCAGGTCTCTGACAAAGGA
GGGCAGGCTGTATCTAAGGATGGCAAAATTAACTTAAATACAGCGACCTCAGAGCAGTTGCAAACCATTTCTGGAATTGG
AGCTAAGCGGGCAGAGGATGTCATTGCCTATCGTGAAAGTCATGGCGGCTTTCAGTCCGTAGATGACTTGAAAAATGTCT
CAGGAATTGGTGATAAAACATTAGATAAAATCAGAGAGTCCCTCTATGTGGCTTAA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (167-228)

• SLBB domain (SLBB) (85-138)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA/celA/cilE	Streptococcus mitis SK321	41.739	100	0.444
comEA/celA/cilE	Streptococcus pneumoniae Rx1	39.035	98.701	0.385
comEA/celA/cilE	Streptococcus pneumoniae R6	39.035	98.701	0.385
comEA/celA/cilE	Streptococcus pneumoniae D39	39.035	98.701	0.385
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	39.035	98.701	0.385
comEA/celA/cilE	Streptococcus mitis NCTC 12261	37.719	98.701	0.372

References

• [1]: Ling-Hui Kong et al. (2021) High-efficiency transformation of Streptococcus thermophilus using electroporation. Journal of The Science of Food And Agriculture 101(15):6578-6585. [PMID: 33937994]