Detailed information    

experimental Experimentally validated

Overview

Name   comEA/celA/cilE   Type   Machinery gene
Locus tag   SPR_RS04295 Genome accession   NC_003098
Coordinates   849966..850616 (+) Length   216 a.a.
NCBI ID   WP_000387330.1    Uniprot ID   -
Organism   Streptococcus pneumoniae R6     
Function   dsDNA binding to the cell surface   
DNA binding and uptake

Function

ComEA is a DNA-binding protein critical for the uptake of extracellular DNA during natural transformation. It binds to double-stranded DNA (dsDNA) at the cell surface, facilitating its transport into the cell through the competence-specific DNA uptake machinery. ComEA works in concert with other proteins such as ComEC, which is involved in the transport of single-stranded DNA into the cell.


Genomic Context

Location: 844966..855616
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPR_RS04250 (spr0848) - 845449..845664 (+) 216 WP_001232082.1 YozE family protein -
  SPR_RS04255 (spr0849) - 845750..846718 (+) 969 WP_000658183.1 PhoH family protein -
  SPR_RS04265 (spr0850) - 846911..847411 (+) 501 WP_000566988.1 GNAT family N-acetyltransferase -
  SPR_RS04270 (spr0851) - 847414..847740 (+) 327 Protein_845 TfoX/Sxy family protein -
  SPR_RS10730 ald 848041..849152 (-) 1112 Protein_846 alanine dehydrogenase -
  SPR_RS04290 (spr0855) - 849329..849898 (+) 570 WP_000443899.1 GNAT family N-acetyltransferase -
  SPR_RS04295 (spr0856) comEA/celA/cilE 849966..850616 (+) 651 WP_000387330.1 ComEA family DNA-binding protein Machinery gene
  SPR_RS04300 (spr0857) comEC/celB 850600..852840 (+) 2241 WP_000942407.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  SPR_RS04305 (spr0858) - 853019..853207 (+) 189 WP_010976492.1 hypothetical protein -
  SPR_RS04310 (spr0859) - 853239..853826 (+) 588 Protein_851 ATP-binding cassette domain-containing protein -
  SPR_RS04315 (spr0860) - 853830..855011 (+) 1182 WP_000655958.1 ABC transporter permease -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  htrA comEA/celA/cilE negative effect
  htrA comC/comC1 negative effect
  comC/comC1 comD/comD1 positive effect
  comD/comD1 comE positive effect
  comE comB positive effect
  comB comC/comC1 positive effect
  comA comC/comC1 positive effect
  ciaH comC/comC1 negative effect
  ciaR comC/comC1 negative effect
  comE comC/comC1 positive effect
  comE comW positive effect
  comE comX/comX1 positive effect
  comE comA positive effect
  comE comD/comD1 positive effect
  comE comX/comX2 positive effect
  comE comM positive effect
  comE comE positive effect
  stkP comE positive effect
  comW comX/comX1 positive effect
  comX/comX1 late competence genes positive effect
  clpE comX/comX1 negative effect
  clpP comX/comX1 negative effect
  comW comX/comX2 positive effect
  comX/comX2 late competence genes positive effect
  clpE comX/comX2 negative effect
  clpP comX/comX2 negative effect
  clpP comW negative effect
  mecA comW negative effect
  clpC comW negative effect
  ciaH htrA positive effect
  ciaR htrA positive effect
  htrA comEC/celB negative effect
  comX/comX2 late competence genes positive effect
  comX/comX1 late competence genes positive effect
  comM cbpD negative effect
  cbpD lytC positive effect
  cbpD lytA positive effect

Sequence

Protein


Download         Length: 216 a.a.        Molecular weight: 23229.65 Da        Isoelectric Point: 5.5171

>NTDB_id=188 SPR_RS04295 WP_000387330.1 849966..850616(+) (comEA/celA/cilE) [Streptococcus pneumoniae R6]
MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQAEVAAVSKDLVSEKEVNKEEKEEPLEQDLITVDVKG
AVKSPGIYDLPVGSRVNDAVQKAGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGLGTASSISKEKKVNLNKA
SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGGKTIEKLKDYVTVD

Nucleotide


Download         Length: 651 bp        

>NTDB_id=188 SPR_RS04295 WP_000387330.1 849966..850616(+) (comEA/celA/cilE) [Streptococcus pneumoniae R6]
ATGGAAGCAATTATCGAGAAAATCAAAGAGTATAAAATCATCGTCATCTGTACTGGTCTGGGCTTGCTTGTAGGAGGATT
TTTCCTGCTAAAACCAGCTCCACAAACACCTGTCAAAGAGACGAATTTGCAGGCTGAAGTCGCAGCTGTTTCCAAGGATT
TGGTATCCGAAAAGGAAGTGAACAAGGAAGAAAAGGAAGAACCCCTTGAACAAGATCTAATCACAGTAGATGTCAAAGGT
GCTGTCAAATCGCCAGGGATTTATGACTTGCCTGTAGGTAGTCGAGTCAATGATGCTGTTCAGAAGGCTGGTGGCTTGAC
AGAGCAAGCAGACAGCAAGTCGCTCAATCTAGCTCAGAAAGTTAGTGATGAGGCTCTGGTTTACGTTCCTACTAAGGGAG
AAGAAGCAGTTAGCCAACAGACTGGTTTGGGGACAGCTTCTTCAATAAGCAAGGAAAAGAAGGTCAATCTCAACAAGGCC
AGTCTGGAAGAACTCAAGCAGGTCAAGGGACTGGGAGGAAAACGAGCTCAGGACATTATCGACCATCGTGAGGCAAATGG
CAAGTTCAAGTCAGTAGACGAGCTCAAGAAGGTCTCTGGCATTGGTGGCAAAACAATAGAAAAGCTTAAAGACTATGTTA
CAGTGGATTAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(151-214)

SLBB domain (SLBB)

(76-126)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA/celA/cilE Streptococcus pneumoniae D39

100

100

1
  comEA/celA/cilE Streptococcus pneumoniae Rx1

100

100

1
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

97.222

100

0.972
  comEA/celA/cilE Streptococcus mitis NCTC 12261

96.296

100

0.963
  comEA/celA/cilE Streptococcus mitis SK321

90.278

100

0.903
  comEA Lactococcus lactis subsp. cremoris KW2

43.172

100

0.454
  Cj0011c Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

49.206

79.747

0.392
  comEA Streptococcus thermophilus LMD-9

39.035

98.701

0.385
  comEA Bacillus subtilis subsp. subtilis str. 168

41.579

87.963

0.366
  comEA Latilactobacillus sakei subsp. sakei 23K

33.476

100

0.361

Multiple sequence alignment    



References

[1] Yanni Liu et al. (2019) HtrA-mediated selective degradation of DNA uptake apparatus accelerates termination of pneumococcal transformation. Molecular Microbiology 112(4):1308-1325. [PMID: 31396996]
[2] Matthieu J Bergé et al. (2013) Midcell recruitment of the DNA uptake and virulence nuclease, EndA, for pneumococcal transformation. PLoS Pathogens 9(9):e1003596. [PMID: 24039578]
[3] Scott N Peterson et al. (2004) Identification of competence pheromone responsive genes in Streptococcus pneumoniae by use of DNA microarrays. Molecular Microbiology 51(4):1051-70. [PMID: 14763980]
[4] Mathieu Bergé et al. (2002) Uptake of transforming DNA in Gram-positive bacteria: a view from Streptococcus pneumoniae. Molecular Microbiology 45(2):411-21. [PMID: 12123453]
[5] E V Pestova et al. (1998) Isolation and characterization of three Streptococcus pneumoniae transformation-specific loci by use of a lacZ reporter insertion vector. Journal of Bacteriology 180(10):2701-10. [PMID: 9573156]