Detailed information NTG0000182

Experimentally validated

Overview

• Name: stkP
• Type: Regulator
• Locus tag: SPR_RS07820
• Genome accession: NC_003098
• Coordinates: 1556104..1558083 (-)
• Length: 659 a.a.
• NCBI ID: WP_000614552.1
• Uniprot ID: Q8DNS0
• Organism: Streptococcus pneumoniae R6
• Function: phosphorylate ComE Competence regulation

Function

StkP is involved in the regulation of cell wall synthesis and cell division/elongation in S. pneumoniae. A stkP mutant displays a strongly reduced natural transformability compared to wild type. StkP could phosphorylate ComE at Thr128 and the StkP/ComE pathway induced global changes in response to acidic stress.

Genomic Context

Location: 1551104..1563083

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SPR_RS07790 (spr1570)	-	1551199..1552473 (-)	1275	WP_000704388.1	hydroxymethylglutaryl-CoA reductase, degradative	-
SPR_RS07795 (spr1571)	-	1552473..1553645 (-)	1173	WP_000151964.1	hydroxymethylglutaryl-CoA synthase	-
SPR_RS07800 (spr1572)	-	1553776..1554024 (-)	249	WP_001818278.1	hypothetical protein	-
SPR_RS07805	-	1554092..1554718 (-)	627	Protein_1543	transposase family protein	-
SPR_RS07810 (spr1575)	-	1554780..1555229 (-)	450	WP_001856267.1	threonine/serine exporter family protein	-
SPR_RS07815 (spr1576)	-	1555231..1555989 (-)	759	WP_000392880.1	threonine/serine exporter family protein	-
SPR_RS07820 (spr1577)	stkP	1556104..1558083 (-)	1980	WP_000614552.1	serine/threonine-protein kinase StkP	Regulator
SPR_RS07825 (spr1578)	-	1558080..1558820 (-)	741	WP_000406247.1	Stp1/IreP family PP2C-type Ser/Thr phosphatase	-
SPR_RS07830 (spr1579)	rsmB	1558835..1560148 (-)	1314	WP_001808992.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
SPR_RS07835 (spr1580)	fmt	1560141..1561076 (-)	936	WP_000163696.1	methionyl-tRNA formyltransferase	-

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
stkP	comE	positive effect
comE	comB	positive effect
comB	comC/comC1	positive effect
comC/comC1	comD/comD1	positive effect
comD/comD1	comE	positive effect
comE	comW	positive effect
comW	comX/comX1	positive effect
comW	comX/comX2	positive effect
clpP	comW	negative effect
mecA	comW	negative effect
clpC	comW	negative effect
comE	comC/comC1	positive effect
comA	comC/comC1	positive effect
ciaH	comC/comC1	negative effect
ciaR	comC/comC1	negative effect
htrA	comC/comC1	negative effect
comE	comX/comX1	positive effect
comX/comX1	late competence genes	positive effect
clpE	comX/comX1	negative effect
clpP	comX/comX1	negative effect
comE	comA	positive effect
comE	comD/comD1	positive effect
comE	comX/comX2	positive effect
comX/comX2	late competence genes	positive effect
clpE	comX/comX2	negative effect
clpP	comX/comX2	negative effect
comE	comM	positive effect
comM	cbpD	negative effect
comE	comE	positive effect
ciaH	htrA	positive effect
ciaR	htrA	positive effect
htrA	comEA/celA/cilE	negative effect
htrA	comEC/celB	negative effect
comX/comX2	late competence genes	positive effect
comX/comX1	late competence genes	positive effect
cbpD	lytC	positive effect
cbpD	lytA	positive effect

Sequence

Protein

• Length: 659 a.a.
• Molecular weight: 72292.76 Da
• Isoelectric Point: 8.9204

>NTDB_id=182 SPR_RS07820 WP_000614552.1 1556104..1558083(-) (stkP) [Streptococcus pneumoniae R6]
MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVLRTNYQTDPIAVARFQREARAMADLDHPHIVRITDIG
EEDGQQYLAMEYVAGLDLKRYIKEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAVTIALQHFQNPLPSVIAENSSVP
QALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYNRRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIK
NPSQAVTEETYQPQAPKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANFEIGEEKTE
ASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSDVIAELKEKKVPDNLIKIEEEESNESEAGTV
LKQSLPEGTTYDLSKATQIVLTVAKKATTIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAG
TTYDVSKPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSVEGMVVEQSPRAGEKVDL
NKTRVKISIYKPKTTSATP

Nucleotide

• Length: 1980 bp

>NTDB_id=182 SPR_RS07820 WP_000614552.1 1556104..1558083(-) (stkP) [Streptococcus pneumoniae R6]
ATGATCCAAATCGGCAAGATTTTTGCCGGACGCTATCGGATTGTCAAACAGATTGGTCGAGGAGGCATGGCGGATGTCTA
CCTAGCCAAAGACTTAATCTTAGATGGGGAAGAAGTGGCAGTGAAGGTTCTGAGGACCAACTACCAGACGGACCCGATAG
CTGTAGCTCGTTTTCAGCGTGAAGCGAGAGCTATGGCAGATCTAGACCATCCTCATATCGTTCGGATAACAGATATTGGT
GAGGAAGACGGTCAACAGTATCTTGCAATGGAGTATGTTGCTGGACTAGACCTCAAACGCTATATCAAGGAACATTATCC
TCTTTCTAATGAAGAAGCAGTCCGTATCATGGGACAAATTCTCTTGGCTATGCGCTTGGCCCATACTCGAGGAATTGTTC
ACAGGGACTTGAAACCTCAAAATATCCTTTTGACACCAGATGGGACTGCCAAGGTCACAGACTTTGGGATTGCTGTAGCC
TTTGCAGAGACAAGTCTGACCCAGACTAACTCGATGTTGGGCTCAGTTCATTACTTGTCACCAGAGCAGGCGCGTGGTTC
GAAGGCGACTGTGCAGAGTGATATCTATGCCATGGGGATTATTTTCTATGAGATGTTGACAGGCCATATCCCTTATGACG
GGGATAGCGCGGTGACCATTGCCCTCCAGCATTTCCAGAACCCCCTGCCGTCCGTTATTGCAGAAAATTCATCTGTACCT
CAGGCTTTAGAAAATGTTATTATCAAGGCAACTGCTAAAAAGTTGACCAATCGCTATCGCTCGGTTTCAGAGATGTATGT
AGACTTGTCTAGTAGCTTGTCCTACAATCGTAGAAATGAAAGTAAGTTAATCTTTGATGAAACGAGCAAGGCAGATACCA
AGACCTTGCCGAAGGTTTCTCAGAGTACCTTGACATCTATTCCTAAGGTTCAAGCGCAGACAGAACACAAATCAATCAAA
AACCCAAGCCAGGCTGTGACAGAGGAAACTTACCAACCACAAGCACCGAAAAAACATAGATTTAAGATGCGTTACCTGAT
TTTGTTGGCCAGCCTTGTATTGGTGGCAGCTTCTCTTATTTGGATACTATCCAGAACTCCTGCAACCATTGCCATTCCAG
ATGTGGCAGGTCAGACAGTTGCAGAGGCCAAGGCAACGCTCAAAAAAGCCAATTTTGAGATTGGTGAGGAGAAGACAGAG
GCTAGTGAAAAGGTGGAAGAAGGGCGGATTATCCGTACAGATCCTGGCGCTGGAACTGGTCGAAAAGAAGGAACGAAAAT
TAATCTGGTTGTCTCATCAGGCAAACAATCCTTCCAAATTAGTAATTATGTCGGCCGGAAATCTTCTGATGTTATCGCGG
AATTAAAAGAGAAAAAAGTTCCAGATAATTTGATTAAAATTGAGGAAGAAGAGTCGAATGAAAGTGAGGCTGGAACGGTC
CTGAAGCAAAGTCTACCAGAAGGTACGACCTATGACTTGAGCAAGGCAACTCAAATTGTTTTGACAGTAGCTAAAAAAGC
TACGACGATTCAATTAGGGAACTATATTGGACGGAACTCTACAGAAGTAATCTCAGAACTCAAGCAGAAGAAGGTTCCTG
AGAATTTGATTAAGATAGAGGAAGAAGAGTCCAGCGAAAGCGAACCAGGAACGATTATGAAACAAAGTCCAGGTGCCGGA
ACGACTTATGATGTGAGTAAACCTACTCAAATTGTCTTGACAGTAGCTAAAAAAGTTACAAGTGTTGCCATGCCGAGTTA
CATTGGTTCCAGCTTGGAGTTTACTAAGAACAATTTGATTCAAATTGTTGGGATTAAGGAAGCTAATATAGAAGTTGTAG
AAGTGACGACAGCGCCTGCAGGTAGTGTAGAAGGCATGGTTGTTGAACAAAGTCCTAGAGCAGGTGAAAAGGTAGACCTA
AATAAGACTAGAGTCAAGATTTCAATCTACAAACCTAAAACAACTTCAGCTACTCCTTAA

Domains

Predicted by InterproScan.

• Protein kinase domain (Pkinase) (12-243)

• PASTA domain (PASTA) (440-505)

• PASTA domain (PASTA) (513-577)

• PASTA domain (PASTA) (582-651)

• PASTA domain (PASTA) (371-432)

3D structure

• PDB: 5NOD

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
stkP	Streptococcus pneumoniae D39	100	100	1
stkP	Streptococcus pneumoniae Rx1	99.848	100	0.998
stkP	Streptococcus pneumoniae TIGR4	99.545	100	0.995
pknB	Streptococcus mutans UA159	52.217	98.864	0.516
stkP/pknB	Streptococcus salivarius strain HSISS4	59.109	79.167	0.468

References

• [1]: Germán E Piñas et al. (2018) Crosstalk between the serine/threonine kinase StkP and the response regulator ComE controls the stress response and intracellular survival of Streptococcus pneumoniae. PLoS Pathogens 14(6):e1007118. [PMID: 29883472]
• [2]: Makoto Osaki et al. (2009) The StkP/PhpP signaling couple in Streptococcus pneumoniae: cellular organization and physiological characterization. Journal of Bacteriology 191(15):4943-50. [PMID: 19502404]
• [3]: Lenka Sasková et al. (2007) Eukaryotic-type serine/threonine protein kinase StkP is a global regulator of gene expression in Streptococcus pneumoniae. Journal of Bacteriology 189(11):4168-79. [PMID: 17416671]
• [4]: Jose Echenique et al. (2004) Protein serine/threonine kinase StkP positively controls virulence and competence in Streptococcus pneumoniae. Infection And Immunity 72(4):2434-7. [PMID: 15039376]