Detailed information NTG0000288

Experimentally validated

Overview

• Name: clpC
• Type: Regulator
• Locus tag: SPR_RS10185
• Genome accession: NC_003098
• Coordinates: 1992961..1995393 (-)
• Length: 810 a.a.
• NCBI ID: Protein_1974
• Uniprot ID: -
• Organism: Streptococcus pneumoniae R6
• Function: degradation of ComW Competence regulation

Function

ComW is a target of the Clp machinery and was shown to be degraded by ClpCP.

Genomic Context

Location: 1987961..2000393

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SPR_RS10170 (spr1996)	-	1989728..1990255 (-)	528	WP_000241918.1	isoprenylcysteine carboxyl methyltransferase family protein	-
SPR_RS10175 (spr1997)	hk06	1990338..1991669 (-)	1332	WP_000594665.1	two-component system sensor histidine kinase HK06	-
SPR_RS10180 (spr1998)	rr06	1991666..1992319 (-)	654	WP_001024075.1	two-component system response regulator RR06	-
SPR_RS10185 (spr1999)	clpC	1992961..1995393 (-)	2433	Protein_1974	ATP-dependent Clp protease ATP-binding subunit	Regulator
SPR_RS10190 (spr2001)	-	1995395..1995853 (-)	459	WP_001211279.1	CtsR family transcriptional regulator	-
SPR_RS10195 (spr2002)	-	1995972..1996700 (-)	729	WP_000136224.1	ABC transporter ATP-binding protein	-
SPR_RS10200 (spr2003)	-	1996700..1997707 (-)	1008	WP_000754546.1	ABC transporter substrate-binding protein	-
SPR_RS10205 (spr2004)	-	1997745..1998503 (-)	759	WP_001245132.1	ABC transporter permease	-
SPR_RS10210 (spr2005)	-	1998466..1998756 (-)	291	WP_000647661.1	thiamine-binding protein	-
SPR_RS10215 (spr2006)	cbpD	1999012..2000358 (-)	1347	WP_000698573.1	choline binding-anchored murein hydrolase CbpD	Auxiliary factor

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
clpC	comW	negative effect
comW	comX/comX1	positive effect
comX/comX1	late competence genes	positive effect
comX/comX2	late competence genes	positive effect
comX/comX2	late competence genes	positive effect
clpE	comX/comX2	negative effect
clpP	comX/comX2	negative effect
comW	comX/comX2	positive effect
comE	comX/comX2	positive effect
comX/comX1	late competence genes	positive effect
comE	comX/comX1	positive effect
clpE	comX/comX1	negative effect
clpP	comX/comX1	negative effect
clpP	comW	negative effect
mecA	comW	negative effect
comE	comW	positive effect
comE	comB	positive effect
comE	comC/comC1	positive effect
comE	comA	positive effect
comE	comD/comD1	positive effect
comE	comM	positive effect
comE	comE	positive effect
comD/comD1	comE	positive effect
stkP	comE	positive effect
comB	comC/comC1	positive effect
comC/comC1	comD/comD1	positive effect
comA	comC/comC1	positive effect
ciaH	comC/comC1	negative effect
ciaR	comC/comC1	negative effect
htrA	comC/comC1	negative effect
comM	cbpD	negative effect
ciaH	htrA	positive effect
ciaR	htrA	positive effect
htrA	comEA/celA/cilE	negative effect
htrA	comEC/celB	negative effect
cbpD	lytC	positive effect
cbpD	lytA	positive effect

Sequence

Protein

• Length: 810 a.a.
• Molecular weight: Da
• Isoelectric Point:

>NTDB_id=288 SPR_RS10185 Protein_1974 1992961..1995393(-) (clpC) [Streptococcus pneumoniae R6]
None

Nucleotide

• Length: 2433 bp

>NTDB_id=288 SPR_RS10185 Protein_1974 1992961..1995393(-) (clpC) [Streptococcus pneumoniae R6]
ATGAACTATTCAAAAGCATTGAATGAATGTATCGAAAGTGCCTACATGGTTGCTGGACATTTTGGAGCTCGTTATCTAGA
GTCTTGGCACTTGTTGATTGCCATGTCTAATCACAGTTATAGTGTAGCAGGGGCAACTTTAAATGATTATCCGTATGAGA
TGGACCGTTTAGAAGAGGTGGCTTTGGAACTGACTGAAACGGACTATAGCCAGGATGAAACTTTTACGGAATTGCCGTTC
TCCCGTCGTTTGCAGATTCTTTTTGACGAAGCAGAGTATGTAGCGTCAGTGGTCCATGCTAAGGTGCTAGGTACAGAGCA
CGTCCTCTATGCGATTTTGCATGATGGCAATGCCTTGGCGACTCGTATCTTGGAGAGGGCTGGTTTTTCTTATGAAGACA
AGAAAGATCAGGTCAAGATTGCTGCTCTTCGTCGAAATTTAGAAGAACGGGCAGGCTGGACTCGTGAAGATCTCAAGGCT
TTACGCCAACGCCATCGTACAGTAGCTGACAAGCAAAATTCTATGGCCAATATGATGGGCATGCCGCAGACTCCTAGTGG
TGGTCTCGAGGACTATACGCATGATTTGACAGAGCAAGCGCGTTCTGGCAAGTTAGAACCAGTCATCGGTCGGGACAAGG
AAATCTCACGTATGATTCAAATCTTAAGCCGGAAGACTAAGAACAACCCTGTCTTGGTTGGGGATGCTGGTGTCGGGAAA
ACAGCTCTGGCGCTTGGTCTTGCTCAGCGTATTGCCAGTGGTGACGTGCCTGCGGAAATGGCTAAGATGCGCGTGTTAGA
ACTTGATTTGATGAATGTCGTTGCAGGGACACGCTTCCGTGGTGACTTTGAAGAACGCATGAATAATATCATCAAGGATA
TTGAAGAAGATGGCCAAGTCATCCTCTTTATCGATGAACTCCACACCATCATGGGTTCTGGTAGCGGGATTGATTCGACT
CTGGATGCGGCCAATATCTTGAAACCAGCCTTGGCGCGTGGAACTTTGAGAACGGTTGGTGCCACCACTCAGGAAGAATA
TCAAAAACATATCGAAAAAGATGCGGCACTTTCTCGTCGTTTCGCTAAAGTGACGATTGAAGAACCAAGTGTGGCAGATA
GTATGACTATTTTACAAGGTTTGAAGGCGACTTATGAGAAACATCACCGTGTACAAATCACAGATGAAGCGGTTGAAACA
GCGGTTAAGATGGCTCATCGTTATTTAACCAGTCGTCACTTGCCAGACTCTGCTATCGACCTCTTGGACGAAGCAGCAGC
AACAGTGCAAAATAAGGCAAAGCATGTAAAAGCAGACGATTCAGATTTGAGTCCAGCTGACAAGGCCCTGATGGATGGCA
AGTGGAAACAGGCAGCCCAGCTAATCGCAAAAGAAGAGGAAGTACCTGTCTACAAAGACTTGGTGACAGAGTCTGATATT
TTGACCACCTTGAGTCGCTTGTCAGGAATCCCAGTTCAAAAACTGACTCAGACTGACGCTAAGAAATATCTAAATCTTGA
AGCAGAACTCCATAAACGGGTTATCGGTCAAGATCAAGCTGTTTCAAGTATTAGTCGTGCGATTCGCCGTAATCAGTCAG
GAATTCGCAGTCACAAGCGTCCGATCGGTTCCTTTATGTTCCTAGGACCCACAGGTGTCGGTAAGACCGAGTTGGCCAAG
GCTTTGGCAGAAGTTCTCTTTGATGACGAATCAGCCCTTATCCGCTTTGATATGAGTGAGTATATGGAGAAATTCGCAGC
CAGCCGTCTAAATGGAGCTCCTCCAGGCTATGTGGGTTACGAAGAAGGTGGGGAGTTGACAGAGAAGGTTCGCAATAAAC
CCTATTCCGTTCTCCTCTTTGATGAGGTAGAGAAGGCCCACCCAGATATCTTTAATGTTCTCTTGCAGGTTCTGGATGAC
GGTGTCTTGACAGATAGCAAGGGACGCAAGGTCGATTTTTCAAATACCATTATCATTATGACATCGAATCTAGGTGCGAC
TGCCCTTCGTGATGATAAGACTGTTGGTTTTGGGGCTAAGGATATTCGTTTTGACCAGGAAAATATGGAAAAACGCATGT
TTGAAGAACTGAAAAAAGCTTATAGACCGGAATTCATCAACCGTATTGATGAGAAGGTGGTCTTCCATAGCCTATCTAGT
GACCATATGCAGGAAGTGGTGAAGATTATGGTCAAGCCTTTAGTGGCAAGTTTGGCTGAAAAAGGCATTGACTTGAAATT
ACAAGCTTCAGCTCTGAAATTGTTAGCAAATCAAGGATATGACCCATAGATGGGAGCTCGCCCACTTCGCAGAACCCTGC
AAACAGAAGTGGAGGACAAGTTGGCAGAACTTCTTCTTAAGGGAGATTTAGTGGCAGGCAGCATACTTAAGATTGGTGTC
AAAGCAGGCCAGTTAAAATTTGATATTGCATAA

Domains

No domain identified.

References

• [1]: Andrew Piotrowski et al. (2009) Competence for genetic transformation in Streptococcus pneumoniae: termination of activity of the alternative sigma factor ComX is independent of proteolysis of ComX and ComW. Journal of Bacteriology 191(10):3359-66. [PMID: 19286798]