Detailed information    

experimental Experimentally validated

Overview

Name   comEA   Type   Machinery gene
Locus tag   KW2_RS08725 Genome accession   NC_022369
Coordinates   1847667..1848323 (-) Length   218 a.a.
NCBI ID   WP_021037687.1    Uniprot ID   -
Organism   Lactococcus lactis subsp. cremoris KW2     
Function   dsDNA binding to the cell surface   
DNA binding and uptake

Function

ComX induce the expression of late competence genes.


Genomic Context

Location: 1842667..1853323
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KW2_RS08700 (kw2_1746) atpF 1842737..1843243 (-) 507 WP_010906128.1 F0F1 ATP synthase subunit B -
  KW2_RS08705 (kw2_1747) atpB 1843258..1843971 (-) 714 WP_004255255.1 F0F1 ATP synthase subunit A -
  KW2_RS08710 (kw2_1748) - 1844018..1844233 (-) 216 WP_004255250.1 F0F1 ATP synthase subunit C -
  KW2_RS08715 (kw2_1749) - 1844416..1845192 (-) 777 WP_014572211.1 alpha/beta hydrolase -
  KW2_RS08720 (kw2_1750) comEC 1845476..1847686 (-) 2211 WP_021037686.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  KW2_RS08725 (kw2_1751) comEA 1847667..1848323 (-) 657 WP_021037687.1 helix-hairpin-helix domain-containing protein Machinery gene
  KW2_RS08730 (kw2_1752) - 1848381..1849742 (-) 1362 WP_021037688.1 ABC transporter permease -
  KW2_RS08735 (kw2_1753) - 1849739..1850671 (-) 933 WP_021037689.1 ABC transporter ATP-binding protein -
  KW2_RS08740 (kw2_1754) - 1850777..1851175 (-) 399 WP_021037690.1 hypothetical protein -
  KW2_RS08745 (kw2_1755) - 1851293..1851853 (-) 561 WP_021037691.1 GNAT family N-acetyltransferase -
  KW2_RS08750 (kw2_1756) - 1852027..1853205 (-) 1179 WP_021037692.1 SLC13 family permease -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  comX comEA positive effect
  comX comGG positive effect
  comX ssbB positive effect
  comX comGD positive effect
  comX dprA positive effect
  comX comFA positive effect
  comX coiA positive effect
  comX comGB positive effect
  comX recA positive effect
  comX comFC positive effect
  comX comGE positive effect
  comX comGF positive effect
  comX comEC positive effect
  comX comGA positive effect
  comX comGC positive effect
  mecA comX negative effect
  clpC comX negative effect
  clpP comX negative effect

Sequence

Protein


Download         Length: 218 a.a.        Molecular weight: 23739.89 Da        Isoelectric Point: 5.1730

>NTDB_id=595 KW2_RS08725 WP_021037687.1 1847667..1848323(-) (comEA) [Lactococcus lactis subsp. cremoris KW2]
MEKILDKLKEYWKIMLLVVCALIAGGIFYIFTNSPKPAETLSVENLSSSSTKSSVSKFNSSSSEKNKNEIMVDLKGAVAK
PNVYQISSDERLVDLIRQAGGFTDQADQKSINLSAKLKDEEVIYVPKVGESSSSESTDSPTGSSVSNQISTTSGPKVNIN
KADLTELQKLTGIGQKKAQDIIDFRMKNGDFKSIEDLGKVSGFGDKTLEKLKDEISID

Nucleotide


Download         Length: 657 bp        

>NTDB_id=595 KW2_RS08725 WP_021037687.1 1847667..1848323(-) (comEA) [Lactococcus lactis subsp. cremoris KW2]
ATGGAAAAAATTTTAGATAAATTAAAAGAATACTGGAAAATAATGCTTTTAGTTGTTTGTGCACTTATTGCTGGTGGAAT
TTTTTATATTTTCACCAATTCACCAAAGCCAGCTGAAACTCTGTCAGTAGAAAATTTGAGCTCTAGTTCTACCAAAAGCT
CTGTCAGTAAATTTAATAGTAGCAGTAGTGAAAAAAATAAAAATGAAATTATGGTTGATTTGAAAGGAGCAGTTGCAAAA
CCTAATGTATACCAAATTTCGTCAGATGAGCGCCTTGTTGATTTAATTAGGCAAGCGGGAGGATTTACTGACCAAGCAGA
CCAAAAATCAATCAATCTGTCAGCGAAACTTAAAGATGAAGAAGTGATTTATGTACCAAAAGTTGGGGAAAGTTCAAGTT
CAGAAAGTACTGACAGTCCTACTGGTAGCTCTGTCAGTAATCAAATTTCAACGACAAGTGGTCCAAAAGTAAATATCAAT
AAAGCAGACCTGACAGAATTACAAAAACTGACTGGAATTGGTCAAAAAAAAGCGCAAGATATTATTGATTTTCGCATGAA
AAATGGTGATTTTAAATCAATAGAGGATTTGGGTAAAGTATCTGGCTTTGGGGATAAAACATTAGAAAAACTGAAAGATG
AGATTTCTATTGATTAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(154-215)

SLBB domain (SLBB)

(71-126)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA/celA/cilE Streptococcus pneumoniae R6

43.172

100

0.454
  comEA/celA/cilE Streptococcus pneumoniae D39

43.172

100

0.454
  comEA/celA/cilE Streptococcus pneumoniae Rx1

43.172

100

0.454
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

42.291

100

0.44
  comEA/celA/cilE Streptococcus mitis SK321

41.778

100

0.435
  comEA/celA/cilE Streptococcus mitis NCTC 12261

40.889

100

0.426
  Cj0011c Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

53.226

78.481

0.418
  comEA Latilactobacillus sakei subsp. sakei 23K

38.095

100

0.404
  comEA Staphylococcus aureus MW2

37.037

99.083

0.367

Multiple sequence alignment    



References

[1] Blandine David et al. (2017) Natural DNA Transformation Is Functional in Lactococcus lactis subsp. cremoris KW2. Applied And Environmental Microbiology 83(16):e01074-17. [PMID: 28625996]