Detailed information NTG0000600

Experimentally validated

Overview

• Name: recA
• Type: Machinery gene
• Locus tag: KW2_RS01765
• Genome accession: NC_022369
• Coordinates: 354969..356132 (+)
• Length: 387 a.a.
• NCBI ID: WP_011675413.1
• Uniprot ID: Q031W5
• Organism: Lactococcus lactis subsp. cremoris KW2
• Function: homologous recombination Homologous recombination

Function

ComX induce the expression of late competence genes.

Genomic Context

Location: 349969..361132

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KW2_RS01750 (kw2_0354)	era	351205..352116 (+)	912	WP_003131632.1	GTPase Era	-
KW2_RS01755 (kw2_0355)	asnB	352214..353806 (+)	1593	WP_021036706.1	asparagine synthase B	-
KW2_RS01760 (kw2_0356)	mutM	353998..354816 (+)	819	WP_021036707.1	DNA-formamidopyrimidine glycosylase	-
KW2_RS01765 (kw2_0357)	recA	354969..356132 (+)	1164	WP_011675413.1	recombinase RecA	Machinery gene
KW2_RS01770 (kw2_0358)	-	356147..357529 (-)	1383	WP_021036708.1	amino acid permease	-
KW2_RS01775 (kw2_0359)	-	357601..358980 (-)	1380	WP_021036709.1	amino acid permease	-
KW2_RS01780 (kw2_0360)	-	359127..359507 (+)	381	WP_021036710.1	YlbF family regulator	-
KW2_RS01785 (kw2_0361)	-	359494..359805 (+)	312	WP_011675417.1	DUF2129 domain-containing protein	-
KW2_RS01790 (kw2_0362)	-	359856..360548 (+)	693	WP_235182443.1	dithiol-disulfide isomerase	-

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
comX	recA	positive effect
comX	comGG	positive effect
comX	comEA	positive effect
comX	ssbB	positive effect
comX	comGD	positive effect
comX	dprA	positive effect
comX	comFA	positive effect
comX	coiA	positive effect
comX	comGB	positive effect
comX	comFC	positive effect
comX	comGE	positive effect
comX	comGF	positive effect
comX	comEC	positive effect
comX	comGA	positive effect
comX	comGC	positive effect
mecA	comX	negative effect
clpC	comX	negative effect
clpP	comX	negative effect

Sequence

Protein

• Length: 387 a.a.
• Molecular weight: 41380.10 Da
• Isoelectric Point: 4.9920

>NTDB_id=600 KW2_RS01765 WP_011675413.1 354969..356132(+) (recA) [Lactococcus lactis subsp. cremoris KW2]
MATKKKTNFDDITKKYGAERDKALADALALIEKDFGKGSLMRLGEAANQKVSVVSSGSLALDIALGAGGYPKGRIVEIYG
PESSGKTTVALHAVAAVQKEGGIAAFIDAENALDPEYAKALGVNIDELLLSQPDYGEQGLQIAEKLITSGAVDLVVIDSV
AALVPKAEIDGEIGDSSVGLQARMMSQAMRKLAGHINKTKTTAIFINQLREKVGVMFGSPETTPGGRALKFYASVRLDVR
GSTKIEEGSGDNKTQIGKITKIKVVKNKVAPPFKVALVDIMFGEGISSTGELLNIAVEEGIIKKSGAWFAYNDEKIGQGA
EKAKNYLKEHQDVFDEIDHKVRAAHGLLDDSEVAETEEETTASKTKAKAKKEEKAVETEEIELELED

Nucleotide

• Length: 1164 bp

>NTDB_id=600 KW2_RS01765 WP_011675413.1 354969..356132(+) (recA) [Lactococcus lactis subsp. cremoris KW2]
TTGGCAACAAAGAAAAAAACAAATTTTGATGATATTACCAAAAAATATGGCGCTGAGCGTGATAAAGCATTGGCTGATGC
TCTTGCTCTGATTGAAAAAGACTTTGGGAAAGGTTCACTCATGCGTCTTGGTGAAGCAGCTAACCAAAAAGTATCAGTTG
TAAGTTCAGGTTCTCTTGCGCTTGATATTGCACTTGGTGCAGGTGGTTACCCTAAAGGTCGTATCGTTGAAATCTACGGA
CCAGAATCTTCTGGTAAAACAACAGTCGCTCTTCATGCCGTTGCAGCGGTTCAAAAAGAAGGTGGAATTGCAGCCTTTAT
CGATGCTGAAAATGCTCTTGACCCAGAATATGCAAAAGCACTCGGTGTAAATATTGATGAACTTTTGCTCTCACAACCTG
ACTATGGTGAACAAGGACTTCAAATTGCTGAAAAATTGATTACTTCTGGAGCGGTTGACTTGGTCGTTATCGACTCAGTT
GCAGCCTTGGTACCAAAGGCCGAAATTGATGGCGAAATCGGAGACAGTTCAGTTGGTCTTCAAGCACGTATGATGTCACA
AGCTATGCGTAAACTTGCAGGACACATCAACAAAACAAAAACAACAGCGATTTTCATCAACCAATTGCGTGAAAAAGTTG
GAGTGATGTTTGGTTCACCAGAAACAACTCCTGGTGGTCGTGCCTTGAAATTTTACGCCTCAGTTCGTCTTGACGTACGT
GGTTCAACAAAAATTGAAGAAGGTTCTGGAGATAACAAAACTCAAATTGGTAAAATCACCAAAATCAAAGTCGTTAAAAA
TAAAGTTGCACCACCATTTAAGGTTGCGCTTGTTGATATCATGTTTGGTGAAGGAATTTCAAGTACAGGTGAGCTTTTAA
ATATTGCTGTTGAAGAAGGCATTATTAAGAAATCTGGTGCTTGGTTTGCTTATAATGATGAGAAGATTGGTCAAGGGGCA
GAAAAAGCTAAAAACTACCTCAAAGAGCATCAAGATGTTTTTGATGAAATAGACCACAAAGTTCGGGCAGCTCATGGACT
TCTTGATGATTCAGAAGTAGCAGAAACTGAAGAAGAAACTACAGCTTCAAAAACTAAAGCAAAAGCTAAAAAAGAAGAAA
AAGCAGTAGAAACAGAAGAAATCGAACTTGAACTTGAAGATTAA

Domains

Predicted by InterProScan.

• recA bacterial DNA recombination protein (RecA_N) (22-287)

3D structure

• AlphaFold DB: Q031W5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
recA	Streptococcus pneumoniae R36A	74.805	99.483	0.744
recA	Streptococcus pneumoniae D39	74.805	99.483	0.744
recA	Streptococcus pneumoniae Rx1	74.805	99.483	0.744
recA	Streptococcus pneumoniae R6	74.805	99.483	0.744
recA	Streptococcus pneumoniae TIGR4	74.805	99.483	0.744
recA	Streptococcus mitis SK321	73.834	99.742	0.736
recA	Streptococcus mitis NCTC 12261	73.834	99.742	0.736
recA	Streptococcus pyogenes NZ131	78.47	93.386	0.733
recA	Streptococcus thermophilus LMD-9	77.62	93.14	0.723
recA	Streptococcus mutans UA159	72.208	99.483	0.718
recA	Streptococcus thermophilus LMG 18311	77.62	91.927	0.714
recA	Latilactobacillus sakei subsp. sakei 23K	70.359	94.085	0.662
recA	Staphylococcus aureus strain ATCC 12600	65.559	95.389	0.625
recA	Bacillus subtilis subsp. subtilis str. 168	65.758	94.828	0.624
recA	Riemerella anatipestifer ATCC 11845 = DSM 15868	60.486	95.918	0.58
recA	Neisseria gonorrhoeae strain FA1090	60.303	94.828	0.572
recA	Neisseria gonorrhoeae MS11	60.303	94.828	0.572
recA	Ralstonia pseudosolanacearum GMI1000	60.303	93.75	0.565
recA	Vibrio cholerae O1 biovar El Tor strain E7946	60.991	91.243	0.556
recA	Vibrio cholerae strain A1552	60.991	91.243	0.556
recA	Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819	57.447	95.918	0.551
recA	Helicobacter pylori 26695	58.055	94.813	0.55
recA	Helicobacter pylori strain NCTC11637	58.055	94.813	0.55
recA	Acinetobacter baylyi ADP1	59.443	92.55	0.55
recA	Acinetobacter baumannii D1279779	58.824	92.55	0.544
recA	Pseudomonas stutzeri DSM 10701	58.025	93.372	0.542
recA	Acinetobacter nosocomialis M2	58.514	92.286	0.54
recA	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	57.817	93.388	0.54
recA	Glaesserella parasuis strain SC1401	57.558	92.473	0.532

References

• [1]: Blandine David et al. (2017) Natural DNA Transformation Is Functional in Lactococcus lactis subsp. cremoris KW2. Applied And Environmental Microbiology 83(16):e01074-17. [PMID: 28625996]