Detailed information    

experimental Experimentally validated

Overview

Name   recA   Type   Machinery gene
Locus tag   Spy49_1753c Genome accession   CP000829
Coordinates   1744405..1745541 (-) Length   378 a.a.
NCBI ID   ACI62003.1    Uniprot ID   -
Organism   Streptococcus pyogenes NZ131     
Function   homologous recombination   
Homologous recombination

Function

Resistance to UV and the ability to accept linear DNA transformation by Rec11 (a recA mutant) were greatly decreased, confirming its RecA phenotype.


Genomic Context

Location: 1739405..1750541
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Spy49_1748c - 1740628..1742187 (-) 1560 ACI61998.1 hypothetical protein -
  Spy49_1749c - 1742600..1742905 (-) 306 ACI61999.1 Conserved hypothetical protein -
  Spy49_1750c - 1742917..1743336 (-) 420 ACI62000.1 Putative Holliday junction resolvase -
  Spy49_1751c - 1743333..1743602 (-) 270 ACI62001.1 UPF0297 protein SPy2114 -
  Spy49_1752c - 1743716..1744114 (-) 399 ACI62002.1 Conserved hypothetical protein -
  Spy49_1753c recA 1744405..1745541 (-) 1137 ACI62003.1 RecA protein Machinery gene
  Spy49_1754c cinA 1745629..1746900 (-) 1272 ACI62004.1 CinA-like protein Machinery gene
  Spy49_1755c tag 1746969..1747529 (-) 561 ACI62005.1 DNA-3-methyladenine glycosylase -
  Spy49_1756c ruvA 1747539..1748135 (-) 597 ACI62006.1 Holliday junction DNA helicase ruvA -
  Spy49_1757c lmrP 1748137..1749357 (-) 1221 ACI62007.1 Putative integral membrane protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 40615.23 Da        Isoelectric Point: 4.9004

>NTDB_id=453 Spy49_1753c ACI62003.1 1744405..1745541(-) (recA) [Streptococcus pyogenes NZ131]
MAKKLKKNEEITKKFGDERRKALDDALKNIEKDFGKGAVMRLGERAEQKVQVMSSGSLALDIALGAGGYPKGRIIEIYGP
ESSGKTTVALHAVAQAQKEGGIAAFIDAEHALDPAYAAALGVNIDELLLAQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLSASINKTKTIAIFINQLREKVGVMFGNPETTPGGRALKFYASVRLDVRG
TTQIKGTGDQKDSSIGKETKIKVVKNKVAPPFKVAEVEIMYGEGISRTGELVKIASDLDIIQKAGAWFSYNGEKIGQGSE
NAKRYLADHPQLFDEIDRKVRVKFGLLEESEEESAMAVASEETDDLALDLDNGIEIED

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=453 Spy49_1753c ACI62003.1 1744405..1745541(-) (recA) [Streptococcus pyogenes NZ131]
TTGGCAAAAAAATTAAAAAAGAATGAAGAAATAACTAAGAAGTTTGGTGACGAACGTCGCAAAGCCCTTGATGATGCTTT
GAAAAATATTGAAAAAGATTTTGGTAAGGGCGCAGTGATGCGATTAGGAGAACGTGCAGAGCAAAAAGTTCAGGTTATGA
GTTCAGGAAGTCTAGCTCTTGATATTGCGCTTGGAGCTGGTGGTTATCCTAAAGGACGTATCATCGAAATCTATGGTCCA
GAGTCTTCCGGTAAAACGACTGTGGCTTTACATGCTGTAGCACAAGCTCAAAAAGAAGGTGGAATCGCAGCCTTTATCGA
TGCCGAGCATGCGCTTGATCCAGCTTATGCTGCTGCGCTTGGGGTTAATATTGATGAACTTCTCTTGGCTCAACCAGATT
CTGGAGAACAAGGACTTGAAATTGCAGGTAAATTGATTGATTCTGGTGCGGTTGACCTGGTTGTTGTCGATTCAGTAGCA
GCTTTAGTGCCACGTGCTGAAATTGATGGTGATATTGGCGATAGCCATGTCGGATTGCAAGCACGTATGATGAGTCAGGC
CATGCGTAAATTATCAGCTTCTATTAATAAAACAAAAACTATCGCTATCTTTATTAACCAATTGCGTGAAAAAGTTGGTG
TGATGTTTGGAAATCCTGAAACAACACCAGGTGGTCGAGCTTTGAAATTCTATGCTTCTGTTCGGCTGGATGTGCGTGGA
ACAACTCAAATTAAAGGAACTGGTGACCAAAAAGATAGCAGCATTGGTAAGGAGACCAAAATCAAGGTTGTTAAAAACAA
GGTCGCTCCGCCATTTAAGGTAGCAGAAGTTGAAATCATGTATGGGGAAGGTATTTCTCGTACAGGGGAGCTTGTGAAAA
TTGCTTCTGATTTGGACATTATCCAAAAAGCAGGTGCTTGGTTCTCTTATAATGGTGAGAAGATTGGCCAAGGTTCTGAA
AATGCTAAGCGTTATTTGGCCGATCATCCACAATTGTTTGATGAAATCGACCGTAAAGTACGTGTTAAATTTGGTTTGCT
TGAAGAAAGCGAAGAAGAATCTGCTATGGCAGTAGCATCAGAAGAAACCGATGATCTTGCTTTAGATTTAGATAATGGTA
TTGAAATTGAAGATTAA

Domains

Predicted by InterproScan.

recA bacterial DNA recombination protein (RecA)

(21-286)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recA Streptococcus mutans UA159

87.206

100

0.884
  recA Streptococcus mitis NCTC 12261

85.079

100

0.86
  recA Streptococcus pneumoniae TIGR4

84.156

100

0.857
  recA Streptococcus pneumoniae Rx1

84.156

100

0.857
  recA Streptococcus pneumoniae D39

84.156

100

0.857
  recA Streptococcus pneumoniae R6

84.156

100

0.857
  recA Streptococcus mitis SK321

85.039

100

0.857
  recA Lactococcus lactis subsp. cremoris KW2

78.47

93.386

0.733
  recA Latilactobacillus sakei subsp. sakei 23K

71.429

92.676

0.662
  recA Bacillus subtilis subsp. subtilis str. 168

67.683

94.253

0.638
  recA Riemerella anatipestifer ATCC 11845 = DSM 15868

61.721

98.251

0.606
  recA Acinetobacter baumannii D1279779

58.924

100

0.596
  recA Neisseria gonorrhoeae strain FA1090

61.963

93.678

0.58
  recA Neisseria gonorrhoeae MS11

61.963

93.678

0.58
  recA Neisseria gonorrhoeae MS11

61.963

93.678

0.58
  recA Acinetobacter baylyi ADP1

62.154

93.123

0.579
  recA Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

58.788

96.21

0.566
  recA Vibrio cholerae strain A1552

60.615

91.808

0.556
  recA Vibrio cholerae O1 biovar El Tor strain E7946

60.615

91.808

0.556
  recA Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

62.229

88.981

0.554
  recA Helicobacter pylori 26695

57.879

95.101

0.55
  recA Helicobacter pylori strain NCTC11637

57.879

95.101

0.55
  recA Pseudomonas stutzeri DSM 10701

57.927

94.524

0.548
  recA Ralstonia pseudosolanacearum GMI1000

58.537

93.182

0.545
  recA Glaesserella parasuis strain SC1401

59.412

91.398

0.543

Multiple sequence alignment    



References

[1] L Tao et al. (1995) Construction of a Streptococcus pyogenes recA mutant via insertional inactivation, and cloning and sequencing of the complete recA gene. Gene 162(1):59-62. [PMID: 7557418]