Detailed information    

experimental Experimentally validated

Overview

Name   recA   Type   Machinery gene
Locus tag   PSJM300_05545 Genome accession   CP003725
Coordinates   1208801..1209844 (+) Length   347 a.a.
NCBI ID   AFN77183.1    Uniprot ID   -
Organism   Pseudomonas stutzeri DSM 10701     
Function   homologous recombination   
Homologous recombination

Function

The recA mutant strain was found to be completely deficient in natural transformation with chromosomal DNA, due to the impairment in homologous recombination.


Genomic Context

Location: 1203801..1214844
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSJM300_05525 - 1204048..1204239 (+) 192 AFN77179.1 hypothetical protein -
  PSJM300_05530 - 1204312..1204635 (-) 324 AFN77180.1 ferredoxin I -
  PSJM300_05535 - 1205044..1207623 (-) 2580 AFN77181.1 DNA mismatch repair protein MutS -
  PSJM300_05540 - 1208216..1208716 (+) 501 AFN77182.1 CinA, C-terminal protein -
  PSJM300_05545 recA 1208801..1209844 (+) 1044 AFN77183.1 recombinase A Machinery gene
  PSJM300_05550 recX 1209849..1210307 (+) 459 AFN77184.1 recombination regulator RecX -
  PSJM300_05555 - 1210452..1211918 (-) 1467 AFN77185.1 phosphate ABC transporter periplasmic protein -
  PSJM300_05560 - 1212125..1213057 (+) 933 AFN77186.1 methyltransferase -
  PSJM300_05565 - 1213097..1213918 (-) 822 AFN77187.1 nucleoside-binding outer membrane protein -
  PSJM300_05570 - 1214168..1214299 (-) 132 AFN77188.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 37008.41 Da        Isoelectric Point: 5.8713

>NTDB_id=1205 PSJM300_05545 AFN77183.1 1208801..1209844(+) (recA) [Pseudomonas stutzeri DSM 10701]
MDENKKRALAAALGQIEKQFGKGAVMRMGDHDRQAIPSISTGSLGLDIALGIGGLPKGRIVEIYGPESSGKTTLTLSVIA
AAQRMGSTCAFVDAEHALDPDYAGKLGVNVDDLLVSQPDTGEQALEITDMLVRSNAVDVIIVDSVAALVPKAEIEGEMGD
AHVGLQARLMSQALRKITGNIKNANCLVIFINQIRMKIGVMFGSPETTTGGNALKFYASVRLDIRRTGAVKEGDEVVGSE
TRVKVVKNKVAPPFRQAEFQILYGKGIYRNGEIIDLGVQQGLVEKSGAWYAYKGSKIGQGKANAAKYLEENPEIGQEIEQ
QIRAKLLVTSANTKATPVAEDLADADI

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1205 PSJM300_05545 AFN77183.1 1208801..1209844(+) (recA) [Pseudomonas stutzeri DSM 10701]
ATGGACGAGAACAAGAAGCGTGCCTTGGCCGCTGCCCTGGGCCAGATCGAAAAGCAGTTCGGCAAAGGCGCGGTCATGCG
CATGGGCGATCATGATCGCCAGGCGATCCCTTCTATTTCCACCGGCTCCCTGGGGCTGGATATCGCCCTCGGCATAGGCG
GTCTGCCGAAAGGCCGGATCGTCGAAATCTACGGCCCGGAATCTTCGGGTAAAACCACCCTGACCCTCTCGGTCATCGCC
GCCGCCCAGAGAATGGGCTCCACCTGCGCCTTCGTCGATGCCGAGCATGCGCTGGACCCCGATTACGCCGGCAAGCTGGG
CGTCAACGTCGACGACCTGCTGGTGTCGCAGCCCGACACGGGCGAGCAGGCGCTGGAAATCACCGACATGCTGGTGCGCT
CCAATGCAGTGGACGTGATCATCGTCGACTCCGTGGCAGCGCTGGTGCCCAAGGCGGAAATCGAAGGCGAGATGGGCGAT
GCCCACGTCGGTCTGCAGGCCCGCCTGATGTCCCAGGCGCTGCGCAAGATCACCGGCAACATCAAGAACGCCAACTGCCT
GGTGATCTTCATCAACCAGATCCGCATGAAGATCGGCGTGATGTTCGGTAGCCCGGAAACCACCACCGGCGGTAACGCGC
TGAAGTTCTACGCTTCGGTCCGCCTGGACATCCGCCGTACTGGCGCGGTGAAGGAAGGCGACGAAGTGGTGGGCAGCGAA
ACCCGCGTCAAGGTCGTCAAGAACAAGGTGGCCCCGCCGTTCCGCCAGGCCGAGTTCCAGATCCTCTACGGCAAGGGCAT
CTACCGCAACGGCGAGATCATCGATCTCGGCGTGCAGCAGGGCCTGGTGGAGAAATCCGGTGCCTGGTACGCCTACAAGG
GCAGCAAGATCGGCCAGGGCAAGGCCAATGCGGCCAAGTATCTGGAAGAGAACCCGGAGATCGGCCAGGAGATCGAACAG
CAGATTCGCGCCAAGCTGCTGGTGACTTCGGCCAATACCAAGGCCACCCCGGTAGCCGAAGATCTGGCGGACGCCGACAT
CTGA

Domains

Predicted by InterproScan.

recA bacterial DNA recombination protein (RecA)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recA Acinetobacter baylyi ADP1

75.224

96.542

0.726
  recA Acinetobacter baumannii D1279779

75.602

95.677

0.723
  recA Vibrio cholerae O1 biovar El Tor strain E7946

75.841

94.236

0.715
  recA Vibrio cholerae strain A1552

75.841

94.236

0.715
  recA Ralstonia pseudosolanacearum GMI1000

72.303

97.443

0.705
  recA Glaesserella parasuis strain SC1401

71.345

98.559

0.703
  recA Neisseria gonorrhoeae MS11

71.692

93.66

0.671
  recA Neisseria gonorrhoeae strain FA1090

71.692

93.66

0.671
  recA Neisseria gonorrhoeae MS11

71.692

93.66

0.671
  recA Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

64.832

95.335

0.618
  recA Riemerella anatipestifer ATCC 11845 = DSM 15868

61.377

97.376

0.598
  recA Helicobacter pylori 26695

60.234

98.559

0.594
  recA Helicobacter pylori strain NCTC11637

60.234

98.559

0.594
  recA Bacillus subtilis subsp. subtilis str. 168

62.813

92.219

0.579
  recA Streptococcus mutans UA159

57.864

97.118

0.562
  recA Latilactobacillus sakei subsp. sakei 23K

57.227

97.695

0.559
  recA Streptococcus pneumoniae Rx1

59.509

93.948

0.559
  recA Streptococcus pneumoniae D39

59.509

93.948

0.559
  recA Streptococcus pneumoniae R6

59.509

93.948

0.559
  recA Streptococcus pneumoniae TIGR4

59.509

93.948

0.559
  recA Streptococcus mitis SK321

56.069

99.712

0.559
  recA Streptococcus mitis NCTC 12261

58.896

93.948

0.553
  recA Streptococcus pyogenes NZ131

57.927

94.524

0.548
  recA Lactococcus lactis subsp. cremoris KW2

58.025

93.372

0.542
  recA Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

56

93.66

0.524

Multiple sequence alignment    



References

[1] B Vosman et al. (1991) Molecular cloning and functional characterization of a recA analog from Pseudomonas stutzeri and construction of a P. stutzeri recA mutant. Antonie Van Leeuwenhoek 59(2):115-23. [PMID: 1854185]