Detailed information NTG0000499

Experimentally validated

Overview

• Name: recA
• Type: Machinery gene
• Locus tag: SM12261_RS08315
• Genome accession: NZ_CP028414
• Coordinates: 1646694..1647848 (-)
• Length: 384 a.a.
• NCBI ID: WP_078228268.1
• Uniprot ID: -
• Organism: Streptococcus mitis NCTC 12261
• Function: homologous recombination Homologous recombination

Function

Upregulated in response to CSP as measured by RNA-seq.

Genomic Context

Location: 1641694..1652848

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SM12261_RS08295 (SM12261_1600)	-	1643025..1643486 (-)	462	WP_000360529.1	MarR family winged helix-turn-helix transcriptional regulator	-
SM12261_RS08300 (SM12261_1601)	-	1643700..1644345 (+)	646	Protein_1508	DUF4190 domain-containing protein	-
SM12261_RS08305 (SM12261_1602)	-	1644395..1645111 (-)	717	WP_000532882.1	YebC/PmpR family DNA-binding transcriptional regulator	-
SM12261_RS08310 (SM12261_1603)	-	1645208..1646578 (-)	1371	WP_001036250.1	MATE family efflux transporter	-
SM12261_RS08315 (SM12261_1604)	recA	1646694..1647848 (-)	1155	WP_078228268.1	recombinase RecA	Machinery gene
SM12261_RS08320 (SM12261_1605)	cinA	1647903..1649159 (-)	1257	WP_000642690.1	competence/damage-inducible protein A	Machinery gene
SM12261_RS08325 (SM12261_1606)	brpA	1649237..1650292 (-)	1056	WP_000239266.1	biofilm formation/cell division transcriptional regulator BrpA	-
SM12261_RS08330 (SM12261_1607)	-	1650300..1650818 (-)	519	WP_000455549.1	GNAT family N-acetyltransferase	-
SM12261_RS08335 (SM12261_1608)	tsaE	1650808..1651251 (-)	444	WP_000288249.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE	-
SM12261_RS08340 (SM12261_1609)	comM	1651347..1651979 (-)	633	WP_000970273.1	hypothetical protein	Regulator
SM12261_RS08345 (SM12261_1610)	ndk	1652109..1652531 (-)	423	WP_004239114.1	nucleoside-diphosphate kinase	-

Sequence

Protein

• Length: 384 a.a.
• Molecular weight: 41317.02 Da
• Isoelectric Point: 4.7523

>NTDB_id=499 SM12261_RS08315 WP_078228268.1 1646694..1647848(-) (recA) [Streptococcus mitis NCTC 12261]
MAKKPKKLDEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGERAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGP
ESSGKTTVALHAVAQAQKEGGIAAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGNPETTPGGRALKFYASVRLDVRG
STQIKGTGDQKDTNVGKETKIKVVKNKVAPPFKEAFVEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSE
NAKKYLADNPEIFDEIDHQVRVQFGLIDGEEIAESKKDEAAQVDSVNEEVTLDLGDELEIEIEE

Nucleotide

• Length: 1155 bp

>NTDB_id=499 SM12261_RS08315 WP_078228268.1 1646694..1647848(-) (recA) [Streptococcus mitis NCTC 12261]
ATGGCGAAAAAACCAAAAAAATTAGATGAAATTTCAAAAAAATTCGGGGCAGAACGTGAAAAAGCCTTGAATGACGCTCT
TAAATTGATTGAGAAAGACTTTGGTAAGGGTTCAATCATGCGTTTGGGTGAACGTGCGGAGCAAAAGGTGCAAGTGATGA
GCTCAGGTTCTTTGGCTCTTGACATTGCCCTTGGTTCAGGTGGTTATCCTAAGGGACGTATCATTGAAATCTATGGGCCA
GAGTCATCTGGTAAGACAACGGTTGCCCTTCATGCAGTTGCGCAAGCACAAAAAGAAGGTGGTATTGCTGCCTTTATCGA
TGCGGAACATGCCCTTGATCCAGCTTATGCTGCGGCCCTTGGTGTCAACATTGACGAATTGCTCTTGTCACAACCAGACT
CAGGAGAGCAAGGTCTTGAGATTGCAGGAAAATTGATTGACTCAGGAGCCGTGGATCTTGTCGTAGTCGACTCAGTTGCG
GCCCTTGTCCCTCGTGCGGAAATTGATGGAGATATCGGTGATAGCCACGTTGGTTTGCAGGCTCGTATGATGAGCCAGGC
TATGCGTAAACTTGGTGCTTCTATCAATAAAACCAAAACAATTGCCATCTTTATCAACCAATTGCGTGAAAAAGTTGGGG
TCATGTTTGGAAATCCAGAAACAACTCCTGGTGGACGTGCTCTGAAATTCTACGCATCAGTCCGTTTGGATGTTCGTGGA
AGCACACAAATCAAGGGAACTGGTGACCAAAAAGATACCAATGTCGGTAAAGAAACTAAAATCAAGGTCGTGAAAAACAA
GGTGGCTCCACCATTTAAGGAAGCCTTCGTTGAAATCATGTACGGAGAAGGTATTTCTAAGACTGGTGAACTCTTGAAAA
TCGCAAGCGATTTGGATATCATCAAAAAAGCAGGAGCTTGGTACTCTTACAAGGATGAGAAAATCGGGCAAGGTTCTGAA
AATGCTAAGAAATACTTGGCAGATAACCCAGAAATCTTTGATGAAATTGACCATCAAGTCCGTGTTCAATTTGGTTTGAT
TGATGGAGAAGAAATTGCTGAAAGCAAAAAAGATGAAGCAGCTCAAGTAGACTCTGTGAATGAAGAAGTAACCCTTGACC
TAGGCGATGAGCTTGAAATCGAAATTGAAGAATAA

Domains

Predicted by InterProScan.

• recA bacterial DNA recombination protein (RecA_N) (21-286)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
recA	Streptococcus mitis SK321	98.958	100	0.99
recA	Streptococcus pneumoniae R36A	94.072	100	0.951
recA	Streptococcus pneumoniae Rx1	94.072	100	0.951
recA	Streptococcus pneumoniae D39	94.072	100	0.951
recA	Streptococcus pneumoniae R6	94.072	100	0.951
recA	Streptococcus pneumoniae TIGR4	94.072	100	0.951
recA	Streptococcus thermophilus LMD-9	86.352	100	0.868
recA	Streptococcus pyogenes NZ131	85.079	100	0.86
recA	Streptococcus thermophilus LMG 18311	85.64	99.74	0.854
recA	Streptococcus mutans UA159	84.293	99.739	0.841
recA	Lactococcus lactis subsp. cremoris KW2	73.834	99.742	0.736
recA	Bacillus subtilis subsp. subtilis str. 168	68.788	94.828	0.652
recA	Latilactobacillus sakei subsp. sakei 23K	69.486	93.239	0.648
recA	Staphylococcus aureus strain ATCC 12600	66.066	95.965	0.634
recA	Riemerella anatipestifer ATCC 11845 = DSM 15868	62.195	95.627	0.595
recA	Neisseria gonorrhoeae strain FA1090	59.884	98.851	0.592
recA	Neisseria gonorrhoeae MS11	59.884	98.851	0.592
recA	Acinetobacter baylyi ADP1	58.192	100	0.59
recA	Acinetobacter baumannii D1279779	61.963	93.41	0.579
recA	Acinetobacter nosocomialis M2	61.656	93.143	0.574
recA	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	64.396	88.981	0.573
recA	Vibrio cholerae strain A1552	62.229	91.243	0.568
recA	Vibrio cholerae O1 biovar El Tor strain E7946	62.229	91.243	0.568
recA	Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819	58.967	95.918	0.566
recA	Glaesserella parasuis strain SC1401	61.176	91.398	0.559
recA	Ralstonia pseudosolanacearum GMI1000	59.394	93.75	0.557
recA	Pseudomonas stutzeri DSM 10701	58.896	93.948	0.553
recA	Helicobacter pylori 26695	57.751	94.813	0.548
recA	Helicobacter pylori strain NCTC11637	57.751	94.813	0.548

References

• [1]: G Salvadori et al. (2018) High-resolution profiles of the Streptococcus mitis CSP signaling pathway reveal core and strain-specific regulated genes. BMC Genomics 19(1):453. [PMID: 29898666]