Detailed information NTG0001494

Experimentally validated

Overview

• Name: recA
• Type: Machinery gene
• Locus tag: FDQ49_RS17220
• Genome accession: NZ_CP040105
• Coordinates: 3653076..3654128 (-)
• Length: 350 a.a.
• NCBI ID: WP_002049645.1
• Uniprot ID: -
• Organism: Acinetobacter nosocomialis M2
• Function: homologous recombination Homologous recombination

Function

M2 ΔrecA::aacC4 derivatives did not produce any recombinants.

Genomic Context

Location: 3648076..3659128

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FDQ49_RS17185 (FDQ49_17185)	-	3648317..3648820 (+)	504	WP_016804937.1	OmpH family outer membrane protein	-
FDQ49_RS17190 (FDQ49_17190)	lpxD	3648825..3649895 (+)	1071	WP_022575605.1	UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase	-
FDQ49_RS17195 (FDQ49_17195)	fabZ	3649902..3650387 (+)	486	WP_002049600.1	3-hydroxyacyl-ACP dehydratase FabZ	-
FDQ49_RS17200 (FDQ49_17200)	lpxA	3650384..3651172 (+)	789	WP_002049604.1	acyl-ACP--UDP-N-acetylglucosamine O-acyltransferase	-
FDQ49_RS17205 (FDQ49_17205)	-	3651227..3652111 (-)	885	WP_022575606.1	YbgF trimerization domain-containing protein	-
FDQ49_RS17215 (FDQ49_17215)	-	3652539..3653021 (-)	483	WP_004886889.1	regulatory protein RecX	-
FDQ49_RS17220 (FDQ49_17220)	recA	3653076..3654128 (-)	1053	WP_002049645.1	recombinase RecA	Machinery gene
FDQ49_RS17225 (FDQ49_17225)	-	3654274..3654714 (-)	441	WP_002049587.1	RNA-binding S4 domain-containing protein	-
FDQ49_RS17230 (FDQ49_17230)	-	3654698..3655384 (-)	687	WP_022575607.1	HAD-IA family hydrolase	-
FDQ49_RS17235 (FDQ49_17235)	-	3655745..3655981 (+)	237	WP_004886896.1	hypothetical protein	-
FDQ49_RS17240 (FDQ49_17240)	-	3656096..3656389 (+)	294	WP_004708422.1	hypothetical protein	-
FDQ49_RS17245 (FDQ49_17245)	-	3656441..3656977 (-)	537	WP_022575608.1	GNAT family N-acetyltransferase	-
FDQ49_RS17250 (FDQ49_17250)	gigD	3657021..3657470 (-)	450	WP_022575609.1	Lrp/AsnC family transcriptional regulator GigD	-
FDQ49_RS17255 (FDQ49_17255)	kynU	3657617..3658867 (+)	1251	WP_022575610.1	kynureninase	-

Sequence

Protein

• Length: 350 a.a.
• Molecular weight: 38070.43 Da
• Isoelectric Point: 4.8604

>NTDB_id=1494 FDQ49_RS17220 WP_002049645.1 3653076..3654128(-) (recA) [Acinetobacter nosocomialis M2]
MDENKSKALQAALSQIEKQFGKNTVMRLGDNTVQAVEAVSTGSLTLDIALGIGGLPKGRIIEIYGPESSGKTTMTLQAIA
QCQKSGGTCAFIDAEHALDPQYARKLGVDIDNLLVSQPDNGEQALEIADMLVRSGAIDLIVVDSVAALTPKAEIEGEMGD
SHMGLQARLMSQALRKITGNAKRSNCMVIFINQIRMKIGVMFGSPETTTGGNALKFYASVRLDIRRIGQVKEGDEIVGSE
TKVKVVKNKMAPPFREAIFQILYGKGTNQLGELVDLAVQQDIVQKAGAWYSYQGNKIGQGKNNVIRYFEENKHIAEEIER
NIREQLLTTGTNGAVQIEDDEEEPDLLLES

Nucleotide

• Length: 1053 bp

>NTDB_id=1494 FDQ49_RS17220 WP_002049645.1 3653076..3654128(-) (recA) [Acinetobacter nosocomialis M2]
ATGGATGAGAATAAAAGCAAAGCATTACAAGCCGCTTTGAGCCAAATTGAGAAACAATTTGGTAAAAATACGGTTATGCG
TCTCGGCGACAACACTGTTCAAGCAGTTGAAGCCGTATCTACAGGTTCTTTAACTTTAGACATCGCATTGGGGATTGGTG
GTTTACCAAAAGGCCGTATTATTGAAATTTATGGTCCTGAATCTTCTGGTAAAACCACAATGACATTACAAGCAATTGCT
CAATGTCAAAAGTCTGGTGGTACATGTGCCTTCATCGATGCAGAGCATGCCCTAGATCCTCAATATGCACGTAAGCTTGG
TGTAGATATTGATAACCTACTCGTTTCACAACCTGATAATGGTGAGCAAGCGCTTGAAATCGCTGATATGCTTGTTCGCT
CAGGTGCAATTGATTTAATCGTTGTGGACTCAGTAGCTGCTCTTACACCTAAAGCAGAGATTGAAGGCGAAATGGGTGAT
TCTCATATGGGTCTACAAGCACGTCTTATGAGCCAAGCACTTCGTAAAATTACTGGTAACGCTAAACGTTCAAACTGTAT
GGTTATCTTTATTAACCAAATCCGTATGAAGATTGGTGTTATGTTTGGTAGCCCAGAAACCACGACTGGTGGTAACGCTC
TTAAGTTCTATGCTTCAGTTCGTCTAGATATTCGTCGTATTGGTCAGGTAAAAGAAGGCGATGAAATTGTTGGTTCAGAA
ACTAAAGTTAAAGTCGTTAAGAATAAAATGGCTCCTCCTTTTAGAGAAGCTATTTTCCAAATTCTATACGGTAAAGGTAC
TAACCAATTAGGTGAACTTGTAGATTTGGCAGTACAGCAAGATATCGTTCAGAAAGCTGGTGCATGGTATTCATATCAAG
GCAATAAAATTGGCCAAGGTAAGAATAATGTGATCCGCTATTTTGAAGAAAATAAACATATTGCTGAAGAGATTGAACGC
AATATCCGTGAGCAATTGTTGACTACAGGTACAAATGGCGCAGTACAAATTGAAGATGATGAAGAAGAACCAGATCTTTT
ATTAGAATCTTAA

Domains

Predicted by InterProScan.

• recA bacterial DNA recombination protein (RecA_N) (7-267)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
recA	Acinetobacter baumannii D1279779	98.857	100	0.991
recA	Acinetobacter baylyi ADP1	92.507	99.427	0.92
recA	Pseudomonas stutzeri DSM 10701	73.487	100	0.735
recA	Vibrio cholerae O1 biovar El Tor strain E7946	73.394	93.429	0.686
recA	Vibrio cholerae strain A1552	73.394	93.429	0.686
recA	Ralstonia pseudosolanacearum GMI1000	70.262	97.443	0.685
recA	Glaesserella parasuis strain SC1401	71.914	92.571	0.666
recA	Neisseria gonorrhoeae strain FA1090	65.812	100	0.664
recA	Neisseria gonorrhoeae MS11	65.812	100	0.664
recA	Staphylococcus aureus strain ATCC 12600	63.61	100	0.64
recA	Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819	62.899	100	0.633
recA	Helicobacter pylori strain NCTC11637	63.158	98.559	0.622
recA	Helicobacter pylori 26695	62.865	98.559	0.62
recA	Riemerella anatipestifer ATCC 11845 = DSM 15868	63.444	96.501	0.612
recA	Bacillus subtilis subsp. subtilis str. 168	60.694	99.425	0.603
recA	Streptococcus thermophilus LMD-9	58.824	100	0.6
recA	Streptococcus pneumoniae R36A	59.827	98.857	0.591
recA	Streptococcus pneumoniae Rx1	59.827	98.857	0.591
recA	Streptococcus pneumoniae R6	59.827	98.857	0.591
recA	Streptococcus pneumoniae D39	59.827	98.857	0.591
recA	Streptococcus pneumoniae TIGR4	59.827	98.857	0.591
recA	Streptococcus thermophilus LMG 18311	58.689	100	0.589
recA	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	62.577	93.143	0.583
recA	Streptococcus mitis NCTC 12261	61.656	93.143	0.574
recA	Streptococcus mutans UA159	61.28	93.714	0.574
recA	Streptococcus mitis SK321	61.35	93.143	0.571
recA	Streptococcus pyogenes NZ131	61.538	92.857	0.571
recA	Lactococcus lactis subsp. cremoris KW2	58.514	92.286	0.54
recA	Latilactobacillus sakei subsp. sakei 23K	58.255	91.714	0.534

References

• [1]: Anne-Sophie Godeux et al. (2022) Interbacterial Transfer of Carbapenem Resistance and Large Antibiotic Resistance Islands by Natural Transformation in Pathogenic Acinetobacter. MBio 13(1):e0263121. [PMID: 35073754]