Detailed information NTG0001389

Experimentally validated

Overview

• Name: recA
• Type: Machinery gene
• Locus tag: A4U84_RS07560
• Genome accession: NZ_CP015099
• Coordinates: 1583332..1584450 (-)
• Length: 372 a.a.
• NCBI ID: WP_021112712.1
• Uniprot ID: A0A084F0G7
• Organism: Glaesserella parasuis strain SC1401
• Function: homologous recombination Homologous recombination

Function

The expression levels of candidate uptake components were significantly lower than in SC1401, respectively. These results revealed that the highly naturally competent strain SC1401 up-regulated most of the candidate natural competence-related regulons/components.

Genomic Context

Location: 1578332..1589450

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A4U84_RS07510 (A4U84_07510)	ubiK	1579667..1579927 (-)	261	WP_005713096.1	ubiquinone biosynthesis accessory factor UbiK	-
A4U84_RS07535 (A4U84_07535)	aroE	1580612..1581430 (-)	819	WP_005713094.1	shikimate dehydrogenase	-
A4U84_RS07540 (A4U84_07540)	-	1581434..1581988 (-)	555	WP_005713092.1	Sua5/YciO/YrdC/YwlC family protein	-
A4U84_RS07545 (A4U84_07545)	-	1581988..1582518 (-)	531	WP_021112716.1	DNA topoisomerase family protein	-
A4U84_RS07550 (A4U84_07550)	yqfB	1582526..1582831 (-)	306	WP_005713088.1	N(4)-acetylcytidine aminohydrolase	-
A4U84_RS07555 (A4U84_07555)	recX	1582824..1583279 (-)	456	WP_005713086.1	recombination regulator RecX	-
A4U84_RS07560 (A4U84_07560)	recA	1583332..1584450 (-)	1119	WP_021112712.1	recombinase RecA	Machinery gene
A4U84_RS07565 (A4U84_07565)	-	1584583..1585188 (-)	606	WP_021114078.1	aminotransferase class IV family protein	-
A4U84_RS07570 (A4U84_07570)	-	1585166..1586137 (-)	972	WP_021116195.1	aminodeoxychorismate synthase component I	-
A4U84_RS11150	-	1586170..1586364 (+)	195	Protein_1475	cation transporter	-
A4U84_RS07575 (A4U84_07575)	tehB	1586587..1587450 (+)	864	WP_062924243.1	SAM-dependent methyltransferase TehB	-
A4U84_RS07580 (A4U84_07580)	dusA	1587565..1588536 (+)	972	WP_062924244.1	tRNA dihydrouridine(20/20a) synthase DusA	-
A4U84_RS12265	-	1589128..1589340 (+)	213	WP_016527504.1	hypothetical protein	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 40277.89 Da
• Isoelectric Point: 4.7724

>NTDB_id=1389 A4U84_RS07560 WP_021112712.1 1583332..1584450(-) (recA) [Glaesserella parasuis strain SC1401]
MAENKKSSKNNTVVKQVEPEQKKQALEAALAQIEKQFGKGSIMKLGQTEQLDIESVSTGSLGLDLALGIGGLPMGRIVEI
YGPESSGKTTLTLSLIAQAQKGNKTCAFIDAEHALDPVYARNLGVNTDELLISQPDNGEQALEICDALVRSGAVDVIIVD
SVAALTPKAEIEGDMGDSHMGLQARLMSQALRKLTANIKNTNCLVVFINQIRMKIGVMFGNPETTTGGNALKFYASVRLD
IRRCGVVKDGDEIIGSETKVKVVKNKVAPPFREVQFDIMYGEGISRMNELLILAEANGFIKKSGAWFSYDGTKIGQGKNN
AVKWLKENPEIADKLEQEIRNTLASNPNSVLKATASDSNDESDVEEFSEEEL

Nucleotide

• Length: 1119 bp

>NTDB_id=1389 A4U84_RS07560 WP_021112712.1 1583332..1584450(-) (recA) [Glaesserella parasuis strain SC1401]
ATGGCTGAGAATAAGAAATCTTCAAAAAACAATACCGTCGTTAAACAAGTTGAACCAGAACAGAAAAAACAAGCATTGGA
AGCGGCACTTGCTCAAATTGAAAAACAATTTGGTAAAGGCTCTATTATGAAATTGGGGCAGACTGAACAATTAGATATTG
AGTCTGTTTCAACGGGATCATTAGGACTAGACCTTGCTCTTGGTATTGGTGGCTTGCCAATGGGACGTATTGTTGAAATC
TATGGGCCAGAATCATCAGGTAAAACAACCTTAACACTTTCTTTAATTGCCCAAGCTCAAAAAGGCAATAAAACCTGTGC
ATTTATTGATGCGGAACACGCATTAGATCCTGTCTATGCTCGCAATTTAGGGGTAAATACCGATGAATTACTAATTTCTC
AGCCAGATAACGGCGAACAAGCTCTTGAAATCTGTGATGCGTTAGTGCGTTCAGGGGCAGTTGATGTCATTATTGTGGAC
TCTGTCGCTGCACTCACGCCAAAAGCTGAAATTGAAGGGGATATGGGTGACTCGCATATGGGCTTACAAGCACGTTTAAT
GTCACAAGCGTTGCGTAAACTTACTGCGAACATCAAAAATACCAACTGTTTAGTTGTATTTATCAACCAAATCCGTATGA
AAATTGGTGTAATGTTTGGTAATCCAGAAACCACCACAGGCGGTAATGCACTGAAATTCTATGCCTCTGTTCGTTTAGAT
ATTCGTCGTTGTGGTGTAGTCAAAGATGGCGATGAAATTATTGGTAGTGAAACAAAAGTAAAAGTAGTAAAAAACAAAGT
TGCACCGCCATTCCGTGAAGTACAATTTGATATTATGTACGGCGAAGGGATTTCTCGTATGAATGAGCTACTGATTTTAG
CTGAAGCAAATGGCTTTATTAAGAAATCAGGTGCTTGGTTCTCTTACGACGGAACGAAAATTGGACAAGGTAAAAATAAT
GCAGTGAAATGGTTAAAAGAGAACCCTGAAATTGCAGATAAACTTGAACAAGAGATCCGAAATACATTGGCGTCAAACCC
AAATAGCGTATTAAAAGCGACGGCAAGCGACAGCAATGATGAAAGCGATGTCGAAGAGTTTAGCGAAGAAGAACTATAA

Domains

Predicted by InterproScan.

• recA bacterial DNA recombination protein (RecA) (24-285)

3D structure

• AlphaFold DB: A0A084F0G7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
recA	Pseudomonas stutzeri DSM 10701	71.345	98.559	0.703
recA	Vibrio cholerae O1 biovar El Tor strain E7946	69.516	99.153	0.689
recA	Vibrio cholerae strain A1552	69.516	99.153	0.689
recA	Neisseria gonorrhoeae MS11	67.521	100	0.681
recA	Neisseria gonorrhoeae MS11	67.521	100	0.681
recA	Neisseria gonorrhoeae strain FA1090	67.521	100	0.681
recA	Acinetobacter baylyi ADP1	69.298	97.994	0.679
recA	Acinetobacter baumannii D1279779	71.605	92.837	0.665
recA	Ralstonia pseudosolanacearum GMI1000	70.031	92.898	0.651
recA	Riemerella anatipestifer ATCC 11845 = DSM 15868	63.037	100	0.641
recA	Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819	65.031	95.044	0.618
recA	Bacillus subtilis subsp. subtilis str. 168	60.58	99.138	0.601
recA	Streptococcus pneumoniae Rx1	57.641	100	0.578
recA	Streptococcus pneumoniae D39	57.641	100	0.578
recA	Streptococcus pneumoniae TIGR4	57.641	100	0.578
recA	Streptococcus pneumoniae R6	57.641	100	0.578
recA	Helicobacter pylori 26695	60.976	94.524	0.576
recA	Helicobacter pylori strain NCTC11637	60.671	94.524	0.573
recA	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	64.396	88.981	0.573
recA	Streptococcus mitis NCTC 12261	61.176	91.398	0.559
recA	Streptococcus mitis SK321	60.588	91.398	0.554
recA	Streptococcus mutans UA159	60.294	91.398	0.551
recA	Streptococcus pyogenes NZ131	59.412	91.398	0.543
recA	Latilactobacillus sakei subsp. sakei 23K	59.19	90.423	0.535
recA	Lactococcus lactis subsp. cremoris KW2	57.558	92.473	0.532

References

• [1]: Ke Dai et al. (2018) Basic Characterization of Natural Transformation in a Highly Transformable Haemophilus parasuis Strain SC1401. Frontiers in Cellular And Infection Microbiology 8:32. [PMID: 29473023]