Detailed information NTG0001130

Experimentally validated

Overview

• Name: recA
• Type: Machinery gene
• Locus tag: NGFG_RS03960
• Genome accession: NC_022240
• Coordinates: 739455..740501 (+)
• Length: 348 a.a.
• NCBI ID: WP_003688695.1
• Uniprot ID: Q5F8M9
• Organism: Neisseria gonorrhoeae MS11
• Function: homologous recombination Homologous recombination

Function

RecA is necessary to maintain newly acquired chromosomal markers via homologous recombination into the genome, and RecA-deficient gonococci are not transformable.

Genomic Context

Location: 734455..745501

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NGFG_RS15480	-	735021..735230 (-)	210	WP_003688699.1	hypothetical protein	-
NGFG_RS03945 (NGFG_01048)	dinB	735329..736387 (+)	1059	WP_003695315.1	DNA polymerase IV	-
NGFG_RS03950 (NGFG_01047)	rep	736457..738471 (-)	2015	Protein_752	DNA helicase Rep	-
NGFG_RS03955 (NGFG_01046)	aroD	738491..739255 (-)	765	WP_003688696.1	type I 3-dehydroquinate dehydratase	-
NGFG_RS03960 (NGFG_01045)	recA	739455..740501 (+)	1047	WP_003688695.1	recombinase RecA	Machinery gene
NGFG_RS03970 (NGFG_01044)	-	741154..741489 (-)	336	WP_003688693.1	YbaB/EbfC family nucleoid-associated protein	-
NGFG_RS03975 (NGFG_01043)	dnaX	741570..743693 (-)	2124	WP_003695312.1	DNA polymerase III subunit gamma/tau	-

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
recX	recA	negative effect

Sequence

Protein

• Length: 348 a.a.
• Molecular weight: 37642.87 Da
• Isoelectric Point: 4.8330

>NTDB_id=1130 NGFG_RS03960 WP_003688695.1 739455..740501(+) (recA) [Neisseria gonorrhoeae MS11]
MSDDKSKALAAALAQIEKSFGKGAIMKMDGSQQEENLEVISTGSLGLDLALGVGGLPRGRIVEIFGPESSGKTTLCLEAV
AQCQKNGGVCAFVDAEHAFDPVYARKLGVKVEELYLSQPDTGEQALEICDTLVRSGGIDMVVVDSVAALVPKAEIEGDMG
DSHVGLQARLMSQALRKLTGHIKKTNTLVVFINQIRMKIGVMFGSPETTTGGNALKFYSSVRLDIRRTGSIKKGEEVLGN
ETRVKVIKNKVAPPFRQAEFDILYGEGISWEGELIDIGVKNDIINKSGAWYSYNGAKIGQGKDNVRVWLKENPEISDEID
AKIRALNGVEMHITEGTQDETDGERPEE

Nucleotide

• Length: 1047 bp

>NTDB_id=1130 NGFG_RS03960 WP_003688695.1 739455..740501(+) (recA) [Neisseria gonorrhoeae MS11]
ATGTCAGACGACAAAAGCAAAGCCCTTGCCGCCGCACTGGCGCAAATCGAAAAAAGTTTCGGCAAAGGCGCCATCATGAA
AATGGACGGCAGCCAGCAGGAAGAAAACCTCGAAGTCATTTCCACCGGATCGCTCGGATTAGACCTCGCCCTCGGAGTCG
GCGGCCTGCCGCGCGGGCGCATCGTCGAAATCTTCGGCCCCGAATCCTCCGGCAAAACCACACTCTGCCTCGAAGCCGTC
GCCCAATGCCAGAAAAACGGCGGCGTGTGCGCCTTTGTCGATGCCGAACACGCCTTCGATCCCGTTTACGCCCGCAAACT
CGGCGTAAAAGTCGAAGAGCTTTACCTGTCCCAGCCCGATACCGGCGAACAGGCTTTGGAAATCTGCGACACGCTCGTCC
GTTCGGGCGGCATAGATATGGTAGTCGTCGATTCCGTAGCCGCACTCGTCCCCAAAGCCGAAATCGAAGGCGATATGGGG
GACAGCCACGTCGGACTGCAGGCGCGCCTGATGAGTCAGGCTTTGCGCAAACTGACCGGACACATCAAAAAAACCAATAC
GCTGGTTGTGTTCATCAACCAAATCCGAATGAAGATCGGCGTAATGTTCGGCAGCCCCGAGACCACGACCGGCGGCAACG
CGCTGAAATTCTATTCTTCCGTCCGCCTCGACATCCGCCGCACCGGATCCATCAAAAAAGGCGAAGAAGTATTGGGCAAC
GAAACCCGCGTCAAAGTCATCAAAAACAAAGTCGCCCCCCCGTTCCGTCAGGCAGAGTTTGACATCCTCTACGGAGAAGG
CATCAGTTGGGAAGGCGAATTGATCGACATCGGCGTGAAAAACGACATCATCAACAAATCCGGCGCGTGGTACAGCTACA
ACGGCGCGAAAATCGGTCAGGGCAAAGACAACGTCCGCGTCTGGCTGAAAGAAAACCCCGAAATTTCCGACGAAATCGAC
GCAAAAATCCGCGCCCTCAACGGCGTAGAAATGCACATCACCGAAGGGACGCAGGACGAAACCGACGGCGAACGACCCGA
AGAATAA

Domains

Predicted by InterProScan.

• recA bacterial DNA recombination protein (RecA_N) (7-269)

3D structure

• AlphaFold DB: Q5F8M9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
recA	Neisseria gonorrhoeae strain FA1090	100	100	1
recA	Ralstonia pseudosolanacearum GMI1000	74.468	94.54	0.704
recA	Vibrio cholerae strain A1552	66.949	100	0.681
recA	Vibrio cholerae O1 biovar El Tor strain E7946	66.949	100	0.681
recA	Glaesserella parasuis strain SC1401	67.521	100	0.681
recA	Pseudomonas stutzeri DSM 10701	71.692	93.66	0.671
recA	Acinetobacter baylyi ADP1	66.859	99.713	0.667
recA	Acinetobacter nosocomialis M2	65.812	100	0.664
recA	Acinetobacter baumannii D1279779	65.517	100	0.655
recA	Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819	65.103	99.417	0.647
recA	Riemerella anatipestifer ATCC 11845 = DSM 15868	67.073	95.627	0.641
recA	Staphylococcus aureus strain ATCC 12600	65.192	97.695	0.637
recA	Bacillus subtilis subsp. subtilis str. 168	63.584	99.425	0.632
recA	Helicobacter pylori 26695	67.077	93.66	0.628
recA	Helicobacter pylori strain NCTC11637	66.769	93.66	0.625
recA	Streptococcus mutans UA159	60.684	100	0.612
recA	Streptococcus pneumoniae R36A	59.544	100	0.601
recA	Streptococcus pneumoniae Rx1	59.544	100	0.601
recA	Streptococcus pneumoniae D39	59.544	100	0.601
recA	Streptococcus pneumoniae R6	59.544	100	0.601
recA	Streptococcus pneumoniae TIGR4	59.544	100	0.601
recA	Streptococcus thermophilus LMD-9	59.827	99.425	0.595
recA	Streptococcus thermophilus LMG 18311	59.827	99.425	0.595
recA	Streptococcus mitis NCTC 12261	59.884	98.851	0.592
recA	Latilactobacillus sakei subsp. sakei 23K	62.691	93.966	0.589
recA	Streptococcus pyogenes NZ131	61.963	93.678	0.58
recA	Streptococcus mitis SK321	60.606	94.828	0.575
recA	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	60.121	95.115	0.572
recA	Lactococcus lactis subsp. cremoris KW2	60.303	94.828	0.572

References

• [1]: Paul M Duffin et al. (2016) DprA is required for natural transformation and affects pilin variation in Neisseria gonorrhoeae. Microbiology (Reading, England) 162(9):1620-1628. [PMID: 27469477]
• [2]: Elizabeth A Stohl et al. (2011) Purification and characterization of the RecA protein from Neisseria gonorrhoeae. PloS One 6(2):e17101. [PMID: 21359151]
• [3]: J M Koomey et al. (1987) Cloning of the recA gene of Neisseria gonorrhoeae and construction of gonococcal recA mutants. Journal of Bacteriology 169(2):790-5. [PMID: 3100504]