Detailed information NTG0001211

Experimentally validated

Overview

• Name: recA
• Type: Machinery gene
• Locus tag: C694_RS00795
• Genome accession: NC_018939
• Coordinates: 162930..163973 (+)
• Length: 347 a.a.
• NCBI ID: WP_000963128.1
• Uniprot ID: A0AAC8N0B3
• Organism: Helicobacter pylori 26695
• Function: homologous recombination Homologous recombination

Function

Compared to the wild-type strains, isogenic H. pylori recA deletion mutants displayed increased sensitivity to ultraviolet light and abolished general homologous recombination.

Genomic Context

Location: 157930..168973

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C694_RS00760 (C694_00725)	-	158524..158742 (+)	219	WP_001862885.1	cytochrome c oxidase, cbb3-type, CcoQ subunit	-
C694_RS00765 (C694_00730)	ccoP	158744..159621 (+)	878	Protein_139	cytochrome-c oxidase, cbb3-type subunit III	-
C694_RS00770 (C694_00735)	-	159632..159838 (+)	207	WP_000670506.1	DUF4006 family protein	-
C694_RS00775 (C694_00745)	-	159939..160523 (+)	585	WP_001221992.1	hypothetical protein	-
C694_RS00780 (C694_00750)	-	160536..161126 (+)	591	WP_000660370.1	hypothetical protein	-
C694_RS00785 (C694_00755)	-	161204..161971 (+)	768	WP_000506345.1	hypothetical protein	-
C694_RS00790 (C694_00760)	-	161968..162831 (-)	864	WP_000626212.1	menaquinone biosynthesis family protein	-
C694_RS00795 (C694_00765)	recA	162930..163973 (+)	1044	WP_000963128.1	recombinase RecA	Machinery gene
C694_RS00800 (C694_00770)	eno	163985..165265 (+)	1281	WP_000955673.1	phosphopyruvate hydratase	-
C694_RS00805 (C694_00775)	-	165258..165533 (+)	276	WP_000146222.1	hypothetical protein	-
C694_RS00810 (C694_00780)	-	165551..166147 (+)	597	WP_001862880.1	AMIN domain-containing protein	-
C694_RS00815 (C694_00785)	-	166152..166640 (+)	489	WP_001164290.1	shikimate kinase	-
C694_RS00820 (C694_00790)	-	166662..167618 (+)	957	WP_000952264.1	PDC sensor domain-containing protein	-
C694_RS00825 (C694_00795)	rfaJ	167615..168733 (-)	1119	WP_000022419.1	HP0159 family lipopolysaccharide 1,6-glucosyltransferase	-

Sequence

Protein

• Length: 347 a.a.
• Molecular weight: 37686.39 Da
• Isoelectric Point: 5.5047

>NTDB_id=1211 C694_RS00795 WP_000963128.1 162930..163973(+) (recA) [Helicobacter pylori 26695]
MAIDEDKQKAISLAIKQIDKVFGKGALVRLGDKQVEKIDSISTGSLGLDLALGIGGVPKGRIIEIYGPESSGKTTLSLHI
IAECQKNGGVCAFIDAEHALDVHYAKRLGVDTENLLVSQPDTGEQALEILETITRSGGIDLVVVDSVAALTPKAEIDGDM
GDQHVGLQARLMSHALRKITGVLHKMNTTLIFINQIRMKIGMMGYGSPETTTGGNALKFYASVRIDIRRIASLKQNEQHI
GNRAKAKVVKNKVAPPFREAEFDIMFGEGISKEGEIIDYGVKLDIVDKSGAWLSYQDKKLGQGRENAKALLKEDKALADE
ITLKIKESIGSNEEIMPLPDEPLEEME

Nucleotide

• Length: 1044 bp

>NTDB_id=1211 C694_RS00795 WP_000963128.1 162930..163973(+) (recA) [Helicobacter pylori 26695]
ATGGCAATAGATGAAGACAAACAAAAAGCGATTTCTTTAGCGATCAAACAAATTGATAAGGTTTTTGGTAAGGGGGCGTT
GGTGCGCCTTGGGGATAAGCAAGTAGAAAAGATTGACTCTATTTCTACAGGCTCGTTAGGGTTGGATCTGGCTTTAGGGA
TTGGGGGCGTTCCAAAGGGTAGGATCATTGAAATTTATGGGCCAGAGTCAAGTGGGAAGACCACTCTAAGCTTGCATATT
ATTGCAGAATGCCAAAAAAATGGCGGCGTGTGCGCGTTCATTGACGCTGAGCATGCCCTAGATGTGCATTACGCTAAGAG
ATTGGGCGTGGATACGGAAAATCTACTCGTTTCCCAACCTGATACAGGCGAGCAAGCTTTAGAGATTTTAGAAACGATCA
CCAGAAGCGGAGGGATTGATTTAGTGGTGGTGGATTCTGTGGCGGCTCTTACGCCTAAAGCGGAGATTGATGGGGATATG
GGCGATCAGCATGTGGGCTTGCAAGCAAGGCTTATGAGCCATGCGTTAAGAAAAATCACCGGTGTCTTGCACAAGATGAA
CACTACTCTCATTTTTATCAATCAAATCAGGATGAAGATTGGCATGATGGGTTATGGGAGTCCAGAGACCACAACCGGAG
GTAACGCCTTAAAATTCTATGCGAGCGTTAGGATTGATATTAGAAGGATTGCCTCCCTCAAACAAAACGAACAGCATATC
GGTAATAGGGCTAAAGCCAAAGTGGTTAAAAATAAAGTCGCTCCGCCTTTTAGAGAAGCGGAATTTGACATCATGTTTGG
GGAAGGGATTTCTAAAGAGGGCGAAATCATTGATTACGGTGTGAAATTAGACATTGTGGATAAGAGTGGGGCATGGCTTA
GCTACCAGGATAAAAAGCTAGGGCAAGGCAGAGAAAACGCTAAAGCCTTACTGAAAGAAGACAAAGCCCTAGCGGATGAA
ATCACTCTTAAGATTAAAGAGAGTATTGGCTCTAATGAAGAGATCATGCCCTTACCGGATGAGCCTTTAGAAGAAATGGA
ATAA

Domains

Predicted by InterproScan.

• recA bacterial DNA recombination protein (RecA) (9-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
recA	Helicobacter pylori strain NCTC11637	99.424	100	0.994
recA	Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819	79.268	95.627	0.758
recA	Neisseria gonorrhoeae MS11	67.077	93.66	0.628
recA	Neisseria gonorrhoeae MS11	67.077	93.66	0.628
recA	Neisseria gonorrhoeae strain FA1090	67.077	93.66	0.628
recA	Riemerella anatipestifer ATCC 11845 = DSM 15868	64.458	96.793	0.624
recA	Bacillus subtilis subsp. subtilis str. 168	63.081	98.851	0.624
recA	Acinetobacter baumannii D1279779	63.914	94.236	0.602
recA	Ralstonia pseudosolanacearum GMI1000	62.202	96.83	0.602
recA	Acinetobacter baylyi ADP1	63.609	94.236	0.599
recA	Pseudomonas stutzeri DSM 10701	60.234	98.559	0.594
recA	Vibrio cholerae O1 biovar El Tor strain E7946	61.012	96.83	0.591
recA	Vibrio cholerae strain A1552	61.012	96.83	0.591
recA	Glaesserella parasuis strain SC1401	60.976	94.524	0.576
recA	Streptococcus pneumoniae Rx1	56.125	100	0.568
recA	Streptococcus pneumoniae D39	56.125	100	0.568
recA	Streptococcus pneumoniae R6	56.125	100	0.568
recA	Streptococcus pneumoniae TIGR4	56.125	100	0.568
recA	Latilactobacillus sakei subsp. sakei 23K	54.902	100	0.565
recA	Lactococcus lactis subsp. cremoris KW2	58.055	94.813	0.55
recA	Streptococcus pyogenes NZ131	57.879	95.101	0.55
recA	Streptococcus mitis NCTC 12261	57.751	94.813	0.548
recA	Streptococcus mitis SK321	57.447	94.813	0.545
recA	Streptococcus mutans UA159	56.287	96.254	0.542
recA	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	56.442	93.948	0.53

References

• [1]: Prashant P Damke et al. (2019) Identification of the periplasmic DNA receptor for natural transformation of Helicobacter pylori. Nature Communications 10(1):5357. [PMID: 31767852]
• [2]: Emilie Orillard et al. (2011) Biochemical and cellular characterization of Helicobacter pylori RecA, a protein with high-level constitutive expression. Journal of Bacteriology 193(23):6490-7. [PMID: 21949074]
• [3]: W Schmitt et al. (1995) Cloning of the Helicobacter pylori recA gene and functional characterization of its product. Molecular & General Genetics : MGG 248(5):563-72. [PMID: 7476856]