Detailed information    

experimental Experimentally validated

Overview

Name   recA   Type   Machinery gene
Locus tag   ABD1_RS10195 Genome accession   NC_020547
Coordinates   2137334..2138383 (+) Length   349 a.a.
NCBI ID   WP_000344167.1    Uniprot ID   A0A009SQ18
Organism   Acinetobacter baumannii D1279779     
Function   homologous recombination   
Homologous recombination

Function

We examined the 33 knockout mutants by comparing their transformation efficiency with wild-type W068. No knockout strains were affected in growth, but 28 of these mutants were severely or completely impaired in natural transformability. These mutations were in 18 genes related to T4P (pilF, pilQ, tsaP, pilM, pilN, pilO, pilP, pilB, pilC, pilT, pilD, pilE, pilY2, pilY1, pilX, pilW, pilV, and fimU), six genes related to DNA uptake and processing (comEA, comA, comF, priA, dprA, and recA), two genes related to T2SS (xcpV and xcpW), and the crp and tonB2 genes.


Genomic Context

Location: 2132334..2143383
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABD1_RS10160 (ABD1_19810) gigD 2133721..2134170 (+) 450 WP_001099948.1 Lrp/AsnC family transcriptional regulator GigD -
  ABD1_RS10165 (ABD1_19820) - 2134216..2134752 (+) 537 WP_000573219.1 GNAT family N-acetyltransferase -
  ABD1_RS10170 (ABD1_19830) - 2134804..2135097 (-) 294 WP_000689661.1 hypothetical protein -
  ABD1_RS10175 (ABD1_19840) - 2135212..2135448 (-) 237 WP_000525041.1 hypothetical protein -
  ABD1_RS10180 (ABD1_19850) - 2135547..2135906 (-) 360 WP_000798357.1 YcgJ family protein -
  ABD1_RS10185 (ABD1_19860) - 2136078..2136764 (+) 687 WP_001045480.1 HAD-IA family hydrolase -
  ABD1_RS10190 (ABD1_19870) - 2136748..2137188 (+) 441 WP_000040959.1 RNA-binding S4 domain-containing protein -
  ABD1_RS10195 (ABD1_19880) recA 2137334..2138383 (+) 1050 WP_000344167.1 recombinase RecA Machinery gene
  ABD1_RS10200 (ABD1_19890) - 2138437..2138919 (+) 483 WP_000476756.1 regulatory protein RecX -
  ABD1_RS10205 (ABD1_19900) - 2139348..2140232 (+) 885 WP_001178068.1 YbgF trimerization domain-containing protein -
  ABD1_RS10210 (ABD1_19910) lpxA 2140287..2141075 (-) 789 WP_000064877.1 acyl-ACP--UDP-N-acetylglucosamine O-acyltransferase -
  ABD1_RS10215 (ABD1_19920) fabZ 2141072..2141557 (-) 486 WP_000140523.1 3-hydroxyacyl-ACP dehydratase FabZ -
  ABD1_RS10220 (ABD1_19930) lpxD 2141564..2142634 (-) 1071 WP_000865435.1 UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase -
  ABD1_RS10225 (ABD1_19940) - 2142639..2143142 (-) 504 WP_001037943.1 OmpH family outer membrane protein -

Sequence

Protein


Download         Length: 349 a.a.        Molecular weight: 37904.25 Da        Isoelectric Point: 4.7769

>NTDB_id=1074 ABD1_RS10195 WP_000344167.1 2137334..2138383(+) (recA) [Acinetobacter baumannii D1279779]
MDENKSKALQAALSQIEKQFGKNTVMRLGDNTVQAVEAVSTGSLTLDIALGIGGLPKGRIIEIYGPESSGKTTMTLQAIA
QCQKSGGTCAFIDAEHALDPQYARKLGVDIDNLLVSQPDNGEQALEIADMLVRSGAIDLIVVDSVAALTPKAEIEGEMGD
SHMGLQARLMSQALRKITGNAKRSNCMVIFINQIRMKIGVMFGSPETTTGGNALKFYASVRLDIRRIGQVKEGDEIVGSE
TKVKVVKNKMAPPFKEAIFQILYGKGTNQLGELVDLAVQQDIVQKAGAWYSYQGNKIGQGKNNVIRYFEENNQIAEEIER
NIREQLLTTGTNGAVQIEDEEEPDLLLES

Nucleotide


Download         Length: 1050 bp        

>NTDB_id=1074 ABD1_RS10195 WP_000344167.1 2137334..2138383(+) (recA) [Acinetobacter baumannii D1279779]
ATGGATGAGAATAAAAGCAAAGCATTACAAGCCGCTTTGAGCCAAATTGAGAAGCAATTTGGTAAAAATACGGTTATGCG
TCTTGGTGATAACACTGTTCAAGCAGTTGAAGCCGTATCTACAGGTTCTTTAACTTTAGACATCGCATTGGGGATTGGGG
GTTTACCAAAAGGCCGTATTATCGAAATTTATGGTCCTGAATCTTCTGGTAAAACCACAATGACATTGCAAGCAATTGCT
CAATGTCAAAAATCTGGTGGTACATGTGCCTTCATTGATGCTGAGCACGCCCTAGACCCTCAATATGCACGCAAACTTGG
TGTAGATATTGATAACCTACTTGTTTCACAACCCGACAATGGTGAGCAAGCACTTGAAATTGCTGACATGCTTGTCCGTT
CAGGCGCAATTGATTTAATCGTTGTGGACTCGGTAGCTGCACTTACACCTAAAGCAGAAATCGAAGGTGAGATGGGTGAC
TCTCATATGGGTCTACAAGCGCGTCTTATGAGCCAGGCACTTCGTAAAATTACGGGTAATGCTAAACGTTCAAACTGTAT
GGTTATCTTCATTAACCAGATTCGTATGAAAATTGGTGTTATGTTTGGTAGCCCAGAAACTACCACTGGTGGTAACGCTC
TTAAATTCTACGCTTCAGTTCGTTTAGATATTCGTCGTATCGGTCAAGTAAAAGAAGGCGATGAAATTGTTGGTTCAGAA
ACTAAGGTTAAAGTCGTTAAGAACAAAATGGCTCCTCCGTTTAAAGAAGCTATTTTCCAAATTCTATACGGTAAAGGTAC
TAACCAACTAGGTGAACTTGTAGATTTAGCTGTACAACAGGATATCGTACAGAAAGCTGGTGCATGGTATTCATATCAAG
GCAATAAAATTGGTCAAGGTAAAAACAATGTAATTCGCTATTTTGAAGAAAATAATCAAATAGCTGAAGAAATTGAACGT
AATATCCGTGAACAATTGCTGACTACAGGCACAAATGGTGCAGTTCAAATTGAAGATGAAGAAGAGCCAGATCTTTTATT
AGAATCGTAA

Domains

Predicted by InterproScan.

recA bacterial DNA recombination protein (RecA)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A009SQ18

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recA Acinetobacter baylyi ADP1

93.084

99.427

0.926
  recA Pseudomonas stutzeri DSM 10701

75.602

95.677

0.723
  recA Vibrio cholerae strain A1552

73.7

93.696

0.691
  recA Vibrio cholerae O1 biovar El Tor strain E7946

73.7

93.696

0.691
  recA Ralstonia pseudosolanacearum GMI1000

70.058

97.727

0.685
  recA Glaesserella parasuis strain SC1401

71.605

92.837

0.665
  recA Neisseria gonorrhoeae strain FA1090

65.517

100

0.655
  recA Neisseria gonorrhoeae MS11

65.517

100

0.655
  recA Neisseria gonorrhoeae MS11

65.517

100

0.655
  recA Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

61.808

100

0.618
  recA Riemerella anatipestifer ATCC 11845 = DSM 15868

63.746

96.501

0.615
  recA Helicobacter pylori strain NCTC11637

64.22

94.236

0.605
  recA Helicobacter pylori 26695

63.914

94.236

0.602
  recA Streptococcus pyogenes NZ131

58.924

100

0.596
  recA Bacillus subtilis subsp. subtilis str. 168

61.243

97.126

0.595
  recA Streptococcus mitis SK321

60.411

97.708

0.59
  recA Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

62.883

93.41

0.587
  recA Streptococcus pneumoniae Rx1

61.963

93.41

0.579
  recA Streptococcus mitis NCTC 12261

61.963

93.41

0.579
  recA Streptococcus pneumoniae D39

61.963

93.41

0.579
  recA Streptococcus pneumoniae R6

61.963

93.41

0.579
  recA Streptococcus pneumoniae TIGR4

61.963

93.41

0.579
  recA Streptococcus mutans UA159

61.585

93.983

0.579
  recA Lactococcus lactis subsp. cremoris KW2

58.824

92.55

0.544
  recA Latilactobacillus sakei subsp. sakei 23K

58.567

91.977

0.539

Multiple sequence alignment    



References

[1] Yuan Hu et al. (2021) Natural Transformation in Acinetobacter baumannii W068: A Genetic Analysis Reveals the Involvements of the CRP, XcpV, XcpW, TsaP, and TonB2. Frontiers in Microbiology 12:738034. [PMID: 35126321]