Detailed information NTG0000408

Experimentally validated

Overview

• Name: recA
• Type: Machinery gene
• Locus tag: SMU_RS09530
• Genome accession: NC_004350
• Coordinates: 1956459..1957610 (-)
• Length: 383 a.a.
• NCBI ID: WP_002262392.1
• Uniprot ID: P27624
• Organism: Streptococcus mutans UA159
• Function: homologous recombination Homologous recombination

Function

Deletion of the genes for recombinase A, recA, DNA processing protein, dprA and single-stranded DNA-binding protein, ssbA, produced results comparable with those from other bacteria, supporting the contention that these proteins have similar functions in S. mutans competence.

Genomic Context

Location: 1951459..1962610

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SMU_RS09490 (SMU.2077c)	-	1952844..1953143 (-)	300	WP_002262384.1	DUF1292 domain-containing protein	-
SMU_RS09495 (SMU.2078c)	ruvX	1953224..1953643 (-)	420	WP_002262385.1	Holliday junction resolvase RuvX	-
SMU_RS09500 (SMU.2079c)	-	1953640..1953909 (-)	270	WP_002262386.1	IreB family regulatory phosphoprotein	-
SMU_RS09505 (SMU.2080)	brsR	1954077..1954511 (+)	435	WP_002262387.1	bacteriocin genes transcriptional regulator BrsR	Regulator
SMU_RS09510 (SMU.2081)	brsM	1954524..1954970 (+)	447	WP_002262388.1	bacteriocin genes regulator BrsM	Regulator
SMU_RS09515	-	1954961..1955173 (-)	213	WP_002262389.1	hypothetical protein	-
SMU_RS09520 (SMU.2083c)	-	1955300..1955725 (-)	426	WP_002262390.1	hypothetical protein	-
SMU_RS09525 (SMU.2084c)	spx	1955976..1956374 (-)	399	WP_002262391.1	transcriptional regulator Spx	-
SMU_RS09530 (SMU.2085)	recA	1956459..1957610 (-)	1152	WP_002262392.1	recombinase RecA	Machinery gene
SMU_RS09535 (SMU.2086)	cinA	1957650..1958906 (-)	1257	WP_002262393.1	competence/damage-inducible protein A	Machinery gene
SMU_RS09540 (SMU.2087)	-	1959055..1959615 (-)	561	WP_002262394.1	DNA-3-methyladenine glycosylase I	-
SMU_RS09545 (SMU.2088)	ruvA	1959697..1960290 (-)	594	WP_002262395.1	Holliday junction branch migration protein RuvA	-
SMU_RS09550 (SMU.2089)	mutL	1960473..1962428 (-)	1956	WP_002262396.1	DNA mismatch repair endonuclease MutL	-

Sequence

Protein

• Length: 383 a.a.
• Molecular weight: 41420.01 Da
• Isoelectric Point: 4.9881

>NTDB_id=408 SMU_RS09530 WP_002262392.1 1956459..1957610(-) (recA) [Streptococcus mutans UA159]
MAKRIKKTEEITKKFGDERKKALDDALKNIEKDFGKGAVMRLGERAEQKVQVMSSGSLALDIALGAGGYPKGRIVEIYGP
ESSGKTTVALHAVAQAQKDGGIAAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLSASINKTKTIAIFINQLREKVGIMFGNPETTPGGRALKFYSSVRLDVRG
NTQIKGTGEQKDSNIGKETKIKVVKNKVAPPFKEAFVEIIYGEGISRTGELVKIASDLGIIQKAGAWYSYNGEKIGQGSE
NAKKFLADNPEIFDDIDHKVRVQYGLIEEDNTEEKQSSKEKETDEKADKNLVLELDDTIELED

Nucleotide

• Length: 1152 bp

>NTDB_id=408 SMU_RS09530 WP_002262392.1 1956459..1957610(-) (recA) [Streptococcus mutans UA159]
TTGGCCAAAAGAATAAAAAAGACAGAAGAAATAACGAAAAAATTTGGTGATGAGCGTAAGAAAGCTCTTGATGATGCTTT
GAAAAACATCGAAAAAGATTTTGGTAAAGGTGCAGTTATGCGTCTGGGTGAACGCGCTGAACAAAAGGTTCAGGTGATGA
GTTCAGGAAGTCTGGCTCTTGATATTGCTCTTGGGGCAGGCGGTTATCCAAAAGGGCGTATCGTTGAGATTTACGGGCCA
GAATCTTCTGGTAAGACAACTGTCGCTCTTCATGCTGTTGCTCAGGCGCAAAAAGATGGCGGTATTGCCGCTTTCATTGA
TGCAGAACATGCCCTTGATCCAGCCTATGCTGCTGCTCTTGGTGTTAATATTGATGAGCTTTTGCTTTCACAACCAGATT
CAGGAGAACAGGGTCTTGAAATTGCAGGGAAATTGATTGATTCTGGCGCTGTTGATTTAGTTGTTGTTGACTCAGTGGCA
GCTTTAGTACCACGTGCGGAGATTGACGGAGATATTGGTGATAGTCATGTTGGCTTACAAGCACGCATGATGAGTCAAGC
GATGCGTAAATTATCAGCTTCAATAAATAAAACAAAAACCATTGCCATTTTTATTAATCAATTGCGGGAAAAAGTTGGTA
TTATGTTTGGTAATCCAGAAACAACCCCTGGCGGGCGTGCCTTGAAGTTTTATTCTTCTGTGCGTCTTGATGTCCGTGGC
AATACTCAAATTAAAGGAACCGGGGAACAAAAAGACAGCAATATTGGTAAAGAGACCAAAATTAAAGTTGTTAAAAATAA
AGTTGCTCCACCATTTAAGGAAGCTTTTGTAGAAATTATATATGGTGAAGGCATTTCTCGTACAGGTGAATTAGTTAAGA
TTGCCAGTGATTTGGGAATTATCCAAAAAGCTGGAGCTTGGTACTCTTATAATGGTGAAAAAATTGGGCAAGGTTCTGAA
AATGCTAAAAAATTCCTAGCTGATAATCCAGAAATTTTTGATGACATCGATCATAAAGTGCGTGTTCAATATGGTTTAAT
TGAGGAAGATAATACTGAAGAAAAACAGTCTTCTAAAGAAAAAGAAACTGATGAGAAAGCTGACAAAAACCTTGTTTTAG
AGCTGGATGATACAATTGAGCTTGAAGATTAA

Domains

Predicted by InterproScan.

• recA bacterial DNA recombination protein (RecA) (21-286)

3D structure

• AlphaFold DB: P27624

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
recA	Streptococcus pyogenes NZ131	87.206	100	0.884
recA	Streptococcus pneumoniae Rx1	83.204	100	0.841
recA	Streptococcus pneumoniae D39	83.204	100	0.841
recA	Streptococcus pneumoniae R6	83.204	100	0.841
recA	Streptococcus pneumoniae TIGR4	83.204	100	0.841
recA	Streptococcus mitis NCTC 12261	84.293	99.739	0.841
recA	Streptococcus mitis SK321	83.77	99.739	0.836
recA	Lactococcus lactis subsp. cremoris KW2	72.208	99.483	0.718
recA	Latilactobacillus sakei subsp. sakei 23K	68.208	97.465	0.665
recA	Bacillus subtilis subsp. subtilis str. 168	66.092	100	0.661
recA	Neisseria gonorrhoeae strain FA1090	60.684	100	0.612
recA	Neisseria gonorrhoeae MS11	60.684	100	0.612
recA	Neisseria gonorrhoeae MS11	60.684	100	0.612
recA	Riemerella anatipestifer ATCC 11845 = DSM 15868	60.411	99.417	0.601
recA	Acinetobacter baylyi ADP1	59.375	100	0.599
recA	Acinetobacter baumannii D1279779	61.585	93.983	0.579
recA	Vibrio cholerae strain A1552	61.231	91.808	0.562
recA	Vibrio cholerae O1 biovar El Tor strain E7946	61.231	91.808	0.562
recA	Pseudomonas stutzeri DSM 10701	57.864	97.118	0.562
recA	Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819	57.704	96.501	0.557
recA	Ralstonia pseudosolanacearum GMI1000	59.271	93.466	0.554
recA	Glaesserella parasuis strain SC1401	60.294	91.398	0.551
recA	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	61.92	88.981	0.551
recA	Helicobacter pylori 26695	56.287	96.254	0.542
recA	Helicobacter pylori strain NCTC11637	56.287	96.254	0.542

References

• [1]: R E Eaton et al. (2010) Deletion of competence-induced genes over-expressed in biofilms caused transformation deficiencies in Streptococcus mutans. Molecular Oral Microbiology 25(6):406-17. [PMID: 21040514]