Detailed information    

experimental Experimentally validated

Overview

Name   recA   Type   Machinery gene
Locus tag   RA0C_RS04870 Genome accession   NC_017045
Coordinates   1004444..1005475 (-) Length   343 a.a.
NCBI ID   WP_004918236.1    Uniprot ID   E4TBP8
Organism   Riemerella anatipestifer ATCC 11845 = DSM 15868     
Function   homologous recombination   
Homologous recombination

Function

Among these genes, RA0C_RS04895, RA0C_RS05130, RA0C_RS09020, RA0C_RS02645, RA0C_RS04870, RA0C_RS05105, RA0C_RS04915, and RA0C_RS04920 were shown to be essential for natural transformation occurrence.


Genomic Context

Location: 999444..1010475
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RA0C_RS04855 (RA0C_1015) - 1000121..1000705 (+) 585 WP_013446913.1 ribonuclease HII -
  RA0C_RS04860 (RA0C_1016) yidC 1000809..1002599 (-) 1791 WP_013446914.1 membrane protein insertase YidC -
  RA0C_RS04865 (RA0C_1017) - 1002683..1004299 (-) 1617 WP_013446915.1 CTP synthase -
  RA0C_RS04870 (RA0C_1019) recA 1004444..1005475 (-) 1032 WP_004918236.1 recombinase RecA Machinery gene
  RA0C_RS04875 (RA0C_1020) dinB 1005572..1006654 (-) 1083 WP_013446916.1 DNA polymerase IV -
  RA0C_RS04880 (RA0C_1021) - 1006740..1007510 (-) 771 WP_004918231.1 succinate dehydrogenase/fumarate reductase iron-sulfur subunit -
  RA0C_RS04885 (RA0C_1022) - 1007534..1009546 (-) 2013 WP_013446917.1 fumarate reductase/succinate dehydrogenase flavoprotein subunit -
  RA0C_RS04890 (RA0C_1023) - 1009611..1010279 (-) 669 WP_013446918.1 succinate dehydrogenase cytochrome b subunit -

Sequence

Protein


Download         Length: 343 a.a.        Molecular weight: 36868.17 Da        Isoelectric Point: 6.0810

>NTDB_id=1334 RA0C_RS04870 WP_004918236.1 1004444..1005475(-) (recA) [Riemerella anatipestifer ATCC 11845 = DSM 15868]
MAKTETTSPNDDKKKALAAVLEKLDKTYGKGTVMKLGDGTVDHNIEVIPSGSLGLDLALGVGGYPKGRIVEIYGPESSGK
TTLTLHAIAEAQKVGGTAAFIDAEHAFDMHYAEKLGINLEELIVSQPDNGEQALEIADSLIRSGGVDIVVVDSVAALTPK
AEIDGDMGDSKMGLHARLMSQALRKLTSSISKTKCTVIFINQLRDKIGVMFGSPETTTGGNALKFYASVRIDIRRSLSDT
GKIKDKSGEVIGNRTKVKVVKNKVAPPFKSAEFDIMYGEGVSKVGEILDVATDLDIIQKSGSWFSYQDTKLGQGRDTVKE
LIKDNPELAEELEAKIREKLQAK

Nucleotide


Download         Length: 1032 bp        

>NTDB_id=1334 RA0C_RS04870 WP_004918236.1 1004444..1005475(-) (recA) [Riemerella anatipestifer ATCC 11845 = DSM 15868]
ATGGCAAAGACTGAAACAACAAGTCCTAACGATGATAAAAAAAAGGCATTAGCAGCCGTATTAGAAAAACTAGATAAAAC
TTACGGTAAAGGTACCGTTATGAAACTAGGTGATGGTACGGTAGACCATAATATAGAGGTGATTCCGTCAGGTTCTTTAG
GGCTAGATTTGGCTTTAGGGGTAGGTGGTTATCCTAAGGGGAGAATTGTAGAAATCTACGGTCCAGAATCTTCTGGTAAA
ACTACATTAACGCTTCACGCAATTGCGGAAGCTCAAAAAGTAGGGGGAACAGCAGCATTTATAGATGCAGAACATGCTTT
TGATATGCACTATGCCGAAAAATTAGGCATCAATCTAGAAGAGCTTATCGTGTCTCAACCAGATAATGGAGAGCAGGCAT
TAGAAATTGCAGATAGCCTTATTCGTTCTGGAGGAGTGGATATTGTAGTGGTAGACTCTGTGGCAGCACTTACGCCTAAA
GCAGAGATTGATGGTGATATGGGAGATTCTAAAATGGGTCTTCACGCAAGACTGATGTCTCAAGCACTTAGAAAATTAAC
TTCTTCTATTTCTAAAACAAAATGTACTGTAATTTTCATCAACCAGTTGAGAGATAAAATTGGAGTAATGTTTGGTAGCC
CTGAAACAACAACGGGAGGTAACGCTCTTAAATTCTATGCTTCGGTAAGGATAGATATCAGACGCTCGTTGAGTGATACA
GGTAAGATTAAAGATAAATCTGGCGAAGTTATAGGTAACCGAACTAAAGTAAAAGTGGTTAAAAATAAAGTAGCTCCACC
ATTTAAGAGTGCTGAGTTTGATATTATGTATGGGGAAGGTGTGTCTAAAGTTGGTGAGATTTTAGATGTGGCTACAGATT
TAGATATTATCCAAAAGAGTGGTTCTTGGTTCAGTTATCAGGACACAAAGCTAGGACAAGGGCGTGATACGGTAAAGGAA
CTCATCAAAGATAATCCTGAACTTGCAGAAGAGCTAGAAGCAAAAATCAGAGAAAAATTACAAGCTAAATAG

Domains

Predicted by InterproScan.

recA bacterial DNA recombination protein (RecA)

(15-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E4TBP8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recA Neisseria gonorrhoeae MS11

67.073

95.627

0.641
  recA Neisseria gonorrhoeae strain FA1090

67.073

95.627

0.641
  recA Neisseria gonorrhoeae MS11

67.073

95.627

0.641
  recA Glaesserella parasuis strain SC1401

63.037

100

0.641
  recA Helicobacter pylori strain NCTC11637

64.458

96.793

0.624
  recA Helicobacter pylori 26695

64.458

96.793

0.624
  recA Acinetobacter baylyi ADP1

63.772

97.376

0.621
  recA Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

63.855

96.793

0.618
  recA Acinetobacter baumannii D1279779

63.746

96.501

0.615
  recA Ralstonia pseudosolanacearum GMI1000

64.286

95.455

0.614
  recA Bacillus subtilis subsp. subtilis str. 168

63.609

95.335

0.606
  recA Streptococcus pyogenes NZ131

61.721

98.251

0.606
  recA Streptococcus mutans UA159

60.411

99.417

0.601
  recA Pseudomonas stutzeri DSM 10701

61.377

97.376

0.598
  recA Streptococcus mitis NCTC 12261

62.195

95.627

0.595
  recA Streptococcus mitis SK321

62.195

95.627

0.595
  recA Vibrio cholerae O1 biovar El Tor strain E7946

61.027

96.501

0.589
  recA Vibrio cholerae strain A1552

61.027

96.501

0.589
  recA Streptococcus pneumoniae Rx1

60.725

96.501

0.586
  recA Streptococcus pneumoniae D39

60.725

96.501

0.586
  recA Streptococcus pneumoniae R6

60.725

96.501

0.586
  recA Streptococcus pneumoniae TIGR4

60.725

96.501

0.586
  recA Lactococcus lactis subsp. cremoris KW2

60.486

95.918

0.58
  recA Latilactobacillus sakei subsp. sakei 23K

59.327

95.335

0.566
  recA Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

54.81

100

0.548

Multiple sequence alignment    



References

[1] Li Huang et al. (2021) Identification of the Natural Transformation Genes in Riemerella anatipestifer by Random Transposon Mutagenesis. Frontiers in Microbiology 12:712198. [PMID: 34566918]