Detailed information NTG0001427

Experimentally validated

Overview

• Name: recA
• Type: Machinery gene
• Locus tag: RS_RS02760
• Genome accession: NC_003295
• Coordinates: 596177..597235 (+)
• Length: 352 a.a.
• NCBI ID: WP_011000510.1
• Uniprot ID: A0A223GJI1
• Organism: Ralstonia pseudosolanacearum GMI1000
• Function: homologous recombination Homologous recombination

Function

Our results confirmed the critical role of the comA gene for natural transformation and that of recA for recombination and, more surprisingly, for the maintenance of an autonomous plasmid in the host cell.

Genomic Context

Location: 591177..602235

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RS_RS02740 (RS04919)	-	591452..593155 (-)	1704	WP_011000506.1	MFS transporter	-
RS_RS02745 (RS04918)	-	593354..593671 (-)	318	WP_011000507.1	DUF4212 domain-containing protein	-
RS_RS02750 (RS04917)	-	593668..595209 (-)	1542	WP_011000508.1	sensor histidine kinase	-
RS_RS02755 (RS04916)	-	595202..595900 (-)	699	WP_011000509.1	response regulator	-
RS_RS02760 (RS04915)	recA	596177..597235 (+)	1059	WP_011000510.1	recombinase RecA	Machinery gene
RS_RS02765 (RS04913)	recX	597330..597809 (+)	480	WP_011000511.1	recombination regulator RecX	-
RS_RS02770 (RS04912)	-	598084..598710 (+)	627	WP_011000512.1	DUF2889 domain-containing protein	-
RS_RS02775 (RS04911)	sucC	598777..599943 (+)	1167	WP_011000513.1	ADP-forming succinate--CoA ligase subunit beta	-
RS_RS02780 (RS04910)	sucD	600028..600909 (+)	882	WP_011000514.1	succinate--CoA ligase subunit alpha	-
RS_RS02785 (RS04909)	-	601059..601757 (+)	699	WP_011000515.1	TerC family protein	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 37658.31 Da
• Isoelectric Point: 4.9116

>NTDB_id=1427 RS_RS02760 WP_011000510.1 596177..597235(+) (recA) [Ralstonia pseudosolanacearum GMI1000]
MEDGKKAASMSAEKQKALAAALAQIEKQFGKGSIMKMGDAEVEPVQVVSTGSLGLDVALGVGGLPRGRVVEIYGPESSGK
TTLTLQVVAEMQKLGGTCAFIDAEHALDVTYADKLGVKVPDLLISQPDTGEQALEIADALVRSGSVDLIVIDSVAALVPK
AEIEGEMGDALPGLQARLMSQALRKLTGTIKRTNCLVIFINQIRMKIGVMFGSPETTTGGNALKFYASVRLDIRRIGSIK
KGDEVVGNETKVKVVKNKVAPPFREAIFDILYGAGVSREGEIIDLGVEAKVVEKSGAWYSYNGERIGQGRDNCREFLREN
AELAREIENKVREHLGVTPMGAVTLAEEVEED

Nucleotide

• Length: 1059 bp

>NTDB_id=1427 RS_RS02760 WP_011000510.1 596177..597235(+) (recA) [Ralstonia pseudosolanacearum GMI1000]
ATGGAAGACGGCAAGAAGGCAGCCTCGATGAGCGCAGAAAAGCAGAAGGCGCTGGCTGCCGCGCTCGCGCAGATCGAAAA
GCAGTTCGGCAAGGGCTCGATCATGAAGATGGGCGACGCCGAGGTGGAGCCGGTCCAGGTTGTGTCCACCGGCTCGCTGG
GTCTGGACGTCGCGCTGGGCGTCGGCGGCCTGCCGCGCGGTCGCGTGGTCGAGATCTACGGCCCCGAATCGTCCGGCAAG
ACCACGCTGACGCTGCAGGTGGTCGCCGAGATGCAGAAGCTGGGCGGCACCTGCGCCTTCATCGACGCCGAGCATGCGCT
GGACGTCACCTATGCCGACAAGCTCGGCGTGAAGGTGCCGGACCTGCTGATCTCCCAACCGGACACCGGTGAGCAGGCGC
TGGAAATCGCTGACGCGCTGGTGCGCTCGGGCTCGGTCGACCTGATCGTCATCGACTCGGTGGCCGCGCTGGTGCCCAAG
GCCGAAATCGAAGGCGAGATGGGCGACGCGCTGCCCGGTCTGCAGGCCCGCCTGATGAGCCAGGCGCTGCGCAAGCTGAC
CGGCACCATCAAGCGCACCAACTGCCTGGTGATCTTCATCAACCAGATCCGCATGAAGATCGGCGTGATGTTCGGCTCGC
CGGAAACCACCACGGGCGGCAATGCGCTCAAGTTTTACGCTTCGGTGCGTCTGGACATCCGCCGCATCGGCTCGATCAAG
AAGGGCGATGAGGTGGTCGGCAACGAGACCAAGGTCAAGGTCGTCAAGAACAAGGTGGCGCCGCCGTTCCGCGAGGCGAT
CTTCGACATCCTCTACGGTGCGGGCGTGTCGCGCGAAGGCGAGATCATCGATCTGGGCGTGGAAGCCAAGGTGGTCGAGA
AATCCGGCGCCTGGTACAGCTACAACGGCGAGCGCATCGGTCAGGGCCGTGACAACTGCCGCGAATTCCTGCGCGAAAAC
GCCGAACTGGCCCGCGAGATCGAAAACAAGGTCCGCGAACACCTGGGCGTGACGCCGATGGGCGCCGTCACGCTGGCCGA
GGAAGTCGAGGAAGATTGA

Domains

Predicted by InterproScan.

• recA bacterial DNA recombination protein (RecA) (16-277)

3D structure

• AlphaFold DB: A0A223GJI1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
recA	Pseudomonas stutzeri DSM 10701	72.303	97.443	0.705
recA	Neisseria gonorrhoeae MS11	74.468	94.54	0.704
recA	Neisseria gonorrhoeae MS11	74.468	94.54	0.704
recA	Neisseria gonorrhoeae strain FA1090	74.468	94.54	0.704
recA	Acinetobacter baumannii D1279779	70.058	97.727	0.685
recA	Acinetobacter baylyi ADP1	67.33	100	0.679
recA	Vibrio cholerae O1 biovar El Tor strain E7946	69.822	96.023	0.67
recA	Vibrio cholerae strain A1552	69.822	96.023	0.67
recA	Glaesserella parasuis strain SC1401	70.031	92.898	0.651
recA	Riemerella anatipestifer ATCC 11845 = DSM 15868	64.286	95.455	0.614
recA	Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819	62.974	97.443	0.614
recA	Bacillus subtilis subsp. subtilis str. 168	64.742	94.54	0.612
recA	Helicobacter pylori 26695	62.202	96.83	0.602
recA	Helicobacter pylori strain NCTC11637	61.905	96.83	0.599
recA	Latilactobacillus sakei subsp. sakei 23K	63.804	92.614	0.591
recA	Lactococcus lactis subsp. cremoris KW2	60.303	93.75	0.565
recA	Streptococcus pneumoniae Rx1	59.697	93.75	0.56
recA	Streptococcus pneumoniae R6	59.697	93.75	0.56
recA	Streptococcus pneumoniae D39	59.697	93.75	0.56
recA	Streptococcus pneumoniae TIGR4	59.697	93.75	0.56
recA	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	60.123	92.614	0.557
recA	Streptococcus mitis NCTC 12261	59.394	93.75	0.557
recA	Streptococcus mitis SK321	59.394	93.75	0.557
recA	Streptococcus mutans UA159	59.271	93.466	0.554
recA	Streptococcus pyogenes NZ131	58.537	93.182	0.545

References

• [1]: Anne Mercier et al. (2009) Influence of DNA conformation and role of comA and recA on natural transformation in Ralstonia solanacearum. Canadian Journal of Microbiology 55(6):762-70. [PMID: 19767847]