Detailed information NTG0000625

Experimentally validated

Overview

• Name: recA
• Type: Machinery gene
• Locus tag: LCA_RS02525
• Genome accession: NC_007576
• Coordinates: 504928..505995 (+)
• Length: 355 a.a.
• NCBI ID: WP_011374196.1
• Uniprot ID: Q38YD9
• Organism: Latilactobacillus sakei subsp. sakei 23K
• Function: homologous recombination Homologous recombination

Function

Whereas recA was little activated, expression of dprA was highly induced in the sigH(hy)* context.

Genomic Context

Location: 499928..510995

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LCA_RS02505 (LCA_0483)	yfmH	501095..502399 (+)	1305	WP_011374192.1	EF-P 5-aminopentanol modification-associated protein YfmH	-
LCA_RS02510 (LCA_0484)	ymfI	502399..503130 (+)	732	WP_011374193.1	elongation factor P 5-aminopentanone reductase	-
LCA_RS02515 (LCA_0485)	-	503212..504135 (+)	924	WP_011374194.1	helix-turn-helix domain-containing protein	-
LCA_RS02520 (LCA_0486)	pgsA	504161..504745 (+)	585	WP_011374195.1	CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase	-
LCA_RS02525 (LCA_0487)	recA	504928..505995 (+)	1068	WP_011374196.1	recombinase RecA	Machinery gene
LCA_RS02530 (LCA_0489)	rny	506382..507947 (+)	1566	WP_011374197.1	ribonuclease Y	-
LCA_RS02535 (LCA_0490)	-	508103..509191 (+)	1089	WP_041821010.1	glycosyltransferase family 4 protein	-
LCA_RS02540 (LCA_0491)	-	509218..509883 (-)	666	WP_011374199.1	YigZ family protein	-

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
sigH	recA	positive effect
sigH	comEC	positive effect
sigH	comFC	positive effect
sigH	comGE	positive effect
sigH	comGA	positive effect
sigH	ssb	positive effect
sigH	comEA	positive effect
sigH	comFA	positive effect
sigH	comGB	positive effect
sigH	comGC	positive effect
sigH	dprA	positive effect
sigH	comGD	positive effect
sigH	comGF	positive effect
sigH	comC	positive effect

Sequence

Protein

• Length: 355 a.a.
• Molecular weight: 38254.25 Da
• Isoelectric Point: 4.6316

>NTDB_id=625 LCA_RS02525 WP_011374196.1 504928..505995(+) (recA) [Latilactobacillus sakei subsp. sakei 23K]
MAKDERQAALDAALKKIEKNFGKGSIMRMGEKVDTQVSTVSSGSLALDEALGVGGYPRGRIVEIYGPESSGKTTVALHAV
AEVQKQGGTAAYIDAENAMDPKYATALGVNIDDLLLSQPDTGEQGLEIADALVSSGAVDILVVDSVAALVPRAEIEGEMG
DAHVGLQARLMSQALRKLSGTINKTKTIALFINQIREKVGVMFGNPEVTPGGRALKFYSTVRLEVRRAETIKNGTDMIGN
RARIKVVKNKVAPPFKVAEVDIMYGQGISRTGELVDMAVEKDIINKSGSWYSYGSERIGQGRENAKNYLADHEDVEDEVR
LKVRAAYGISDVPEEDLPTTEDEQINILPDDSTEE

Nucleotide

• Length: 1068 bp

>NTDB_id=625 LCA_RS02525 WP_011374196.1 504928..505995(+) (recA) [Latilactobacillus sakei subsp. sakei 23K]
TTGGCTAAAGATGAAAGACAAGCAGCACTTGATGCTGCCCTAAAGAAGATTGAAAAGAATTTTGGTAAAGGTTCAATTAT
GCGAATGGGTGAAAAGGTTGATACACAAGTCTCAACAGTTTCATCTGGTTCATTGGCATTGGACGAAGCGCTCGGAGTAG
GTGGTTATCCTCGCGGCCGGATCGTTGAAATTTACGGTCCTGAAAGTTCAGGTAAAACAACCGTTGCGTTACATGCTGTT
GCAGAAGTGCAAAAGCAAGGTGGAACGGCTGCTTATATCGATGCTGAAAATGCGATGGATCCTAAGTATGCAACAGCACT
TGGCGTTAATATTGATGACTTACTCTTGTCACAACCTGATACTGGTGAACAAGGCTTAGAAATTGCTGATGCCTTGGTAT
CAAGTGGGGCCGTTGATATTTTAGTTGTCGATTCAGTTGCTGCTTTGGTACCACGTGCTGAAATCGAAGGCGAAATGGGT
GACGCCCACGTTGGCTTACAAGCCCGTTTGATGTCACAAGCCTTACGTAAATTATCAGGGACGATTAATAAGACGAAGAC
GATTGCGTTATTCATTAACCAAATTCGTGAAAAAGTCGGCGTGATGTTTGGTAACCCCGAAGTCACACCAGGTGGTCGTG
CTTTGAAGTTCTACTCAACTGTTCGGCTTGAAGTGCGTCGTGCTGAAACCATCAAAAATGGGACAGATATGATTGGGAAC
CGGGCCCGAATCAAAGTCGTTAAGAATAAGGTTGCGCCACCATTTAAGGTCGCTGAAGTTGATATCATGTACGGTCAAGG
GATTTCCAGAACCGGTGAATTGGTTGATATGGCGGTCGAAAAAGACATTATCAATAAGAGTGGTTCTTGGTACTCTTATG
GCTCAGAACGAATTGGCCAAGGGCGAGAAAATGCTAAGAACTATTTGGCTGATCACGAAGATGTCGAAGATGAAGTTCGT
TTGAAGGTCAGAGCAGCTTATGGTATTTCAGATGTACCTGAAGAAGATCTCCCAACAACTGAAGATGAACAAATAAATAT
TTTACCAGATGATTCAACAGAAGAATAA

Domains

Predicted by InterProScan.

• recA bacterial DNA recombination protein (RecA_N) (9-269)

3D structure

• AlphaFold DB: Q38YD9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
recA	Bacillus subtilis subsp. subtilis str. 168	74.924	93.966	0.704
recA	Staphylococcus aureus strain ATCC 12600	70.206	97.695	0.686
recA	Streptococcus thermophilus LMD-9	66.575	100	0.679
recA	Streptococcus mutans UA159	68.208	97.465	0.665
recA	Streptococcus pyogenes NZ131	71.429	92.676	0.662
recA	Streptococcus thermophilus LMG 18311	67.919	97.465	0.662
recA	Streptococcus pneumoniae R36A	67.919	97.465	0.662
recA	Streptococcus pneumoniae Rx1	67.919	97.465	0.662
recA	Streptococcus pneumoniae R6	67.919	97.465	0.662
recA	Streptococcus pneumoniae D39	67.919	97.465	0.662
recA	Streptococcus pneumoniae TIGR4	67.919	97.465	0.662
recA	Lactococcus lactis subsp. cremoris KW2	70.359	94.085	0.662
recA	Streptococcus mitis SK321	68.222	96.62	0.659
recA	Streptococcus mitis NCTC 12261	69.486	93.239	0.648
recA	Ralstonia pseudosolanacearum GMI1000	63.804	92.614	0.591
recA	Neisseria gonorrhoeae strain FA1090	62.691	93.966	0.589
recA	Neisseria gonorrhoeae MS11	62.691	93.966	0.589
recA	Riemerella anatipestifer ATCC 11845 = DSM 15868	59.327	95.335	0.566
recA	Helicobacter pylori 26695	54.902	100	0.565
recA	Helicobacter pylori strain NCTC11637	54.902	100	0.565
recA	Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819	59.021	95.335	0.563
recA	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	61.61	90.986	0.561
recA	Pseudomonas stutzeri DSM 10701	57.227	97.695	0.559
recA	Vibrio cholerae O1 biovar El Tor strain E7946	60.748	90.678	0.551
recA	Vibrio cholerae strain A1552	60.748	90.678	0.551
recA	Acinetobacter baylyi ADP1	59.19	91.977	0.544
recA	Acinetobacter baumannii D1279779	58.567	91.977	0.539
recA	Glaesserella parasuis strain SC1401	59.19	90.423	0.535
recA	Acinetobacter nosocomialis M2	58.255	91.714	0.534

References

• [1]: Solveig Schmid et al. (2012) Alternative sigma factor σH activates competence gene expression in Lactobacillus sakei. BMC Microbiology 12:32. [PMID: 22409597]