Detailed information NTG0000624

Experimentally validated

Overview

• Name: ssb
• Type: Machinery gene
• Locus tag: LCA_RS00040
• Genome accession: NC_007576
• Coordinates: 9683..10195 (+)
• Length: 170 a.a.
• NCBI ID: WP_011373726.1
• Uniprot ID: A0A094Y4Q3
• Organism: Latilactobacillus sakei subsp. sakei 23K
• Function: ssDNA binding DNA processing

Function

In contrast, transcription of ssb, regulated as a late competence gene in B. subtilis, was nearly constant or only very weakly induced.

Genomic Context

Location: 4683..15195

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LCA_RS00030 (LCA_0006)	gyrA	6538..9111 (+)	2574	WP_011373724.1	DNA gyrase subunit A	-
LCA_RS00035 (LCA_0007)	rpsF	9348..9644 (+)	297	WP_011373725.1	30S ribosomal protein S6	-
LCA_RS00040 (LCA_0008)	ssb	9683..10195 (+)	513	WP_011373726.1	single-stranded DNA-binding protein	Machinery gene
LCA_RS00045 (LCA_0009)	rpsR	10226..10465 (+)	240	WP_004265822.1	30S ribosomal protein S18	-
LCA_RS00050 (LCA_0010)	-	10683..12716 (+)	2034	WP_011373727.1	DHH family phosphoesterase	-
LCA_RS00055 (LCA_0011)	rplI	12737..13189 (+)	453	WP_011373728.1	50S ribosomal protein L9	-
LCA_RS00060 (LCA_0012)	dnaB	13320..14717 (+)	1398	WP_041820662.1	replicative DNA helicase	-

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
sigH	ssb	positive effect
sigH	comEC	positive effect
sigH	comFC	positive effect
sigH	comGE	positive effect
sigH	recA	positive effect
sigH	comGA	positive effect
sigH	comEA	positive effect
sigH	comFA	positive effect
sigH	comGB	positive effect
sigH	comGC	positive effect
sigH	dprA	positive effect
sigH	comGD	positive effect
sigH	comGF	positive effect
sigH	comC	positive effect

Sequence

Protein

• Length: 170 a.a.
• Molecular weight: 18610.19 Da
• Isoelectric Point: 4.6856

>NTDB_id=624 LCA_RS00040 WP_011373726.1 9683..10195(+) (ssb) [Latilactobacillus sakei subsp. sakei 23K]
MINRVVLVGRLTRDVDLRYTSSGAAVGTFSMAVNRQFTNANGDREADFINCVIWRKSAENFANFTKKGSLVGVDGRLQTR
NYENQQGQRVYVTEVVVDNFSLLESRTTTEQRQGDGASQNFNSNQSNGSQQSGFTSPQQTGNAPAANNTQADPFANNGQA
IDISDDDLPF

Nucleotide

• Length: 513 bp

>NTDB_id=624 LCA_RS00040 WP_011373726.1 9683..10195(+) (ssb) [Latilactobacillus sakei subsp. sakei 23K]
ATGATTAATCGAGTTGTACTCGTTGGTCGGCTAACACGCGATGTTGACTTACGTTACACTTCTAGTGGTGCTGCAGTTGG
GACTTTCTCAATGGCAGTTAACCGTCAATTCACGAACGCTAACGGCGATCGTGAAGCAGACTTTATTAATTGTGTCATTT
GGCGTAAATCAGCGGAAAATTTCGCTAACTTTACTAAAAAGGGCTCATTAGTAGGGGTTGATGGCCGTCTACAAACTAGA
AATTATGAAAATCAACAAGGTCAACGTGTCTACGTGACTGAAGTGGTTGTCGATAATTTCTCATTGTTAGAATCACGGAC
AACAACAGAGCAACGGCAAGGTGATGGCGCAAGTCAGAACTTTAATAGTAATCAAAGCAATGGTAGCCAACAATCTGGAT
TTACCAGCCCTCAACAAACGGGTAACGCACCAGCCGCAAATAACACTCAAGCCGATCCATTTGCAAATAATGGTCAAGCG
ATTGATATTTCGGATGATGACTTACCTTTCTAA

Domains

Predicted by InterproScan.

• Single-strand binding protein family (SSB) (2-102)

3D structure

• AlphaFold DB: A0A094Y4Q3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ssbA	Bacillus subtilis subsp. subtilis str. 168	58.192	100	0.599
ssbB	Bacillus subtilis subsp. subtilis str. 168	59.434	93.805	0.558
ssbB/cilA	Streptococcus mitis NCTC 12261	47.788	86.26	0.412
ssbB/cilA	Streptococcus pneumoniae TIGR4	47.788	86.26	0.412
ssbB	Streptococcus sobrinus strain NIDR 6715-7	45.763	90.076	0.412
ssbB/cilA	Streptococcus pneumoniae R6	46.903	86.26	0.405
ssbB/cilA	Streptococcus mitis SK321	46.903	86.26	0.405
ssbB/cilA	Streptococcus pneumoniae D39	46.903	86.26	0.405
ssbB/cilA	Streptococcus pneumoniae Rx1	46.903	86.26	0.405
ssb	Glaesserella parasuis strain SC1401	33.69	100	0.371
ssbB	Lactococcus lactis subsp. cremoris KW2	41.964	86.822	0.364

References

• [1]: Solveig Schmid et al. (2012) Alternative sigma factor σH activates competence gene expression in Lactobacillus sakei. BMC Microbiology 12:32. [PMID: 22409597]