Detailed information    

experimental Experimentally validated

Overview

Name   ssb   Type   Machinery gene
Locus tag   A4U84_RS00055 Genome accession   NZ_CP015099
Coordinates   13391..13933 (-) Length   180 a.a.
NCBI ID   WP_021115958.1    Uniprot ID   A0A836YZ57
Organism   Glaesserella parasuis strain SC1401     
Function   ssDNA binding   
DNA processing

Function

The expression levels of candidate uptake components were significantly lower than in SC1401, respectively. These results revealed that the highly naturally competent strain SC1401 up-regulated most of the candidate natural competence-related regulons/components.


Genomic Context

Location: 8391..18933
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4U84_RS00030 (A4U84_00030) argF 8729..9739 (-) 1011 WP_038513972.1 ornithine carbamoyltransferase -
  A4U84_RS00035 (A4U84_00035) - 9888..10166 (+) 279 WP_005712614.1 type II toxin-antitoxin system RelE/ParE family toxin -
  A4U84_RS00040 (A4U84_00040) - 10177..10449 (+) 273 WP_005712616.1 HigA family addiction module antitoxin -
  A4U84_RS00045 (A4U84_00045) rhlB 10513..11733 (+) 1221 WP_021112303.1 ATP-dependent RNA helicase RhlB -
  A4U84_RS00050 (A4U84_00050) - 11743..13230 (+) 1488 WP_062923800.1 helicase HerA-like C-terminal domain-containing protein -
  A4U84_RS00055 (A4U84_00055) ssb 13391..13933 (-) 543 WP_021115958.1 single-stranded DNA-binding protein Machinery gene
  A4U84_RS00060 (A4U84_00060) uvrA 14236..17064 (+) 2829 WP_062923801.1 excinuclease ABC subunit UvrA -
  A4U84_RS00065 (A4U84_00065) - 17148..17528 (+) 381 WP_010786782.1 YacL family protein -

Sequence

Protein


Download         Length: 180 a.a.        Molecular weight: 19938.78 Da        Isoelectric Point: 4.9833

>NTDB_id=1390 A4U84_RS00055 WP_021115958.1 13391..13933(-) (ssb) [Glaesserella parasuis strain SC1401]
MAGVNKVIIVGNLGNDPDMRTMPNGDAVATLSVATSESWNDKMTGERREVTEWHRIVFFRRQAEVAGQYLRKGSKVYVEG
KLKTRKWQDQNGQDRYTTEIQGDVLQMLDSRSSGGDFGGNQGSGWNQAPAQTNYNQGGYSDNYAQNNNFNGGNATRPQPA
QKPAAQAEPPMDNFDDDIPF

Nucleotide


Download         Length: 543 bp        

>NTDB_id=1390 A4U84_RS00055 WP_021115958.1 13391..13933(-) (ssb) [Glaesserella parasuis strain SC1401]
ATGGCAGGTGTAAATAAAGTAATTATTGTCGGCAATCTCGGTAATGACCCTGATATGCGTACAATGCCAAACGGCGATGC
GGTTGCAACATTAAGCGTAGCAACCAGTGAAAGTTGGAACGATAAAATGACAGGCGAACGCCGTGAGGTCACCGAATGGC
ACCGTATCGTTTTCTTCCGTCGTCAAGCAGAAGTAGCAGGACAATACTTACGCAAAGGTTCAAAAGTTTATGTAGAAGGC
AAGCTCAAAACACGCAAATGGCAAGATCAAAACGGTCAAGATCGTTACACCACAGAAATTCAAGGCGATGTACTACAAAT
GTTAGACAGCCGTAGCAGTGGCGGTGATTTTGGCGGAAATCAAGGTAGCGGTTGGAACCAAGCACCAGCGCAAACCAACT
ACAATCAAGGCGGCTACAGCGACAACTATGCTCAAAATAACAACTTCAATGGGGGCAACGCAACCAGACCGCAACCAGCC
CAAAAACCTGCGGCTCAAGCAGAACCACCAATGGATAATTTTGATGATGATATTCCATTTTAA

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(4-108)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A836YZ57

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Vibrio cholerae strain A1552

50.265

100

0.537
  ssb Neisseria gonorrhoeae MS11

44.505

100

0.466
  ssb Neisseria meningitidis MC58

43.956

100

0.46
  ssbA Bacillus subtilis subsp. subtilis str. 168

35.519

100

0.378
  ssb Latilactobacillus sakei subsp. sakei 23K

33.69

100

0.371

Multiple sequence alignment    



References

[1] Ke Dai et al. (2018) Basic Characterization of Natural Transformation in a Highly Transformable Haemophilus parasuis Strain SC1401. Frontiers in Cellular And Infection Microbiology 8:32. [PMID: 29473023]