Detailed information    

experimental Experimentally validated

Overview

Name   ssb   Type   Machinery gene
Locus tag   NMB_RS07590 Genome accession   NC_003112
Coordinates   1509486..1510010 (-) Length   174 a.a.
NCBI ID   WP_002212976.1    Uniprot ID   P66849
Organism   Neisseria meningitidis MC58     
Function   ssDNA binding   
DNA processing

Function

The ComL DNA binding properties and outer membrane localization suggest that this lipoprotein plays a direct role in neisserial transformation, while neisserial SSB is a DNA binding protein that contributes to the terminal part of the transformation process.


Genomic Context

Location: 1504486..1515010
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMB_RS07580 (NMB1458) fumC 1506585..1507973 (+) 1389 WP_002225106.1 class II fumarate hydratase -
  NMB_RS07585 (NMB1459) - 1508038..1508961 (-) 924 WP_002225105.1 DMT family transporter -
  NMB_RS07590 (NMB1460) ssb 1509486..1510010 (-) 525 WP_002212976.1 single-stranded DNA-binding protein Machinery gene
  NMB_RS07595 (NMB1461) - 1510014..1511396 (-) 1383 WP_002225104.1 MFS transporter -
  NMB_RS07600 (NMB1462) - 1511507..1512130 (-) 624 WP_002219051.1 lytic transglycosylase domain-containing protein -
  NMB_RS11525 - 1512189..1512410 (+) 222 WP_009347394.1 hypothetical protein -
  NMB_RS11755 (NMB1463) - 1512631..1513039 (-) 409 Protein_1484 hypothetical protein -
  NMB_RS07620 (NMB1464) - 1513088..1513579 (-) 492 WP_002219860.1 REP-associated tyrosine transposase -

Sequence

Protein


Download         Length: 174 a.a.        Molecular weight: 19452.65 Da        Isoelectric Point: 5.8354

>NTDB_id=1103 NMB_RS07590 WP_002212976.1 1509486..1510010(-) (ssb) [Neisseria meningitidis MC58]
MSLNKVILIGRLGRDPEVRYMPNGEAVCNFSVATSETWNDRNGQRVERTEWHNITMYRKLAEIAGQYLKKGGLVYLEGRI
QSRKYQGKDGIERTAYDIVANEMKMLGGRNENSGGAPYEEGYGQSQEAYQRPAQQSRQPASDAPSHPQEAPAAPRRQPVP
AAAPVEDIDDDIPF

Nucleotide


Download         Length: 525 bp        

>NTDB_id=1103 NMB_RS07590 WP_002212976.1 1509486..1510010(-) (ssb) [Neisseria meningitidis MC58]
ATGTCATTGAACAAAGTCATCCTCATCGGCCGCCTCGGACGCGATCCCGAAGTGCGCTATATGCCCAACGGCGAGGCGGT
TTGTAATTTCAGCGTCGCCACCAGCGAAACTTGGAACGACCGCAACGGCCAACGTGTAGAGCGTACTGAGTGGCACAACA
TCACCATGTACCGCAAACTGGCGGAAATTGCCGGGCAATACCTCAAAAAAGGCGGGCTGGTTTATTTGGAAGGCAGAATC
CAAAGCCGCAAATACCAAGGCAAAGACGGCATCGAACGCACCGCTTACGATATTGTCGCCAACGAAATGAAAATGTTGGG
CGGGCGCAATGAAAACAGCGGCGGTGCGCCTTACGAGGAAGGTTACGGTCAGAGTCAGGAGGCTTACCAACGCCCCGCGC
AGCAAAGCCGGCAGCCCGCCTCCGACGCGCCGTCCCATCCCCAAGAAGCACCAGCCGCGCCGCGCCGCCAACCCGTGCCT
GCCGCCGCCCCGGTCGAGGACATTGACGACGATATCCCGTTCTGA

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(3-106)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P66849

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Neisseria gonorrhoeae MS11

98.851

100

0.989
  ssb Vibrio cholerae strain A1552

47.778

100

0.494
  ssb Glaesserella parasuis strain SC1401

43.956

100

0.46

Multiple sequence alignment    



References

[1] Afsaneh V Benam et al. (2011) Structure-function relationships of the competence lipoprotein ComL and SSB in meningococcal transformation. Microbiology (Reading, England) 157(Pt 5):1329-1342. [PMID: 21330432]