Detailed information    

experimental Experimentally validated

Overview

Name   ssb   Type   Machinery gene
Locus tag   A1552VC_RS00795 Genome accession   NZ_CP028894
Coordinates   150856..151389 (+) Length   177 a.a.
NCBI ID   WP_000168289.1    Uniprot ID   Q9KUW2
Organism   Vibrio cholerae strain A1552     
Function   ssDNA binding   
DNA processing

Function

Once inside the cytoplasm, Ssb and DprA bind the ssDNA and facilitate loading of RecA, which ultimately catalyzes recombination with the bacterial chromosome.


Genomic Context

Location: 145856..156389
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A1552VC_RS00780 (A1552VC_00151) uvrA 145897..148719 (-) 2823 WP_000357696.1 excinuclease ABC subunit UvrA -
  A1552VC_RS00785 (A1552VC_00152) galU 148875..149747 (-) 873 WP_001920788.1 UTP--glucose-1-phosphate uridylyltransferase GalU -
  A1552VC_RS00790 (A1552VC_00153) qstR 149920..150564 (-) 645 WP_001188316.1 LuxR C-terminal-related transcriptional regulator Regulator
  A1552VC_RS00795 (A1552VC_00154) ssb 150856..151389 (+) 534 WP_000168289.1 single-stranded DNA-binding protein Machinery gene
  A1552VC_RS00800 (A1552VC_00155) csrD 151513..153525 (+) 2013 WP_001918202.1 RNase E specificity factor CsrD -
  A1552VC_RS00805 (A1552VC_00156) - 153538..154977 (+) 1440 WP_000743253.1 PilN domain-containing protein -
  A1552VC_RS00810 (A1552VC_00157) gspM 154974..155624 (+) 651 WP_000835144.1 type II secretion system protein GspM -
  A1552VC_RS00815 (A1552VC_00158) - 155617..155934 (+) 318 WP_000252812.1 MSHA biogenesis protein MshK -

Sequence

Protein


Download         Length: 177 a.a.        Molecular weight: 19672.90 Da        Isoelectric Point: 5.7626

>NTDB_id=1166 A1552VC_RS00795 WP_000168289.1 150856..151389(+) (ssb) [Vibrio cholerae strain A1552]
MASRGVNKVILIGNLGQDPEVRYMPSGGAVANITIATSETWRDKATGEQKEKTEWHRVTLYGKLAEVAGEYLRKGSQVYI
EGQLQTRKWQDQSGQDRYSTEVVVQGYNGIMQMLGGRAQQGGMPAQGGMNVPAQQGSWGQPQQPAKQHQPMQQSAPQQYS
QPQYNEPPMDFDDDIPF

Nucleotide


Download         Length: 534 bp        

>NTDB_id=1166 A1552VC_RS00795 WP_000168289.1 150856..151389(+) (ssb) [Vibrio cholerae strain A1552]
ATGGCAAGCCGTGGCGTGAACAAAGTAATTTTGATTGGTAACTTAGGCCAAGATCCTGAAGTGCGTTATATGCCAAGCGG
CGGCGCAGTAGCGAATATCACCATCGCGACCTCCGAAACCTGGCGTGATAAAGCCACAGGTGAACAGAAGGAAAAAACCG
AATGGCACCGAGTTACTCTGTATGGAAAATTGGCAGAAGTGGCTGGTGAATATTTGCGTAAAGGTTCTCAAGTTTACATT
GAAGGCCAACTGCAAACGCGTAAGTGGCAAGATCAAAGTGGTCAAGATCGCTACTCAACCGAAGTGGTTGTACAAGGCTA
TAACGGTATCATGCAGATGCTGGGTGGACGTGCACAGCAAGGTGGAATGCCAGCCCAAGGCGGCATGAATGTTCCTGCGC
AGCAAGGAAGTTGGGGACAACCACAACAACCGGCGAAGCAGCATCAGCCTATGCAACAATCTGCGCCTCAACAGTACTCG
CAACCGCAGTATAATGAGCCGCCGATGGATTTTGATGACGACATCCCGTTTTAG

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(6-105)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9KUW2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Glaesserella parasuis strain SC1401

50.265

100

0.537
  ssb Neisseria gonorrhoeae MS11

47.778

100

0.494
  ssb Neisseria meningitidis MC58

47.778

100

0.494

Multiple sequence alignment    



References

[1] Patrick Seitz et al. (2013) DNA-uptake machinery of naturally competent Vibrio cholerae. Proceedings of The National Academy of Sciences of The United States of America 110(44):17987-92. [PMID: 24127573]