Detailed information    

experimental Experimentally validated

Overview

Name   qstR   Type   Regulator
Locus tag   A1552VC_RS00790 Genome accession   NZ_CP028894
Coordinates   149920..150564 (-) Length   214 a.a.
NCBI ID   WP_001188316.1    Uniprot ID   Q9KUW3
Organism   Vibrio cholerae strain A1552     
Function   promote expression of competence genes   
Competence regulation

Function

QstR is required for the proper expression of a small but essential subset of competence genes and propose a new regulatory model in which QstR links chitin-induced TfoX activity with QS.


Genomic Context

Location: 144920..155564
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A1552VC_RS00780 (A1552VC_00151) uvrA 145897..148719 (-) 2823 WP_000357696.1 excinuclease ABC subunit UvrA -
  A1552VC_RS00785 (A1552VC_00152) galU 148875..149747 (-) 873 WP_001920788.1 UTP--glucose-1-phosphate uridylyltransferase GalU -
  A1552VC_RS00790 (A1552VC_00153) qstR 149920..150564 (-) 645 WP_001188316.1 LuxR C-terminal-related transcriptional regulator Regulator
  A1552VC_RS00795 (A1552VC_00154) ssb 150856..151389 (+) 534 WP_000168289.1 single-stranded DNA-binding protein Machinery gene
  A1552VC_RS00800 (A1552VC_00155) csrD 151513..153525 (+) 2013 WP_001918202.1 RNase E specificity factor CsrD -
  A1552VC_RS00805 (A1552VC_00156) - 153538..154977 (+) 1440 WP_000743253.1 PilN domain-containing protein -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  qstR comEC positive effect
  qstR comF positive effect
  qstR comEA positive effect
  qstR comM positive effect
  crp qstR positive effect
  tfoX qstR positive effect
  hapR qstR positive effect
  tfoX comEC positive effect
  tfoX pilO positive effect
  tfoX pilB positive effect
  tfoX pilP positive effect
  tfoX pilA positive effect
  tfoX pilD positive effect
  tfoX dprA positive effect
  tfoX pilM positive effect
  tfoX pilN positive effect
  tfoX comEA positive effect
  tfoX pilQ positive effect
  tfoX pilC positive effect
  chiS tfoX positive effect
  hapR comEC positive effect
  hapR comEA positive effect
  hapR dns negative effect
  crp comEA positive effect

Sequence

Protein


Download         Length: 214 a.a.        Molecular weight: 24736.58 Da        Isoelectric Point: 9.6461

>NTDB_id=1146 A1552VC_RS00790 WP_001188316.1 149920..150564(-) (qstR) [Vibrio cholerae strain A1552]
MQRANYARTIYLLTTQPKALHPSIQAAIEQLNLPVPVIEPERLLREYQSDKHKILLLDHAENGLIRQQLGPLKLTSPYFE
TILFNVEKRLRTEDLLTFGNLKGLFYANEDTGFIAHGLGEIINGQNWLPRHVSSQLLHYYRYAFQSHQVQATVDLTAREI
QILRCLQTGASNMQIAENLFISEFTVKSHLYQIFKKLNVKNRVKAIAWVNQNLL

Nucleotide


Download         Length: 645 bp        

>NTDB_id=1146 A1552VC_RS00790 WP_001188316.1 149920..150564(-) (qstR) [Vibrio cholerae strain A1552]
ATGCAACGAGCCAACTATGCACGCACGATTTATTTATTAACGACACAGCCAAAAGCGTTACATCCCAGTATTCAAGCCGC
CATTGAGCAGTTGAATCTGCCCGTCCCGGTGATTGAACCAGAACGCTTATTGCGCGAGTATCAATCCGATAAACACAAAA
TATTGTTGCTCGACCATGCGGAAAATGGCCTGATTCGCCAGCAATTGGGTCCCCTAAAGCTTACCAGCCCGTACTTCGAA
ACCATCTTGTTTAATGTCGAGAAACGGTTAAGAACAGAAGATCTGCTCACTTTTGGCAACCTCAAAGGTCTGTTTTATGC
CAATGAAGACACAGGCTTTATTGCCCACGGTCTTGGGGAGATCATTAATGGCCAAAACTGGCTACCTCGCCATGTCAGCA
GTCAGTTGCTGCATTACTATCGTTACGCTTTCCAAAGCCATCAAGTTCAAGCCACGGTCGATTTGACTGCACGTGAAATT
CAGATCCTGCGCTGTTTACAAACTGGCGCATCGAATATGCAAATCGCTGAGAATTTATTTATTAGTGAGTTCACGGTGAA
ATCGCATCTTTATCAGATATTTAAAAAGCTAAACGTGAAAAATCGAGTCAAAGCGATCGCTTGGGTTAATCAAAATTTAC
TGTAA

Domains

Predicted by InterproScan.

Bacterial regulatory proteins, luxR family (GerE)

(155-208)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9KUW3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  qstR Vibrio parahaemolyticus RIMD 2210633

52.093

100

0.523
  qstR Vibrio campbellii strain DS40M4

52.336

100

0.523

Multiple sequence alignment    



References

[1] Milena Jaskólska et al. (2018) QstR-dependent regulation of natural competence and type VI secretion in Vibrio cholerae. Nucleic Acids Research 46(20):10619-10634. [PMID: 30102403]
[2] Mirella Lo Scrudato et al. (2014) Regulatory elements involved in the expression of competence genes in naturally transformable Vibrio cholerae. BMC Microbiology 14:327. [PMID: 25539806]
[3] Mirella Lo Scrudato et al. (2013) A transcriptional regulator linking quorum sensing and chitin induction to render Vibrio cholerae naturally transformable. Nucleic Acids Research 41(6):3644-58. [PMID: 23382174]