Detailed information    

experimental Experimentally validated

Overview

Name   pilQ   Type   Machinery gene
Locus tag   A1552VC_RS12105 Genome accession   NZ_CP028894
Coordinates   2577285..2579000 (-) Length   571 a.a.
NCBI ID   WP_000788426.1    Uniprot ID   A0A0K9UP29
Organism   Vibrio cholerae strain A1552     
Function   assembly of type IV pilus   
DNA binding and uptake

Function

No donor DNA was taken up in a strain devoid of the outer membrane secretin PilQ. Piliated cells mostly contain a single pilus that is not biased toward a polar localization and that this pilus colocalizes with the outer membrane secretin PilQ


Genomic Context

Location: 2572285..2584000
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A1552VC_RS12085 (A1552VC_02386) - 2573028..2573861 (-) 834 WP_000744680.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  A1552VC_RS12090 (A1552VC_02387) - 2573931..2575406 (-) 1476 WP_000050195.1 ExeA family protein -
  A1552VC_RS12095 (A1552VC_02388) aroB 2575461..2576546 (-) 1086 WP_000439805.1 3-dehydroquinate synthase -
  A1552VC_RS12100 (A1552VC_02389) aroK 2576574..2577098 (-) 525 WP_000818616.1 shikimate kinase AroK -
  A1552VC_RS12105 (A1552VC_02390) pilQ 2577285..2579000 (-) 1716 WP_000788426.1 type IV pilus secretin PilQ family protein Machinery gene
  A1552VC_RS12110 (A1552VC_02391) pilP 2579026..2579544 (-) 519 WP_000792032.1 pilus assembly protein PilP Machinery gene
  A1552VC_RS12115 (A1552VC_02392) pilO 2579534..2580124 (-) 591 WP_000157690.1 type 4a pilus biogenesis protein PilO Machinery gene
  A1552VC_RS12120 (A1552VC_02393) pilN 2580117..2580701 (-) 585 WP_000902745.1 PilN domain-containing protein Machinery gene
  A1552VC_RS12125 (A1552VC_02394) pilM 2580691..2581707 (-) 1017 WP_000520444.1 type IV pilus assembly protein PilM Machinery gene

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  tfoX pilQ positive effect
  tfoX pilO positive effect
  tfoX pilB positive effect
  tfoX pilP positive effect
  tfoX pilA positive effect
  tfoX pilD positive effect
  tfoX dprA positive effect
  tfoX pilM positive effect
  tfoX pilN positive effect
  tfoX comEA positive effect
  hapR comEA positive effect
  crp comEA positive effect
  qstR comEA positive effect
  tfoX qstR positive effect
  qstR comEC positive effect
  qstR comF positive effect
  qstR comM positive effect
  crp qstR positive effect
  hapR qstR positive effect
  tfoX comEC positive effect
  hapR comEC positive effect
  tfoX pilC positive effect
  chiS tfoX positive effect
  hapR dns negative effect

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62340.49 Da        Isoelectric Point: 4.6119

>NTDB_id=1175 A1552VC_RS12105 WP_000788426.1 2577285..2579000(-) (pilQ) [Vibrio cholerae strain A1552]
MKNGLKTYVAQTWLTLWVGLALCASSMVFSAESATANQLENIDFRVNKEKAAVLIVELASPSAVVDVQKVQEGLSIELLK
TDVADDKLYLLDVKDFSTPVESVEVFRKEPSTQLVVTVDGEFQHDYTLKGKYLEVVISKLKADEKPKPKSVLEKEGKLIS
INFQDIPVRNVLQLIADYNGFNLVVSDSVVGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILIAPKEELDLREKQAL
EKARLAEELGDLKSEIIKINFAKASDIAAMIGGEGNVNMLSERGSISIDERTNSLLIRELPDNIAVIREIIESLDIPVKQ
VQIEARIVTVKEGNLEELGVRWGVMSTNGSHSVGGSIESNLWQKGLLADDEFPVDEFLNVNLASTSANASSIAFQVAKLG
SGTLLDLELSALQNESKAEIISSPRLITTNKQPAYIEQGTEIPYLESSSSGASTVAFKKAVLSLKVTPQITPDNRLVLDL
SVTQDRRGETVKTGTGEAVSIDTQRIGTQVLVNNGETVVLGGIFQHSINNSVDKVPLLGDLPVLGALFRRTYEQMGKSEL
LIFVTPKVVIQ

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=1175 A1552VC_RS12105 WP_000788426.1 2577285..2579000(-) (pilQ) [Vibrio cholerae strain A1552]
ATGAAAAATGGATTGAAAACTTATGTCGCACAGACTTGGCTGACCTTGTGGGTTGGTTTGGCACTTTGTGCTTCTTCCAT
GGTGTTTAGTGCTGAATCCGCTACCGCCAACCAGTTGGAAAACATCGACTTTCGGGTCAACAAAGAGAAGGCGGCGGTCT
TAATTGTCGAATTGGCTTCGCCATCGGCGGTCGTGGATGTGCAAAAAGTCCAAGAAGGGTTGAGCATTGAACTCCTGAAA
ACCGATGTGGCGGATGACAAGCTGTATCTGCTTGATGTGAAAGATTTTTCGACCCCTGTTGAGAGCGTGGAAGTATTCCG
TAAAGAGCCAAGCACACAACTGGTTGTGACTGTCGATGGGGAGTTTCAGCACGATTATACCTTAAAAGGAAAATACCTTG
AGGTCGTGATCAGTAAACTCAAAGCCGATGAAAAGCCTAAGCCCAAGAGTGTCTTGGAAAAAGAAGGCAAGCTGATTTCA
ATTAACTTCCAAGATATTCCGGTGCGTAACGTACTGCAGCTGATTGCCGATTATAACGGTTTCAACTTAGTGGTTTCCGA
TTCGGTGGTGGGTAACCTGACTTTACGCTTGGATGGCGTGCCTTGGCAGCAAGTGTTGGACATTATTCTGCAAGTCAAAG
GGCTCGATAAACGGGTCGATGGTAATGTGATTTTGATTGCGCCGAAAGAGGAACTCGACTTGCGCGAGAAACAAGCGCTC
GAAAAAGCCCGTTTAGCAGAAGAGTTGGGCGATTTAAAATCAGAAATCATCAAGATTAACTTTGCCAAAGCGTCTGACAT
TGCGGCGATGATCGGCGGTGAAGGCAACGTTAACATGTTGTCTGAACGAGGTTCGATCAGCATCGACGAACGAACTAACT
CGCTATTGATCCGAGAGCTGCCCGATAATATTGCTGTGATCCGCGAGATCATTGAATCGCTGGATATTCCGGTCAAACAA
GTTCAAATCGAAGCGCGGATTGTCACCGTCAAAGAAGGTAACTTGGAGGAGCTGGGGGTACGCTGGGGCGTGATGTCGAC
CAATGGTAGCCACTCGGTCGGAGGAAGCATAGAGAGTAACTTATGGCAAAAAGGGTTATTGGCGGATGATGAATTTCCGG
TGGATGAATTCTTAAATGTGAATTTGGCTTCCACCTCTGCCAATGCGTCTTCGATTGCTTTCCAAGTCGCTAAATTGGGC
TCAGGTACGTTGTTGGATTTGGAGCTTTCAGCATTACAAAATGAATCGAAGGCCGAAATCATCTCTAGCCCTCGTTTGAT
TACCACTAATAAACAGCCTGCCTATATTGAGCAAGGTACTGAGATCCCTTATCTGGAATCGTCTTCCAGTGGTGCCTCTA
CGGTCGCGTTTAAAAAAGCGGTATTAAGCTTGAAGGTGACACCACAAATCACCCCAGATAACCGCTTGGTGCTCGACTTG
AGTGTGACCCAAGACCGACGCGGTGAAACGGTGAAAACCGGTACGGGTGAAGCGGTATCGATCGATACTCAGCGTATTGG
CACTCAAGTGTTGGTGAATAACGGAGAAACTGTGGTGTTGGGTGGCATTTTCCAACACAGCATCAATAATTCGGTAGATA
AGGTGCCACTGCTGGGGGACTTGCCTGTTTTGGGGGCGTTATTCAGACGAACCTATGAGCAAATGGGCAAAAGTGAACTG
CTGATTTTTGTCACGCCGAAAGTGGTCATTCAATAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(411-569)

Bacterial type II/III secretion system short domain (Secretin_N)

(255-320)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0K9UP29

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

100

100

1
  pilQ Vibrio campbellii strain DS40M4

78.545

96.322

0.757
  comE Haemophilus influenzae 86-028NP

40.625

100

0.409
  pilQ Legionella pneumophila strain Lp02

42.778

94.571

0.405
  pilQ Legionella pneumophila strain ERS1305867

42.778

94.571

0.405
  comQ Acinetobacter baylyi ADP1

39.336

100

0.394
  pilQ Acinetobacter baumannii D1279779

37.819

100

0.389
  pilQ Acinetobacter baumannii strain A118

37.819

100

0.389
  comE Haemophilus influenzae Rd KW20

41.748

92.584

0.387
  pilQ Pseudomonas aeruginosa PAK

39.344

96.147

0.378
  comE Glaesserella parasuis strain SC1401

37.923

98.571

0.374

Multiple sequence alignment    



References

[1] David W Adams et al. (2019) DNA-uptake pili of Vibrio cholerae are required for chitin colonization and capable of kin recognition via sequence-specific self-interaction. Nature Microbiology 4(9):1545-1557. [PMID: 31182799]
[2] Patrick Seitz et al. (2013) DNA-uptake machinery of naturally competent Vibrio cholerae. Proceedings of The National Academy of Sciences of The United States of America 110(44):17987-92. [PMID: 24127573]
[3] Mirella Lo Scrudato et al. (2012) The regulatory network of natural competence and transformation of Vibrio cholerae. PLoS Genetics 8(6):e1002778. [PMID: 22737089]
[4] Gaia Suckow et al. (2011) Quorum sensing contributes to natural transformation of Vibrio cholerae in a species-specific manner. Journal of Bacteriology 193(18):4914-24. [PMID: 21784943]
[5] Karin L Meibom et al. (2005) Chitin induces natural competence in Vibrio cholerae. Science (New York, N.Y.) 310(5755):1824-7. [PMID: 16357262]