Detailed information NTG0001347

Experimentally validated

Overview

• Name: comE
• Type: Machinery gene
• Locus tag: HI_0435
• Genome accession: L42023
• Coordinates: 456967..458304 (-)
• Length: 445 a.a.
• NCBI ID: AAC22094.1
• Uniprot ID: -
• Organism: Haemophilus influenzae Rd KW20
• Function: type IV pilus biogenesis and function DNA binding and uptake

Function

All genes in the comABCDE, comNOPQ, and pilABCD operons play essential roles in competence.

Genomic Context

Location: 451967..463304

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HI_0430	-	453698..454108 (-)	411	AAC22089.1	DNA-binding protein HU-alpha (hupA)	-
HI_0431	-	454110..454700 (-)	591	AAC22090.1	conserved hypothetical protein	-
HI_0432	-	454736..455530 (-)	795	AAC22091.1	conserved hypothetical protein	-
HI_0433	-	455597..456193 (-)	597	AAC22092.1	orfG protein	-
HI_0434	comF	456269..456958 (-)	690	AAC22093.1	competence protein F (comF)	Unclear
HI_0435	comE	456967..458304 (-)	1338	AAC22094.1	competence protein E (comE)	Machinery gene
HI_0436	comD	458314..458727 (-)	414	AAC22095.1	competence protein D (comD)	Machinery gene
HI_0437	comC	458724..459245 (-)	522	AAC22096.1	competence protein C (comC)	Machinery gene
HI_0438	comB	459242..459748 (-)	507	AAC22097.1	competence protein B (comB)	Machinery gene
HI_0439	comA	459749..460546 (-)	798	AAC22098.1	competence protein A (comA)	Machinery gene
HI_0440	-	460645..463239 (+)	2595	AAC22099.1	penicillin-binding protein 1A (ponA)	-

Sequence

Protein

• Length: 445 a.a.
• Molecular weight: 49208.48 Da
• Isoelectric Point: 7.4786

>NTDB_id=1347 HI_0435 AAC22094.1 456967..458304(-) (comE) [Haemophilus influenzae Rd KW20]
MKKYFLKCGYFLVCFCLPLIVFANPKTDNERFFIRLSQAPLAQTLEQLAFQQDVNLVIGDILENKISLKLNNIDMPRLLQ
IIAKSKHLTLNKDDGIYYLNGSQSGKGQVAGNLTTNEPHLVSHTVKLHFAKASELMKSLTTGSGSLLSPAGSITFDDRSN
LLVIQDEPRSVQNIKKLIAEMDKPIEQIAIEARIVTITDESLKELGVRWGIFNPTENARRVAGSLTGNSFENIADNLNVN
FATTTTPAGSIALQVAKINGRLLDLELSALERENNVEIIASPRLLTTNKKSASIKQGTEIPYIVSNTRNDTQSVEFREAV
LGLEVTPHISKDNNILLDLLVSQNSPGSRVAYGQNEVVSIDKQEINTQVFAKDGETIVLGGVFHDTITKSEDKVPLLGDI
PVIKRLFSKESERHQKRELVIFVTPHILKAGETLEALKQKSEGKK

Nucleotide

• Length: 1338 bp

>NTDB_id=1347 HI_0435 AAC22094.1 456967..458304(-) (comE) [Haemophilus influenzae Rd KW20]
ATGAAGAAATATTTTTTAAAGTGCGGTTATTTTTTAGTATGTTTTTGTTTGCCATTAATCGTTTTTGCTAATCCTAAAAC
AGATAACGAACGTTTTTTTATTCGTTTATCGCAAGCACCTTTAGCTCAAACACTGGAGCAATTAGCTTTTCAACAAGATG
TGAATTTAGTGATTGGAGATATATTGGAAAACAAGATCTCTTTGAAATTAAACAATATTGATATGCCACGTTTGCTACAA
ATAATCGCAAAAAGTAAGCATCTTACTTTGAATAAAGATGATGGGATTTATTATTTAAACGGCAGTCAATCTGGCAAAGG
TCAGGTTGCAGGAAATCTTACGACAAATGAACCGCACTTAGTGAGTCACACGGTAAAACTCCATTTTGCTAAAGCTTCTG
AATTAATGAAATCCTTAACAACAGGAAGTGGCTCTTTGCTTTCTCCCGCTGGGAGCATTACCTTTGATGATCGCAGTAAT
TTGCTGGTTATTCAGGATGAACCTCGTTCTGTGCAAAATATCAAAAAACTGATTGCTGAAATGGATAAGCCTATTGAACA
GATCGCTATTGAAGCGCGAATTGTGACAATTACGGATGAGAGTTTGAAAGAACTTGGCGTTCGGTGGGGGATTTTTAATC
CAACTGAAAATGCAAGACGAGTTGCGGGCAGCCTTACAGGCAATAGCTTTGAAAATATTGCGGATAATCTTAATGTAAAT
TTTGCGACAACGACGACACCTGCTGGCTCTATAGCATTACAAGTCGCCAAAATTAATGGGCGATTGCTTGATTTAGAATT
GAGTGCGTTGGAGCGTGAAAATAATGTAGAAATTATTGCAAGCCCTCGCTTACTCACTACCAATAAGAAAAGTGCGAGCA
TTAAACAGGGGACAGAAATTCCTTACATCGTGAGTAATACTCGTAACGATACGCAATCTGTGGAATTTCGTGAGGCGGTG
CTTGGTTTGGAAGTGACGCCACATATTTCTAAAGATAACAATATCTTACTTGATTTATTGGTAAGTCAAAATTCCCCTGG
TTCTCGTGTCGCTTATGGACAAAATGAGGTGGTTTCTATTGATAAACAAGAAATTAATACTCAGGTTTTTGCCAAAGATG
GGGAAACCATTGTGCTTGGCGGCGTATTTCACGATACAATCACGAAAAGCGAAGATAAAGTGCCATTGCTTGGCGATATA
CCCGTTATTAAACGATTATTTAGCAAAGAAAGTGAACGACATCAAAAACGTGAGCTAGTGATTTTCGTCACGCCACATAT
TTTAAAAGCAGGAGAAACGTTAGAGGCGTTGAAACAAAAAAGTGAGGGTAAAAAATAA

Domains

Predicted by InterproScan.

• Bacterial type II and III secretion system protein (Secretin) (269-429)

• Bacterial type II/III secretion system short domain (Secretin_N) (123-187)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comE	Haemophilus influenzae 86-028NP	97.528	100	0.975
comE	Glaesserella parasuis strain SC1401	52.941	100	0.536
pilQ	Vibrio campbellii strain DS40M4	42.482	94.157	0.4
pilQ	Vibrio cholerae strain A1552	41.748	92.584	0.387
pilQ	Vibrio cholerae O1 biovar El Tor strain E7946	41.748	92.584	0.387

References

• [1]: Sunita Sinha et al. (2012) Seventeen Sxy-dependent cyclic AMP receptor protein site-regulated genes are needed for natural transformation in Haemophilus influenzae. Journal of Bacteriology 194(19):5245-54. [PMID: 22821979]
• [2]: Brian A Dougherty et al. (1999) Identification of Haemophilus influenzae Rd transformation genes using cassette mutagenesis. Microbiology (Reading, England) 145 ( Pt 2):401-409. [PMID: 10075422]