Detailed information    

experimental Experimentally validated

Overview

Name   comA   Type   Machinery gene
Locus tag   HI_0439 Genome accession   L42023
Coordinates   459749..460546 (-) Length   265 a.a.
NCBI ID   AAC22098.1    Uniprot ID   -
Organism   Haemophilus influenzae Rd KW20     
Function   type IV pilus biogenesis and function   
DNA binding and uptake

Function

All genes in the comABCDE, comNOPQ, and pilABCD operons play essential roles in competence.


Genomic Context

Location: 454749..465546
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HI_0433 - 455597..456193 (-) 597 AAC22092.1 orfG protein -
  HI_0434 comF 456269..456958 (-) 690 AAC22093.1 competence protein F (comF) Unclear
  HI_0435 comE 456967..458304 (-) 1338 AAC22094.1 competence protein E (comE) Machinery gene
  HI_0436 comD 458314..458727 (-) 414 AAC22095.1 competence protein D (comD) Machinery gene
  HI_0437 comC 458724..459245 (-) 522 AAC22096.1 competence protein C (comC) Machinery gene
  HI_0438 comB 459242..459748 (-) 507 AAC22097.1 competence protein B (comB) Machinery gene
  HI_0439 comA 459749..460546 (-) 798 AAC22098.1 competence protein A (comA) Machinery gene
  HI_0440 - 460645..463239 (+) 2595 AAC22099.1 penicillin-binding protein 1A (ponA) -
  HI_0441 - 463327..464172 (+) 846 AAC22100.1 orfJ protein -
  HI_0442 - 464289..464654 (+) 366 AAC22101.1 conserved hypothetical protein -
  HI_0443 - 464716..465318 (+) 603 AAC22102.1 recombination protein (recR) -

Sequence

Protein


Download         Length: 265 a.a.        Molecular weight: 31541.85 Da        Isoelectric Point: 5.9495

>NTDB_id=1343 HI_0439 AAC22098.1 459749..460546(-) (comA) [Haemophilus influenzae Rd KW20]
MQFSLKNYRTLQIGIHRKQSYFDFVWFDDLEQPQSYQIFVNDRYFKNRFLQQLKTQYQGKTFPLQFVASIPAHLTWSKVL
MLPQVLNAQECHQQCKFVIEKELPIFLEELWFDYRSTPLKQGFRLEVTAIRKSSAQTYLQDFQPFNINILDVASNAVLRA
FQYLLNEQVRSENTLFLFQEDDYCLAICERSQQSQILQSHENLTALYEQFTERFEGQLEQVFVYQIPSSHTPLPENWQRV
ETELPFIALGNALWQKDLHQQKVGG

Nucleotide


Download         Length: 798 bp        

>NTDB_id=1343 HI_0439 AAC22098.1 459749..460546(-) (comA) [Haemophilus influenzae Rd KW20]
ATGCAATTCTCCCTGAAAAATTACCGCACTTTACAAATCGGCATTCATCGTAAGCAGAGTTATTTTGATTTTGTGTGGTT
TGATGATCTCGAACAGCCACAAAGTTATCAAATCTTTGTTAATGATCGTTATTTTAAAAATCGTTTTTTACAACAGCTAA
AAACACAATATCAAGGGAAAACCTTTCCTTTGCAGTTTGTAGCAAGCATTCCCGCCCACTTAACTTGGTCGAAAGTATTA
ATGTTGCCACAAGTGTTAAATGCGCAAGAATGTCATCAACAATGTAAATTTGTGATTGAAAAAGAGCTGCCTATTTTTTT
AGAAGAATTGTGGTTTGATTATCGTTCTACCCCGTTAAAGCAAGGTTTTCGATTAGAGGTTACTGCAATTCGTAAAAGTA
GCGCTCAAACTTATTTGCAAGATTTTCAGCCATTTAATATTAATATATTGGATGTTGCGTCAAATGCTGTTTTGCGTGCA
TTTCAATATCTGTTGAATGAACAAGTGCGGTCAGAAAATACCTTATTTTTATTTCAAGAAGATGACTATTGCTTGGCGAT
TTGTGAAAGATCTCAGCAATCACAAATTTTACAATCTCACGAAAATTTGACCGCACTTTATGAACAATTTACCGAACGTT
TTGAAGGACAACTTGAACAAGTTTTTGTTTATCAAATTCCCTCAAGTCATACACCATTACCCGAAAACTGGCAGCGAGTA
GAAACAGAACTCCCTTTTATTGCGCTGGGCAACGCGCTATGGCAAAAAGATTTACATCAACAAAAAGTGGGTGGTTAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comA Haemophilus influenzae 86-028NP

96.226

100

0.962

Multiple sequence alignment    



References

[1] Sunita Sinha et al. (2012) Seventeen Sxy-dependent cyclic AMP receptor protein site-regulated genes are needed for natural transformation in Haemophilus influenzae. Journal of Bacteriology 194(19):5245-54. [PMID: 22821979]
[2] Brian A Dougherty et al. (1999) Identification of Haemophilus influenzae Rd transformation genes using cassette mutagenesis. Microbiology (Reading, England) 145 ( Pt 2):401-409. [PMID: 10075422]