Detailed information    

experimental Experimentally validated

Overview

Name   comB   Type   Machinery gene
Locus tag   HI_0438 Genome accession   L42023
Coordinates   459242..459748 (-) Length   168 a.a.
NCBI ID   AAC22097.1    Uniprot ID   -
Organism   Haemophilus influenzae Rd KW20     
Function   type IV pilus biogenesis and function   
DNA binding and uptake

Function

All genes in the comABCDE, comNOPQ, and pilABCD operons play essential roles in competence.


Genomic Context

Location: 454242..464748
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HI_0432 - 454736..455530 (-) 795 AAC22091.1 conserved hypothetical protein -
  HI_0433 - 455597..456193 (-) 597 AAC22092.1 orfG protein -
  HI_0434 comF 456269..456958 (-) 690 AAC22093.1 competence protein F (comF) Unclear
  HI_0435 comE 456967..458304 (-) 1338 AAC22094.1 competence protein E (comE) Machinery gene
  HI_0436 comD 458314..458727 (-) 414 AAC22095.1 competence protein D (comD) Machinery gene
  HI_0437 comC 458724..459245 (-) 522 AAC22096.1 competence protein C (comC) Machinery gene
  HI_0438 comB 459242..459748 (-) 507 AAC22097.1 competence protein B (comB) Machinery gene
  HI_0439 comA 459749..460546 (-) 798 AAC22098.1 competence protein A (comA) Machinery gene
  HI_0440 - 460645..463239 (+) 2595 AAC22099.1 penicillin-binding protein 1A (ponA) -
  HI_0441 - 463327..464172 (+) 846 AAC22100.1 orfJ protein -
  HI_0442 - 464289..464654 (+) 366 AAC22101.1 conserved hypothetical protein -

Sequence

Protein


Download         Length: 168 a.a.        Molecular weight: 19750.69 Da        Isoelectric Point: 8.5473

>NTDB_id=1344 HI_0438 AAC22097.1 459242..459748(-) (comB) [Haemophilus influenzae Rd KW20]
MSMNLLPWRTYQHQKRLRRLAFYIALFILLAINLMLAFSNLIEQQKQNLQAQQKSFEQLNQQLHKTTMQIDQLRIAVKVG
EVLTSIPNEQVKKSLQQLSELPFQQGELNKFKQDANNLSLEGNAQDQTEFELIHQFLKKHFPNVKLSQVQPEQDTLFFHF
DVEQGAEK

Nucleotide


Download         Length: 507 bp        

>NTDB_id=1344 HI_0438 AAC22097.1 459242..459748(-) (comB) [Haemophilus influenzae Rd KW20]
ATGTCGATGAATTTATTGCCTTGGCGTACTTATCAACATCAAAAGCGTTTACGTCGTTTAGCTTTTTATATCGCTTTATT
TATCTTGCTTGCTATTAATTTAATGTTGGCTTTTAGCAATTTGATTGAACAACAGAAACAAAATTTGCAGGCACAGCAAA
AGTCGTTTGAACAACTTAATCAACAGCTTCATAAAACTACCATGCAAATTGATCAGTTACGCATTGCGGTGAAAGTTGGT
GAAGTTTTGACATCTATTCCCAACGAGCAAGTAAAAAAGAGTTTACAACAGCTAAGTGAATTACCTTTTCAACAAGGAGA
ACTGAATAAATTTAAACAAGATGCCAATAACTTAAGCTTGGAAGGTAACGCGCAAGATCAAACAGAATTTGAACTGATTC
ATCAATTTTTAAAGAAACATTTTCCCAATGTGAAATTAAGTCAGGTTCAACCTGAACAAGATACATTGTTTTTTCACTTT
GATGTGGAACAAGGGGCGGAAAAATGA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comB Haemophilus influenzae 86-028NP

98.81

100

0.988

Multiple sequence alignment    



References

[1] Sunita Sinha et al. (2012) Seventeen Sxy-dependent cyclic AMP receptor protein site-regulated genes are needed for natural transformation in Haemophilus influenzae. Journal of Bacteriology 194(19):5245-54. [PMID: 22821979]
[2] Brian A Dougherty et al. (1999) Identification of Haemophilus influenzae Rd transformation genes using cassette mutagenesis. Microbiology (Reading, England) 145 ( Pt 2):401-409. [PMID: 10075422]