Detailed information    

experimental Experimentally validated

Overview

Name   comD   Type   Machinery gene
Locus tag   HI_0436 Genome accession   L42023
Coordinates   458314..458727 (-) Length   137 a.a.
NCBI ID   AAC22095.1    Uniprot ID   -
Organism   Haemophilus influenzae Rd KW20     
Function   type IV pilus biogenesis and function   
DNA binding and uptake

Function

All genes in the comABCDE, comNOPQ, and pilABCD operons play essential roles in competence.


Genomic Context

Location: 453314..463727
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HI_0430 - 453698..454108 (-) 411 AAC22089.1 DNA-binding protein HU-alpha (hupA) -
  HI_0431 - 454110..454700 (-) 591 AAC22090.1 conserved hypothetical protein -
  HI_0432 - 454736..455530 (-) 795 AAC22091.1 conserved hypothetical protein -
  HI_0433 - 455597..456193 (-) 597 AAC22092.1 orfG protein -
  HI_0434 comF 456269..456958 (-) 690 AAC22093.1 competence protein F (comF) Unclear
  HI_0435 comE 456967..458304 (-) 1338 AAC22094.1 competence protein E (comE) Machinery gene
  HI_0436 comD 458314..458727 (-) 414 AAC22095.1 competence protein D (comD) Machinery gene
  HI_0437 comC 458724..459245 (-) 522 AAC22096.1 competence protein C (comC) Machinery gene
  HI_0438 comB 459242..459748 (-) 507 AAC22097.1 competence protein B (comB) Machinery gene
  HI_0439 comA 459749..460546 (-) 798 AAC22098.1 competence protein A (comA) Machinery gene
  HI_0440 - 460645..463239 (+) 2595 AAC22099.1 penicillin-binding protein 1A (ponA) -

Sequence

Protein


Download         Length: 137 a.a.        Molecular weight: 15681.82 Da        Isoelectric Point: 4.5625

>NTDB_id=1346 HI_0436 AAC22095.1 458314..458727(-) (comD) [Haemophilus influenzae Rd KW20]
MKHWFFLIILFFMNCSWGQDPFDKTQRNRSQFDNAQTVMEQTEIISSDVPNNLCGADENRQAAEIPLNALKLVGVVISKD
KAFALLQDQGLQVYSVLEGVDVAQEGYIVEKINQNNVQFMRKLGEQCDSSEWKKLSF

Nucleotide


Download         Length: 414 bp        

>NTDB_id=1346 HI_0436 AAC22095.1 458314..458727(-) (comD) [Haemophilus influenzae Rd KW20]
ATGAAACATTGGTTTTTCCTGATTATATTATTTTTTATGAATTGCAGTTGGGGACAAGATCCTTTCGATAAAACACAGCG
TAACCGTTCTCAGTTTGATAACGCACAAACAGTAATGGAGCAAACAGAAATAATTTCCTCAGATGTGCCTAATAATCTAT
GCGGAGCGGATGAAAATCGCCAAGCGGCTGAAATTCCTTTGAACGCTTTAAAATTGGTGGGGGTAGTGATTTCTAAAGAT
AAAGCCTTTGCCTTGTTGCAAGATCAAGGTTTGCAAGTTTACAGCGTTTTAGAGGGCGTTGATGTGGCTCAAGAGGGCTA
TATTGTAGAAAAAATCAACCAAAACAATGTTCAATTTATGCGTAAGCTAGGAGAGCAATGTGATAGTAGTGAATGGAAAA
AATTAAGTTTTTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilP (PilP)

(26-123)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comD Haemophilus influenzae 86-028NP

99.27

100

0.993

Multiple sequence alignment    



References

[1] Sunita Sinha et al. (2012) Seventeen Sxy-dependent cyclic AMP receptor protein site-regulated genes are needed for natural transformation in Haemophilus influenzae. Journal of Bacteriology 194(19):5245-54. [PMID: 22821979]
[2] Brian A Dougherty et al. (1999) Identification of Haemophilus influenzae Rd transformation genes using cassette mutagenesis. Microbiology (Reading, England) 145 ( Pt 2):401-409. [PMID: 10075422]