Detailed information    

experimental Experimentally validated

Overview

Name   comD   Type   Machinery gene
Locus tag   NTHI_RS02650 Genome accession   NC_007146
Coordinates   524935..525348 (-) Length   137 a.a.
NCBI ID   WP_011272047.1    Uniprot ID   Q4QNA9
Organism   Haemophilus influenzae 86-028NP     
Function   type IV pilus biogenesis and function   
DNA binding and uptake

Function

The pilABCD and comABCDEF gene clusters are operons and that the product of each gene is essential for normal pilus function. Mutants with nonpolar deletions in each of the 10 pil and com genes had an adherence defect when primary human airway cells were used as the target. These mutants were also diminished in their ability to form a biofilm in vitro and, additionally, were deficient in natural transformation.


Genomic Context

Location: 519935..530348
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NTHI_RS02620 (NTHI0554) - 520318..520590 (-) 273 WP_005630954.1 HU family DNA-binding protein -
  NTHI_RS02625 (NTHI0555) - 520730..521320 (-) 591 WP_005686892.1 YjaG family protein -
  NTHI_RS02630 (NTHI0557) nudC 521356..522150 (-) 795 WP_005686893.1 NAD(+) diphosphatase -
  NTHI_RS02635 (NTHI0558) nfuA 522217..522813 (-) 597 WP_005649300.1 Fe-S biogenesis protein NfuA -
  NTHI_RS02640 (NTHI0559) comF 522889..523575 (-) 687 WP_011272045.1 ComF family protein Unclear
  NTHI_RS02645 (NTHI0560) comE 523588..524925 (-) 1338 WP_011272046.1 type IV pilus secretin PilQ Machinery gene
  NTHI_RS02650 (NTHI0562) comD 524935..525348 (-) 414 WP_011272047.1 pilus assembly protein PilP Machinery gene
  NTHI_RS02655 (NTHI0563) comC 525345..525866 (-) 522 WP_011272048.1 competence protein ComC Machinery gene
  NTHI_RS02660 (NTHI0564) comB 525863..526369 (-) 507 WP_011272049.1 competence protein ComB Machinery gene
  NTHI_RS02665 (NTHI0566) comA 526370..527167 (-) 798 WP_011272050.1 pilus assembly protein PilM Machinery gene
  NTHI_RS02670 (NTHI0567) - 527266..529860 (+) 2595 WP_011272051.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 137 a.a.        Molecular weight: 15695.85 Da        Isoelectric Point: 4.5625

>NTDB_id=1372 NTHI_RS02650 WP_011272047.1 524935..525348(-) (comD) [Haemophilus influenzae 86-028NP]
MKHWFFLIILFFMNCSWGQDPFDKTQRNRSQFDNAQTVMEQTEIISSDVPNNLCGADENRQAAEIPLNALKLVGVVISKD
KAFALLQDQGLQIYSVLEGVDVAQEGYIVEKINQNNVQFMRKLGEQCDSSEWKKLSF

Nucleotide


Download         Length: 414 bp        

>NTDB_id=1372 NTHI_RS02650 WP_011272047.1 524935..525348(-) (comD) [Haemophilus influenzae 86-028NP]
ATGAAACATTGGTTTTTCCTGATTATATTATTTTTTATGAATTGCAGTTGGGGACAAGATCCTTTCGATAAAACACAGCG
TAACCGTTCTCAGTTTGATAACGCACAAACAGTAATGGAGCAGACAGAAATAATTTCCTCAGATGTACCTAATAATCTAT
GCGGAGCGGATGAAAATCGCCAAGCGGCTGAAATTCCTTTGAACGCTTTAAAATTGGTGGGCGTAGTGATTTCTAAAGAT
AAAGCCTTTGCCTTGTTGCAAGATCAAGGTTTGCAAATTTACAGCGTTTTAGAGGGCGTTGATGTGGCTCAAGAGGGCTA
TATTGTAGAAAAAATCAACCAAAACAATGTTCAATTTATGCGTAAGCTCGGAGAGCAATGTGATAGTAGTGAATGGAAAA
AATTAAGTTTTTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilP (PilP)

(26-123)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q4QNA9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comD Haemophilus influenzae Rd KW20

99.27

100

0.993

Multiple sequence alignment    



References

[1] Michael D Carruthers et al. (2012) Biological roles of nontypeable Haemophilus influenzae type IV pilus proteins encoded by the pil and com operons. Journal of Bacteriology 194(8):1927-33. [PMID: 22328674]