Detailed information    

experimental Experimentally validated

Overview

Name   comC   Type   Machinery gene
Locus tag   NTHI_RS02655 Genome accession   NC_007146
Coordinates   525345..525866 (-) Length   173 a.a.
NCBI ID   WP_011272048.1    Uniprot ID   Q4QNA8
Organism   Haemophilus influenzae 86-028NP     
Function   type IV pilus biogenesis and function   
DNA binding and uptake

Function

The pilABCD and comABCDEF gene clusters are operons and that the product of each gene is essential for normal pilus function. Mutants with nonpolar deletions in each of the 10 pil and com genes had an adherence defect when primary human airway cells were used as the target. These mutants were also diminished in their ability to form a biofilm in vitro and, additionally, were deficient in natural transformation.


Genomic Context

Location: 520345..530866
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NTHI_RS02625 (NTHI0555) - 520730..521320 (-) 591 WP_005686892.1 YjaG family protein -
  NTHI_RS02630 (NTHI0557) nudC 521356..522150 (-) 795 WP_005686893.1 NAD(+) diphosphatase -
  NTHI_RS02635 (NTHI0558) nfuA 522217..522813 (-) 597 WP_005649300.1 Fe-S biogenesis protein NfuA -
  NTHI_RS02640 (NTHI0559) comF 522889..523575 (-) 687 WP_011272045.1 ComF family protein Unclear
  NTHI_RS02645 (NTHI0560) comE 523588..524925 (-) 1338 WP_011272046.1 type IV pilus secretin PilQ Machinery gene
  NTHI_RS02650 (NTHI0562) comD 524935..525348 (-) 414 WP_011272047.1 pilus assembly protein PilP Machinery gene
  NTHI_RS02655 (NTHI0563) comC 525345..525866 (-) 522 WP_011272048.1 competence protein ComC Machinery gene
  NTHI_RS02660 (NTHI0564) comB 525863..526369 (-) 507 WP_011272049.1 competence protein ComB Machinery gene
  NTHI_RS02665 (NTHI0566) comA 526370..527167 (-) 798 WP_011272050.1 pilus assembly protein PilM Machinery gene
  NTHI_RS02670 (NTHI0567) - 527266..529860 (+) 2595 WP_011272051.1 penicillin-binding protein 1A -
  NTHI_RS02675 (NTHI0568) - 529933..530778 (+) 846 WP_011272052.1 23S rRNA (adenine(2030)-N(6))-methyltransferase RlmJ -

Sequence

Protein


Download         Length: 173 a.a.        Molecular weight: 19994.18 Da        Isoelectric Point: 10.0952

>NTDB_id=1371 NTHI_RS02655 WP_011272048.1 525345..525866(-) (comC) [Haemophilus influenzae 86-028NP]
MKAFFNDPFTPFGKWLSQPFYVHGLTFLLLLSAVIFRPVLDYIEGSSRFHEIENELAVKRSELLHQQKILTSLQQQSESR
KLSPELAAQIIPLNKQIQRLAARNGLSQHLRWEMGQKPILHLQLTGHFEKTKTFLTALLANSSQLSVSRLQFIKPEDNPL
QTEIIFQLDKETK

Nucleotide


Download         Length: 522 bp        

>NTDB_id=1371 NTHI_RS02655 WP_011272048.1 525345..525866(-) (comC) [Haemophilus influenzae 86-028NP]
ATGAAAGCTTTTTTTAACGATCCTTTTACTCCTTTTGGAAAATGGCTAAGTCAGCCTTTTTATGTGCACGGTTTAACCTT
TTTATTGCTATTAAGTGCGGTGATTTTTCGCCCCGTTTTAGATTATATCGAGGGGAGTTCACGTTTCCATGAAATTGAAA
ATGAGTTAGCGGTGAAACGTTCAGAATTGTTGCATCAACAGAAAATTTTAACTTCTTTACAGCAGCAGTCGGAAAGTCGA
AAACTTTCTCCAGAACTGGCTGCACAAATTATTCCTTTGAATAAACAAATTCAACGTTTAGCTGCGCGTAACGGTTTATC
TCAGCATTTACGTTGGGAAATGGGGCAAAAGCCTATTTTGCATTTACAGCTTACAGGTCATTTTGAGAAAACGAAGACAT
TTTTAACCGCACTTTTGGCTAATTCGTCACAGCTTTCAGTGAGTCGCTTGCAGTTTATCAAACCCGAAGACAACCCATTG
CAAACCGAGATCATTTTTCAGCTAGATAAGGAAACAAAATGA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q4QNA8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comC Haemophilus influenzae Rd KW20

98.266

100

0.983

Multiple sequence alignment    



References

[1] Michael D Carruthers et al. (2012) Biological roles of nontypeable Haemophilus influenzae type IV pilus proteins encoded by the pil and com operons. Journal of Bacteriology 194(8):1927-33. [PMID: 22328674]