Detailed information NTG0001374

Experimentally validated

Overview

• Name: comF
• Type: Unclear
• Locus tag: NTHI_RS02640
• Genome accession: NC_007146
• Coordinates: 522889..523575 (-)
• Length: 228 a.a.
• NCBI ID: WP_011272045.1
• Uniprot ID: Q4QNB1
• Organism: Haemophilus influenzae 86-028NP
• Function: require for natural transformation Unclear

Function

The pilABCD and comABCDEF gene clusters are operons and that the product of each gene is essential for normal pilus function. Mutants with nonpolar deletions in each of the 10 pil and com genes had an adherence defect when primary human airway cells were used as the target. These mutants were also diminished in their ability to form a biofilm in vitro and, additionally, were deficient in natural transformation.

Genomic Context

Location: 517889..528575

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NTHI_RS02615 (NTHI0553)	glmS	518373..520205 (-)	1833	WP_005686891.1	glutamine--fructose-6-phosphate transaminase (isomerizing)	-
NTHI_RS02620 (NTHI0554)	-	520318..520590 (-)	273	WP_005630954.1	HU family DNA-binding protein	-
NTHI_RS02625 (NTHI0555)	-	520730..521320 (-)	591	WP_005686892.1	YjaG family protein	-
NTHI_RS02630 (NTHI0557)	nudC	521356..522150 (-)	795	WP_005686893.1	NAD(+) diphosphatase	-
NTHI_RS02635 (NTHI0558)	nfuA	522217..522813 (-)	597	WP_005649300.1	Fe-S biogenesis protein NfuA	-
NTHI_RS02640 (NTHI0559)	comF	522889..523575 (-)	687	WP_011272045.1	ComF family protein	Unclear
NTHI_RS02645 (NTHI0560)	comE	523588..524925 (-)	1338	WP_011272046.1	type IV pilus secretin PilQ	Machinery gene
NTHI_RS02650 (NTHI0562)	comD	524935..525348 (-)	414	WP_011272047.1	pilus assembly protein PilP	Machinery gene
NTHI_RS02655 (NTHI0563)	comC	525345..525866 (-)	522	WP_011272048.1	competence protein ComC	Machinery gene
NTHI_RS02660 (NTHI0564)	comB	525863..526369 (-)	507	WP_011272049.1	competence protein ComB	Machinery gene
NTHI_RS02665 (NTHI0566)	comA	526370..527167 (-)	798	WP_011272050.1	pilus assembly protein PilM	Machinery gene

Sequence

Protein

• Length: 228 a.a.
• Molecular weight: 26682.07 Da
• Isoelectric Point: 10.3735

>NTDB_id=1374 NTHI_RS02640 WP_011272045.1 522889..523575(-) (comF) [Haemophilus influenzae 86-028NP]
MNFFNFRCIHCRGNLHIAKNGLCSGCQKQIKSFPYCGHCGAELQYYAQHCGNCLKQEPSWDKMVIIGHYIEPLSILIHRF
KFQNQFWIDRTLARLLYLAVRDAKRTHQLKLPEAIIPVPLYHFRQWRRGYNQADLLSRQLSRWLDIPNLSNIVKRVKHTY
TQRGLSAKDRRQNLKNAFSLVVSKNEFPYRRVALVDDVITTGSTLNEISKLLRKLGVEEIQVWGLARA

Nucleotide

• Length: 687 bp

>NTDB_id=1374 NTHI_RS02640 WP_011272045.1 522889..523575(-) (comF) [Haemophilus influenzae 86-028NP]
ATGAATTTTTTTAATTTTCGCTGTATCCACTGTCGTGGCAATCTTCATATTGCAAAAAATGGGCTATGTTCAGGTTGCCA
AAAACAAATTAAATCTTTTCCTTATTGCGGTCATTGTGGTGCGGAATTGCAATATTATGCGCAGCATTGTGGTAATTGTC
TTAAACAAGAACCAAGTTGGGATAAGATGGTCATTATTGGGCATTATATTGAACCTCTTTCGATATTGATTCACCGTTTT
AAATTTCAAAATCAATTTTGGATTGACCGCACTTTAGCTCGGCTTTTATATCTTGCGGTGCGTGATGCTAAACGAACGCA
TCAACTTAAATTGCCAGAAGCAATCATTCCAGTGCCTTTATATCATTTTCGTCAGTGGCGACGGGGTTATAATCAGGCAG
ATTTATTATCTCGGCAATTAAGTCGCTGGCTGGATATTCCTAATTTGAGCAATATCGTAAAGCGTGTGAAACACACCTAT
ACTCAACGTGGTTTGAGTGCAAAAGATCGTCGTCAGAATTTAAAAAATGCCTTTTCTCTTGTTGTTTCGAAAAATGAATT
TCCTTATCGCCGTGTTGCGTTGGTGGATGATGTGATTACTACTGGTTCTACACTCAATGAAATCTCAAAATTGTTGCGAA
AATTAGGTGTGGAGGAGATTCAAGTGTGGGGGCTGGCACGAGCTTAA

Domains

Predicted by InterproScan.

• Phosphoribosyl transferase domain (Pribosyltran) (173-221)

3D structure

• AlphaFold DB: Q4QNB1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comF	Haemophilus influenzae Rd KW20	97.807	100	0.978
comFC	Legionella pneumophila strain ERS1305867	37.113	98.98	0.367

References

• [1]: Michael D Carruthers et al. (2012) Biological roles of nontypeable Haemophilus influenzae type IV pilus proteins encoded by the pil and com operons. Journal of Bacteriology 194(8):1927-33. [PMID: 22328674]