Detailed information NTG0001247

Experimentally validated

Overview

• Name: comFC
• Type: Machinery gene
• Locus tag: GCO85_RS11680
• Genome accession: NZ_CP048618
• Coordinates: 2625726..2626430 (+)
• Length: 234 a.a.
• NCBI ID: WP_015961431.1
• Uniprot ID: -
• Organism: Legionella pneumophila strain ERS1305867
• Function: ssDNA transport into the cell DNA binding and uptake

Function

Mutation of this gene caused a decrease in transformation.

Genomic Context

Location: 2620726..2631430

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GCO85_RS11655 (GCO85_11665)	-	2621957..2622835 (+)	879	WP_027225912.1	lpg2327 family Dot/Icm T4SS effector	-
GCO85_RS11660 (GCO85_11670)	lem22	2623100..2623483 (+)	384	WP_014842288.1	Dot/Icm T4SS effector Lem22	-
GCO85_RS11665 (GCO85_11675)	-	2623677..2623907 (+)	231	WP_011947173.1	exodeoxyribonuclease VII small subunit	-
GCO85_RS11670 (GCO85_11680)	-	2623888..2624784 (+)	897	WP_011947174.1	polyprenyl synthetase family protein	-
GCO85_RS11675 (GCO85_11685)	bioC	2624828..2625682 (-)	855	WP_015961430.1	malonyl-ACP O-methyltransferase BioC	-
-	comFC	2625726..2626430 (+)	705	-	-	Machinery gene
GCO85_RS11680 (GCO85_11690)	-	2625840..2626430 (+)	591	WP_014844481.1	ComF family protein	-
GCO85_RS11685 (GCO85_11695)	-	2626459..2627532 (-)	1074	WP_061636231.1	site-2 protease family protein	-
GCO85_RS11690 (GCO85_11700)	-	2627589..2627867 (-)	279	WP_010948040.1	hypothetical protein	-
GCO85_RS11695 (GCO85_11705)	-	2627906..2628061 (+)	156	WP_162096598.1	hypothetical protein	-
GCO85_RS11700 (GCO85_11710)	hemA	2628185..2629459 (+)	1275	WP_015444133.1	glutamyl-tRNA reductase	-
GCO85_RS11705 (GCO85_11715)	prfA	2629443..2630531 (+)	1089	WP_042755102.1	peptide chain release factor 1	-
GCO85_RS11710 (GCO85_11720)	prmC	2630524..2631387 (+)	864	WP_027225915.1	peptide chain release factor N(5)-glutamine methyltransferase	-

Sequence

Protein

• Length: 234 a.a.
• Molecular weight: 22371.12 Da
• Isoelectric Point: 9.4846

>NTDB_id=1247 GCO85_RS11680 WP_015961431.1 2625726..2626430(+) (comFC) [Legionella pneumophila strain ERS1305867]
MKQLGSCCQYCAYPLSDDTYLVCGQCVRKRPSFDSAYIAYRFEEPLRSLIHQFKYHNGLYLASFLKQLLLNALPKSALKP
DCLIPVPMHPKRLKRRGFNQAAVLTRLLARQLNIPYDLYYCQKIINTASQANLDGEQRRKNLRHAFYVPPVTYEHVMIVD
DLLTTGSTANEIAHTLKNAGVKRVDICCCARAVTKN

Nucleotide

• Length: 705 bp

>NTDB_id=1247 GCO85_RS11680 WP_015961431.1 2625726..2626430(+) (comFC) [Legionella pneumophila strain ERS1305867]
GTGCTCCAAAAATTCTTAAGTATAACACAGAACTTACGTTTACCTTCAATATGCACTCTTTGCAACCAGTTCCACAAAAG
CCAACTTGCAGTTTGCTCTAATTGCATGGAGTTTATGAAACAACTTGGATCTTGCTGTCAGTACTGTGCCTACCCCCTTT
CTGATGATACGTATCTGGTTTGTGGTCAATGCGTTAGAAAAAGGCCCTCCTTTGATAGTGCTTATATAGCGTATCGTTTT
GAAGAGCCTCTGCGTAGTTTAATACATCAGTTTAAATACCATAATGGCCTTTATCTGGCCTCTTTTCTAAAGCAATTACT
TCTTAATGCCTTACCCAAAAGCGCATTGAAACCAGATTGTCTAATTCCCGTTCCCATGCATCCCAAACGATTAAAACGAA
GGGGATTTAACCAGGCTGCTGTATTAACCAGATTATTAGCTCGTCAATTAAACATCCCTTATGATTTATATTATTGCCAA
AAAATAATCAATACCGCTTCACAAGCCAACCTTGATGGAGAGCAACGAAGAAAAAATTTACGTCATGCTTTTTATGTACC
ACCTGTAACTTATGAACACGTCATGATTGTTGATGACTTATTAACCACAGGCAGCACAGCCAATGAAATAGCACATACAT
TAAAAAATGCCGGGGTTAAGAGAGTAGATATATGTTGTTGTGCGCGGGCTGTAACTAAGAATTAA

Domains

Predicted by InterproScan.

• Phosphoribosyl transferase domain (Pribosyltran) (102-191)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFC	Legionella pneumophila str. Paris	100	100	1
comF	Haemophilus influenzae 86-028NP	37.113	98.98	0.367
comF	Haemophilus influenzae Rd KW20	36.598	98.98	0.362

References

• [1]: Léo Hardy et al. (2021) Transposon Insertion Sequencing in a Clinical Isolate of Legionella pneumophila Identifies Essential Genes and Determinants of Natural Transformation. Journal of Bacteriology 203(3):e00548-20. [PMID: 33168636]