Detailed information    

experimental Experimentally validated

Overview

Name   comFC   Type   Machinery gene
Locus tag   LPP_RS11540 Genome accession   NC_006368
Coordinates   2621502..2622206 (+) Length   234 a.a.
NCBI ID   WP_015961431.1    Uniprot ID   -
Organism   Legionella pneumophila str. Paris     
Function   ssDNA transport into the cell   
DNA binding and uptake

Function

Mutation of this gene caused a decrease in transformation.


Genomic Context

Location: 2616502..2627206
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LPP_RS11515 (lpp2275) - 2617733..2618611 (+) 879 WP_015961428.1 lpg2327 family Dot/Icm T4SS effector -
  LPP_RS11520 (lpp2276) lem22 2618876..2619259 (+) 384 WP_014842288.1 Dot/Icm T4SS effector Lem22 -
  LPP_RS11525 (lpp2277) - 2619453..2619683 (+) 231 WP_011947173.1 exodeoxyribonuclease VII small subunit -
  LPP_RS11530 (lpp2278) - 2619664..2620560 (+) 897 WP_015961429.1 polyprenyl synthetase family protein -
  LPP_RS11535 (lpp2279) bioC 2620604..2621458 (-) 855 WP_015961430.1 malonyl-ACP O-methyltransferase BioC -
  LPP_RS11540 (lpp2280) comFC 2621502..2622206 (+) 705 WP_015961431.1 ComF family protein Machinery gene
  LPP_RS11545 (lpp2281) - 2622235..2623308 (-) 1074 WP_014844482.1 M50 family metallopeptidase -
  LPP_RS11550 (lpp2282) - 2623365..2623643 (-) 279 WP_010948040.1 hypothetical protein -
  LPP_RS17120 - 2623682..2623837 (+) 156 WP_162096598.1 hypothetical protein -
  LPP_RS11555 (lpp2283) hemA 2623961..2625235 (+) 1275 WP_015444133.1 glutamyl-tRNA reductase -
  LPP_RS11560 (lpp2284) prfA 2625219..2626307 (+) 1089 WP_010948042.1 peptide chain release factor 1 -
  LPP_RS11565 (lpp2285) prmC 2626300..2627163 (+) 864 WP_014844485.1 peptide chain release factor N(5)-glutamine methyltransferase -

Sequence

Protein


Download         Length: 234 a.a.        Molecular weight: 26744.34 Da        Isoelectric Point: 9.2792

>NTDB_id=1270 LPP_RS11540 WP_015961431.1 2621502..2622206(+) (comFC) [Legionella pneumophila str. Paris]
MLQKFLSITQNLRLPSICTLCNQFHKSQLAVCSNCMEFMKQLGSCCQYCAYPLSDDTYLVCGQCVRKRPSFDSAYIAYRF
EEPLRSLIHQFKYHNGLYLASFLKQLLLNALPKSALKPDCLIPVPMHPKRLKRRGFNQAAVLTRLLARQLNIPYDLYYCQ
KIINTASQANLDGEQRRKNLRHAFYVPPVTYEHVMIVDDLLTTGSTANEIAHTLKNAGVKRVDICCCARAVTKN

Nucleotide


Download         Length: 705 bp        

>NTDB_id=1270 LPP_RS11540 WP_015961431.1 2621502..2622206(+) (comFC) [Legionella pneumophila str. Paris]
GTGCTCCAAAAATTCTTAAGTATAACACAGAACTTACGTTTACCTTCAATATGCACTCTTTGCAACCAGTTCCACAAAAG
CCAACTTGCAGTTTGCTCTAATTGCATGGAGTTTATGAAACAACTTGGATCTTGCTGTCAGTACTGTGCCTACCCCCTTT
CTGATGATACGTATCTGGTTTGTGGTCAATGTGTTAGAAAAAGGCCCTCCTTTGATAGTGCTTATATAGCGTATCGTTTT
GAAGAGCCTCTGCGTAGTTTAATACATCAGTTTAAATACCATAATGGCCTTTATCTGGCCTCTTTTCTAAAACAATTACT
TCTTAATGCCTTACCCAAAAGCGCATTGAAACCAGATTGTCTAATTCCCGTTCCCATGCATCCCAAACGATTAAAACGAA
GGGGATTTAACCAGGCTGCTGTATTAACCAGATTATTAGCTCGTCAATTAAACATCCCTTATGATTTATATTATTGCCAA
AAAATAATCAATACCGCTTCACAAGCCAACCTTGATGGAGAGCAACGAAGAAAAAATTTACGTCATGCTTTTTATGTACC
ACCTGTAACTTATGAACACGTCATGATTGTTGATGACTTATTAACCACAGGCAGCACAGCCAATGAAATAGCACATACAT
TAAAAAATGCCGGGGTTAAGAGAGTAGATATATGTTGTTGTGCGCGGGCTGTAACTAAGAATTAA

Domains

Predicted by InterproScan.

Phosphoribosyl transferase domain (Pribosyltran)

(140-229)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFC Legionella pneumophila strain ERS1305867

100

100

1

Multiple sequence alignment    



References

[1] Léo Hardy et al. (2021) Transposon Insertion Sequencing in a Clinical Isolate of Legionella pneumophila Identifies Essential Genes and Determinants of Natural Transformation. Journal of Bacteriology 203(3):e00548-20. [PMID: 33168636]