Detailed information    

experimental Experimentally validated

Overview

Name   comF   Type   Unclear
Locus tag   HI_0434 Genome accession   L42023
Coordinates   456269..456958 (-) Length   229 a.a.
NCBI ID   AAC22093.1    Uniprot ID   -
Organism   Haemophilus influenzae Rd KW20     
Function   require for natural transformation   
Unclear

Function

Two mutations, rec2 and comF, were not transformable despite taking up near-normal amounts of DNA.


Genomic Context

Location: 451269..461958
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HI_0429 - 451754..453586 (-) 1833 AAC22088.1 glucosamine--fructose-6-phosphate aminotransferase (glmS) -
  HI_0430 - 453698..454108 (-) 411 AAC22089.1 DNA-binding protein HU-alpha (hupA) -
  HI_0431 - 454110..454700 (-) 591 AAC22090.1 conserved hypothetical protein -
  HI_0432 - 454736..455530 (-) 795 AAC22091.1 conserved hypothetical protein -
  HI_0433 - 455597..456193 (-) 597 AAC22092.1 orfG protein -
  HI_0434 comF 456269..456958 (-) 690 AAC22093.1 competence protein F (comF) Unclear
  HI_0435 comE 456967..458304 (-) 1338 AAC22094.1 competence protein E (comE) Machinery gene
  HI_0436 comD 458314..458727 (-) 414 AAC22095.1 competence protein D (comD) Machinery gene
  HI_0437 comC 458724..459245 (-) 522 AAC22096.1 competence protein C (comC) Machinery gene
  HI_0438 comB 459242..459748 (-) 507 AAC22097.1 competence protein B (comB) Machinery gene
  HI_0439 comA 459749..460546 (-) 798 AAC22098.1 competence protein A (comA) Machinery gene

Sequence

Protein


Download         Length: 229 a.a.        Molecular weight: 26791.17 Da        Isoelectric Point: 10.2903

>NTDB_id=1348 HI_0434 AAC22093.1 456269..456958(-) (comF) [Haemophilus influenzae Rd KW20]
MMNFFNFRCIHCRGNLHIAKNGLCSGCQKQIKSFPYCGHCGSELQYYAQHCGNCLKQEPSWDKMVIIGHYIEPLSILIQR
FKFQNQFWIDRTLARLLYLAVRDAKRTHQLKLPEAIIPVPLYHFRQWRRGYNQADLLSQQLSRWLDIPNLNNIVKRVKHT
YTQRGLSAKDRRQNLKNAFSLAVSKNEFPYRRVALVDDVITTGSTLNEISKLLRKLGVEEIQVWGLARA

Nucleotide


Download         Length: 690 bp        

>NTDB_id=1348 HI_0434 AAC22093.1 456269..456958(-) (comF) [Haemophilus influenzae Rd KW20]
ATGATGAATTTTTTTAATTTTCGCTGTATCCACTGTCGTGGCAATCTTCATATCGCAAAAAATGGGTTATGTTCAGGTTG
CCAAAAACAAATTAAATCTTTTCCTTATTGCGGTCATTGTGGTTCGGAATTGCAATATTATGCGCAGCATTGTGGGAATT
GTCTTAAACAAGAACCAAGTTGGGATAAGATGGTCATTATTGGGCATTATATTGAACCTCTTTCGATATTGATTCAGCGT
TTTAAATTTCAAAATCAATTTTGGATTGACCGCACTTTAGCTCGGCTTTTATATCTTGCGGTACGTGATGCTAAACGAAC
GCATCAACTTAAATTGCCAGAGGCAATCATTCCAGTGCCTTTATATCATTTTCGTCAGTGGCGACGGGGTTATAATCAGG
CAGATTTATTATCTCAGCAATTAAGTCGTTGGCTGGATATTCCTAATTTGAACAATATCGTAAAGCGTGTGAAACACACC
TATACTCAACGTGGTTTGAGTGCAAAAGATCGTCGTCAGAATTTAAAAAATGCCTTTTCTCTTGCTGTTTCGAAAAATGA
ATTTCCTTATCGTCGTGTTGCGTTGGTGGATGATGTGATTACTACTGGTTCTACACTCAATGAAATCTCAAAATTGTTGC
GAAAATTAGGTGTGGAGGAGATTCAAGTGTGGGGGCTGGCACGAGCTTAA

Domains

Predicted by InterproScan.

Phosphoribosyl transferase domain (Pribosyltran)

(183-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Haemophilus influenzae 86-028NP

97.807

100

0.978
  comFC Legionella pneumophila strain ERS1305867

36.598

98.98

0.362

Multiple sequence alignment    



References

[1] Sunita Sinha et al. (2012) Seventeen Sxy-dependent cyclic AMP receptor protein site-regulated genes are needed for natural transformation in Haemophilus influenzae. Journal of Bacteriology 194(19):5245-54. [PMID: 22821979]
[2] Brian A Dougherty et al. (1999) Identification of Haemophilus influenzae Rd transformation genes using cassette mutagenesis. Microbiology (Reading, England) 145 ( Pt 2):401-409. [PMID: 10075422]