Detailed information NTG0001369

Experimentally validated

Overview

• Name: comA
• Type: Machinery gene
• Locus tag: NTHI_RS02665
• Genome accession: NC_007146
• Coordinates: 526370..527167 (-)
• Length: 265 a.a.
• NCBI ID: WP_011272050.1
• Uniprot ID: -
• Organism: Haemophilus influenzae 86-028NP
• Function: type IV pilus biogenesis and function DNA binding and uptake

Function

The pilABCD and comABCDEF gene clusters are operons and that the product of each gene is essential for normal pilus function. Mutants with nonpolar deletions in each of the 10 pil and com genes had an adherence defect when primary human airway cells were used as the target. These mutants were also diminished in their ability to form a biofilm in vitro and, additionally, were deficient in natural transformation.

Genomic Context

Location: 521370..532167

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NTHI_RS02635 (NTHI0558)	nfuA	522217..522813 (-)	597	WP_005649300.1	Fe-S biogenesis protein NfuA	-
NTHI_RS02640 (NTHI0559)	comF	522889..523575 (-)	687	WP_011272045.1	ComF family protein	Unclear
NTHI_RS02645 (NTHI0560)	comE	523588..524925 (-)	1338	WP_011272046.1	type IV pilus secretin PilQ	Machinery gene
NTHI_RS02650 (NTHI0562)	comD	524935..525348 (-)	414	WP_011272047.1	pilus assembly protein PilP	Machinery gene
NTHI_RS02655 (NTHI0563)	comC	525345..525866 (-)	522	WP_011272048.1	competence protein ComC	Machinery gene
NTHI_RS02660 (NTHI0564)	comB	525863..526369 (-)	507	WP_011272049.1	competence protein ComB	Machinery gene
NTHI_RS02665 (NTHI0566)	comA	526370..527167 (-)	798	WP_011272050.1	pilus assembly protein PilM	Machinery gene
NTHI_RS02670 (NTHI0567)	-	527266..529860 (+)	2595	WP_011272051.1	penicillin-binding protein 1A	-
NTHI_RS02675 (NTHI0568)	-	529933..530778 (+)	846	WP_011272052.1	23S rRNA (adenine(2030)-N(6))-methyltransferase RlmJ	-
NTHI_RS02680 (NTHI0569)	-	530947..531276 (+)	330	WP_005629464.1	YbaB/EbfC family nucleoid-associated protein	-
NTHI_RS02685 (NTHI0570)	recR	531407..532009 (+)	603	WP_005626527.1	recombination mediator RecR	-

Sequence

Protein

• Length: 265 a.a.
• Molecular weight: 31392.66 Da
• Isoelectric Point: 6.2334

>NTDB_id=1369 NTHI_RS02665 WP_011272050.1 526370..527167(-) (comA) [Haemophilus influenzae 86-028NP]
MQFSLKNYRTLQIGIHRKQGYFDFVWFDDLEQPQSYQIFVNDRDFKNRFLQQLKTQYQGKTFPLQFVASIPAHLTWSKVL
MLPQVLNAQECHQQCKFVIEKELPISLNELWFDYRSTSLKQGFRLDVTAIRKSTAQTYLQDFQPFKINVLDVASNAILRA
FQYLLNEQVRSENTLFLFQEDDYCLAICERSQQSQILQSHENLTALYEQFTERFEGQLEQVFVYQIPSSHTPLPENWQRV
ETELPFIALGNALWQKDLHQQKVGG

Nucleotide

• Length: 798 bp

>NTDB_id=1369 NTHI_RS02665 WP_011272050.1 526370..527167(-) (comA) [Haemophilus influenzae 86-028NP]
ATGCAATTCTCCCTGAAAAATTACCGCACTTTACAAATCGGCATTCATCGTAAGCAGGGTTATTTTGATTTTGTGTGGTT
TGATGATCTTGAACAGCCACAAAGTTATCAAATTTTTGTTAATGATCGTGATTTTAAAAATCGTTTTTTACAACAGCTAA
AAACACAATATCAAGGGAAAACCTTTCCTTTACAGTTTGTGGCAAGCATTCCCGCTCACTTAACTTGGTCGAAAGTATTA
ATGTTGCCACAAGTGTTAAATGCGCAAGAATGTCATCAACAATGTAAATTTGTGATTGAAAAAGAGCTGCCTATTTCTTT
AAATGAATTATGGTTTGATTATCGTTCTACCTCGTTAAAGCAAGGTTTTCGATTAGACGTTACTGCAATTCGTAAAAGTA
CTGCTCAAACTTATTTGCAAGATTTTCAGCCATTTAAAATTAATGTATTGGATGTTGCGTCAAATGCTATTTTGCGTGCA
TTTCAGTATTTGTTGAATGAACAAGTGCGGTCAGAAAATACCTTATTTTTATTTCAAGAAGATGACTATTGTTTGGCGAT
CTGTGAAAGATCGCAGCAATCGCAAATTTTACAATCTCACGAAAATTTGACCGCACTTTATGAACAATTTACCGAACGTT
TTGAAGGACAACTTGAACAAGTTTTTGTTTATCAAATTCCCTCAAGTCATACACCATTACCCGAAAACTGGCAGCGAGTA
GAAACAGAACTCCCTTTTATTGCGCTTGGCAACGCGCTATGGCAAAAAGATTTACATCAACAAAAAGTGGGTGGTTAA

Domains

No domain identified.

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comA	Haemophilus influenzae Rd KW20	96.226	100	0.962

References

• [1]: Michael D Carruthers et al. (2012) Biological roles of nontypeable Haemophilus influenzae type IV pilus proteins encoded by the pil and com operons. Journal of Bacteriology 194(8):1927-33. [PMID: 22328674]