Detailed information NTG0001408

Experimentally validated

Overview

• Name: pilQ
• Type: Machinery gene
• Locus tag: DSB67_RS13980
• Genome accession: NZ_CP030788
• Coordinates: 2893213..2894955 (-)
• Length: 580 a.a.
• NCBI ID: WP_010643500.1
• Uniprot ID: -
• Organism: Vibrio campbellii strain DS40M4
• Function: type IV pilus biogenesis and function DNA binding and uptake

Function

Induction of TfoX expression resulted in upregulation of the genes required for DNA uptake and integration (comEC, comEA, comM, comF, and dprA) and pilus structure (pilABCD, pilMNOPQ, and minor pilins VC0857 to -0861) in DS40M4

Genomic Context

Location: 2888213..2899955

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DSB67_RS13960 (DSB67_13965)	-	2888918..2889757 (-)	840	WP_010643506.1	Dam family site-specific DNA-(adenine-N6)-methyltransferase	-
DSB67_RS13965 (DSB67_13970)	-	2889827..2891320 (-)	1494	WP_010643504.1	SPOR domain-containing protein	-
DSB67_RS13970 (DSB67_13975)	aroB	2891374..2892474 (-)	1101	WP_010643502.1	3-dehydroquinate synthase	-
DSB67_RS13975 (DSB67_13980)	aroK	2892506..2893024 (-)	519	WP_010443904.1	shikimate kinase AroK	-
DSB67_RS13980 (DSB67_13985)	pilQ	2893213..2894955 (-)	1743	WP_010643500.1	type IV pilus secretin PilQ	Machinery gene
DSB67_RS13985 (DSB67_13990)	pilP	2894990..2895505 (-)	516	WP_010643498.1	pilus assembly protein PilP	Machinery gene
DSB67_RS13990 (DSB67_13995)	pilO	2895516..2896088 (-)	573	WP_010643497.1	type 4a pilus biogenesis protein PilO	Machinery gene
DSB67_RS13995 (DSB67_14000)	pilN	2896081..2896656 (-)	576	WP_010643496.1	PilN domain-containing protein	Machinery gene
DSB67_RS14000 (DSB67_14005)	pilM	2896640..2897656 (-)	1017	WP_010643494.1	type IV pilus biogenesis protein PilM	Machinery gene

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
tfoX	pilQ	positive effect
tfoX	pilB	positive effect
tfoX	dprA	positive effect
tfoX	pilC	positive effect
tfoX	pilD	positive effect
tfoX	pilM	positive effect
tfoX	pilO	positive effect
tfoX	comM	positive effect
tfoX	pilA	positive effect
tfoX	comEC	positive effect
tfoX	comEA	positive effect
tfoX	pilP	positive effect
tfoX	comF	positive effect
tfoX	pilN	positive effect

Sequence

Protein

• Length: 580 a.a.
• Molecular weight: 63569.52 Da
• Isoelectric Point: 4.5343

>NTDB_id=1408 DSB67_RS13980 WP_010643500.1 2893213..2894955(-) (pilQ) [Vibrio campbellii strain DS40M4]
MKMRHGLTLTPRKLTGLFSLWFSLLLAPFVAAEEVQSNSLKSIDFRTNKDKDAVIVVELVSPAAIVDIKRVQEGLSIDLL
NTSVKDDQLYLLDVKDFSTVVESVEVFRDTSTTRLVAYIDEDYSHDYRLTGRYLEVKVGKLKANEKAPDKSILDKEGKLI
SINFQDIPVRNVLQLIADYNQFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKDELDLREKQQ
LEKQKLEKEIGELSSEIIKVNFAKASDIAEMINGEGNISMLSDRGSMTIDERTNSLLIRELPENIEVIREIIESLDIPVK
QVQIEARIVTINEGNMDELGVRWGFTSINGSHTVGGSIENNLATIGLYDGGGEGDDGGSSVGIDDFLNVNLAATSPNATS
IAFQVAKLGSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVTFKKAVLSLKVTPQIT
PDNRLVLDLSVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPLLGALFRRS
YENVGKSELLIFVTPKVVIQ

Nucleotide

• Length: 1743 bp

>NTDB_id=1408 DSB67_RS13980 WP_010643500.1 2893213..2894955(-) (pilQ) [Vibrio campbellii strain DS40M4]
ATGAAAATGAGACACGGATTAACACTGACACCAAGGAAGCTAACAGGGCTCTTCTCTTTATGGTTCTCTCTACTTTTAGC
CCCTTTCGTCGCAGCAGAAGAAGTACAGTCTAACTCGCTTAAGAGCATCGACTTCCGTACGAACAAAGACAAAGACGCCG
TGATTGTGGTTGAGCTCGTATCACCCGCTGCGATTGTTGATATTAAACGCGTACAAGAAGGCTTGAGTATCGACTTACTC
AACACCTCCGTTAAAGATGACCAGCTTTACCTACTTGATGTGAAAGATTTCTCGACAGTTGTCGAGAGCGTTGAGGTATT
CCGCGATACTTCTACAACACGTCTGGTGGCTTATATCGATGAGGACTATTCTCACGACTACCGTCTTACTGGTCGTTATT
TAGAGGTAAAGGTTGGCAAGCTTAAAGCCAACGAAAAAGCGCCGGATAAAAGCATTTTGGACAAAGAAGGCAAGCTAATT
TCGATTAACTTCCAAGACATTCCTGTGCGAAACGTGTTGCAACTTATCGCCGACTACAATCAATTTAACTTGGTCGTCTC
TGACTCGGTCGAGGGTAACTTGACCTTGCGTTTGGATGGTGTGCCTTGGCAGCAAGTACTTGACATTATCTTGCAAGTGA
AAGGGTTAGATAAGCGTGTTGATGGTAATGTCATTCTCGTCGCACCAAAAGATGAATTGGATCTGCGCGAGAAGCAGCAA
CTCGAAAAGCAAAAGCTAGAGAAAGAGATCGGTGAGCTTTCATCAGAGATCATCAAGGTGAACTTCGCTAAAGCGTCAGA
CATTGCTGAGATGATCAATGGAGAGGGCAACATCAGTATGCTTTCAGATCGAGGCAGCATGACCATTGATGAGCGTACCA
ACTCGTTATTGATTCGTGAGTTACCTGAGAATATTGAAGTGATTCGCGAGATCATAGAATCATTGGATATTCCTGTAAAA
CAGGTTCAGATTGAAGCACGTATTGTGACTATTAATGAAGGTAACATGGACGAACTTGGCGTTCGTTGGGGCTTCACTTC
CATTAATGGCAGTCATACTGTGGGTGGTTCAATTGAGAATAACCTTGCGACAATCGGCCTGTACGATGGCGGCGGTGAAG
GTGATGACGGTGGCAGCAGTGTGGGTATTGATGACTTTCTCAACGTTAACCTTGCCGCGACCAGTCCAAATGCGACCAGT
ATAGCCTTTCAAGTTGCGAAGTTAGGTTCGGACACCTTACTAGATTTGGAACTTTCGGCGCTGCAACAAGAATCGAAAGC
AGAAATTATTTCTAGCCCACGTTTGATAACCACGAATAAGAAGCCAGCTTACATTGAGCAGGGTACCGAGATCCCTTACC
TTGAATCTTCTTCCAGTGGTGCGACTTCTGTGACGTTCAAAAAAGCGGTATTGAGCTTGAAAGTGACGCCACAGATTACG
CCTGACAACCGCTTGGTGTTGGATTTGAGCGTTACGCAAGACAGACCGGGCCAAGTGGTAAAAACAGGCACTGGTGAGGC
GGTGGCGATAGATACGCAACGTATTGGTACACAAGTTTTAGTTAATAATGGCGAGACAGTCGTGTTGGGCGGTATTTTTC
AACACAGCATTACAAACTCCGTCGATAAGGTGCCTTTATTAGGTGATTTGCCGCTGTTAGGTGCGCTTTTCCGTCGAAGT
TACGAAAATGTCGGTAAAAGTGAACTACTGATTTTTGTTACACCTAAAGTTGTGATTCAGTAA

Domains

Predicted by InterproScan.

• Bacterial type II and III secretion system protein (Secretin) (420-578)

• Bacterial type II/III secretion system short domain (Secretin_N) (256-321)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilQ	Vibrio cholerae strain A1552	78.545	96.322	0.757
pilQ	Vibrio cholerae O1 biovar El Tor strain E7946	78.545	96.322	0.757
comE	Haemophilus influenzae 86-028NP	42.959	94.157	0.404
comE	Haemophilus influenzae Rd KW20	42.482	94.157	0.4
pilQ	Legionella pneumophila strain ERS1305867	41.894	94.655	0.397
pilQ	Legionella pneumophila strain Lp02	41.894	94.655	0.397
pilQ	Pseudomonas aeruginosa PAK	40.354	97.414	0.393
pilQ	Acinetobacter baumannii strain A118	37.521	100	0.386
pilQ	Acinetobacter baumannii D1279779	37.521	100	0.386
comE	Glaesserella parasuis strain SC1401	36.967	100	0.371
comQ	Acinetobacter baylyi ADP1	36.788	99.828	0.367

References

• [1]: Chelsea A Simpson et al. (2019) Diversity in Natural Transformation Frequencies and Regulation across Vibrio Species. MBio 10(6):e02788-19. [PMID: 31848285]