Detailed information NTG0001400

Experimentally validated

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: DSB67_RS12660
• Genome accession: NZ_CP030788
• Coordinates: 2640576..2641040 (+)
• Length: 154 a.a.
• NCBI ID: WP_010643261.1
• Uniprot ID: -
• Organism: Vibrio campbellii strain DS40M4
• Function: type IV pilus biogenesis and function DNA binding and uptake

Function

Induction of TfoX expression resulted in upregulation of the genes required for DNA uptake and integration (comEC, comEA, comM, comF, and dprA) and pilus structure (pilABCD, pilMNOPQ, and minor pilins VC0857 to -0861) in DS40M4

Genomic Context

Location: 2635576..2646040

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DSB67_RS12640 (DSB67_12645)	pdhR	2637608..2638375 (-)	768	WP_005428674.1	pyruvate dehydrogenase complex transcriptional repressor PdhR	-
DSB67_RS12645 (DSB67_12650)	ampD	2638780..2639331 (-)	552	WP_010643266.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
DSB67_RS12650 (DSB67_12655)	nadC	2639424..2640311 (+)	888	WP_010643264.1	carboxylating nicotinate-nucleotide diphosphorylase	-
DSB67_RS12660 (DSB67_12665)	pilA	2640576..2641040 (+)	465	WP_010643261.1	pilin	Machinery gene
DSB67_RS12665 (DSB67_12670)	pilB	2641040..2642725 (+)	1686	WP_010643259.1	type IV-A pilus assembly ATPase PilB	Machinery gene
DSB67_RS12670 (DSB67_12675)	pilC	2642760..2643983 (+)	1224	WP_010643257.1	type II secretion system F family protein	Machinery gene
DSB67_RS12675 (DSB67_12680)	pilD	2644056..2644925 (+)	870	WP_010643256.1	prepilin peptidase	Machinery gene
DSB67_RS12680 (DSB67_12685)	coaE	2644926..2645540 (+)	615	WP_010643253.1	dephospho-CoA kinase	-

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
tfoX	pilA	positive effect
tfoX	pilB	positive effect
tfoX	dprA	positive effect
tfoX	pilC	positive effect
tfoX	pilD	positive effect
tfoX	pilM	positive effect
tfoX	pilO	positive effect
tfoX	comM	positive effect
tfoX	pilQ	positive effect
tfoX	comEC	positive effect
tfoX	comEA	positive effect
tfoX	pilP	positive effect
tfoX	comF	positive effect
tfoX	pilN	positive effect

Sequence

Protein

• Length: 154 a.a.
• Molecular weight: 16193.61 Da
• Isoelectric Point: 8.8069

>NTDB_id=1400 DSB67_RS12660 WP_010643261.1 2640576..2641040(+) (pilA) [Vibrio campbellii strain DS40M4]
MKTNKQKKQQGFTLIELMIVVAIIGILAALAIPAYQDYTKKATMAEFPKIASSTKLAVELCAHEHAADAGAFKTKCHQTA
NGIPAAFTLNNLTVTPAAGAASGAISITVNAANDKGPIKQNEKYILTATYASNGVTWSSECKDATNAVQTSYCP

Nucleotide

• Length: 465 bp

>NTDB_id=1400 DSB67_RS12660 WP_010643261.1 2640576..2641040(+) (pilA) [Vibrio campbellii strain DS40M4]
ATGAAAACGAATAAACAGAAGAAGCAGCAAGGTTTTACGCTGATTGAATTGATGATTGTGGTAGCGATTATTGGTATTTT
GGCAGCATTGGCAATCCCGGCTTATCAAGACTACACGAAGAAAGCAACAATGGCAGAGTTCCCTAAAATTGCATCTTCTA
CAAAACTAGCAGTTGAGCTTTGTGCTCATGAACACGCAGCAGATGCTGGGGCATTTAAGACTAAATGTCACCAAACGGCG
AATGGGATACCTGCTGCCTTCACCCTAAATAATCTAACAGTAACGCCAGCAGCTGGGGCAGCTTCGGGTGCCATTAGTAT
TACGGTAAATGCAGCAAACGACAAAGGTCCAATTAAGCAAAACGAAAAATACATTTTAACTGCAACGTATGCTTCCAATG
GAGTTACATGGTCCTCAGAATGTAAAGATGCGACCAACGCAGTACAAACCTCTTACTGCCCATAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (7-31)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA2	Legionella pneumophila strain ERS1305867	49.242	85.714	0.422
pilA2	Legionella pneumophila str. Paris	48.485	85.714	0.416
pilA/pilA1	Eikenella corrodens VA1	38.125	100	0.396
comP	Acinetobacter baylyi ADP1	41.912	92.517	0.388
pilA	Haemophilus influenzae 86-028NP	41.844	91.558	0.383
pilA	Haemophilus influenzae Rd KW20	39.716	94.631	0.376

References

• [1]: Chelsea A Simpson et al. (2019) Diversity in Natural Transformation Frequencies and Regulation across Vibrio Species. MBio 10(6):e02788-19. [PMID: 31848285]