Detailed information    

experimental Experimentally validated

Overview

Name   pilO   Type   Machinery gene
Locus tag   DSB67_RS13990 Genome accession   NZ_CP030788
Coordinates   2895516..2896088 (-) Length   190 a.a.
NCBI ID   WP_010643497.1    Uniprot ID   -
Organism   Vibrio campbellii strain DS40M4     
Function   type IV pilus biogenesis and function   
DNA binding and uptake

Function

Induction of TfoX expression resulted in upregulation of the genes required for DNA uptake and integration (comEC, comEA, comM, comF, and dprA) and pilus structure (pilABCD, pilMNOPQ, and minor pilins VC0857 to -0861) in DS40M4


Genomic Context

Location: 2890516..2901088
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DSB67_RS13970 (DSB67_13975) aroB 2891374..2892474 (-) 1101 WP_010643502.1 3-dehydroquinate synthase -
  DSB67_RS13975 (DSB67_13980) aroK 2892506..2893024 (-) 519 WP_010443904.1 shikimate kinase AroK -
  DSB67_RS13980 (DSB67_13985) pilQ 2893213..2894955 (-) 1743 WP_010643500.1 type IV pilus secretin PilQ Machinery gene
  DSB67_RS13985 (DSB67_13990) pilP 2894990..2895505 (-) 516 WP_010643498.1 pilus assembly protein PilP Machinery gene
  DSB67_RS13990 (DSB67_13995) pilO 2895516..2896088 (-) 573 WP_010643497.1 type 4a pilus biogenesis protein PilO Machinery gene
  DSB67_RS13995 (DSB67_14000) pilN 2896081..2896656 (-) 576 WP_010643496.1 PilN domain-containing protein Machinery gene
  DSB67_RS14000 (DSB67_14005) pilM 2896640..2897656 (-) 1017 WP_010643494.1 type IV pilus biogenesis protein PilM Machinery gene
  DSB67_RS14005 (DSB67_14010) - 2897835..2900402 (+) 2568 WP_010643492.1 penicillin-binding protein 1A -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  tfoX pilO positive effect
  tfoX pilB positive effect
  tfoX dprA positive effect
  tfoX pilC positive effect
  tfoX pilD positive effect
  tfoX pilM positive effect
  tfoX comM positive effect
  tfoX pilA positive effect
  tfoX pilQ positive effect
  tfoX comEC positive effect
  tfoX comEA positive effect
  tfoX pilP positive effect
  tfoX comF positive effect
  tfoX pilN positive effect

Sequence

Protein


Download         Length: 190 a.a.        Molecular weight: 22182.58 Da        Isoelectric Point: 4.8928

>NTDB_id=1406 DSB67_RS13990 WP_010643497.1 2895516..2896088(-) (pilO) [Vibrio campbellii strain DS40M4]
MVNWQDLEMDEIAEWPLVPQLVAILLMVVLIQGFGYWFYLKPEEARLNTLIHEEQTLKVALRIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGDKESEHFLYRLPLNIELTGDFHDIGRFSQAIATLPRII
TFQDVTWQRVSQESETLHFRVRANTYQFKP

Nucleotide


Download         Length: 573 bp        

>NTDB_id=1406 DSB67_RS13990 WP_010643497.1 2895516..2896088(-) (pilO) [Vibrio campbellii strain DS40M4]
ATGGTTAATTGGCAAGACCTAGAAATGGATGAAATCGCTGAATGGCCGCTGGTACCTCAGTTGGTGGCGATTCTTCTTAT
GGTGGTGTTGATCCAAGGTTTTGGTTACTGGTTTTATTTGAAGCCAGAGGAAGCTCGCCTTAATACCTTGATCCATGAAG
AGCAGACTTTAAAAGTCGCGCTGAGGATCAAAGCCAATAAAGTAGCTGCACTGCCGCAGCTTCAGTCACAACTTGATGAA
CTGACCACCCGCTATGAACTGCTTTTGCAACAACTGCCGGCACAGAAAGAATTGGCGACCATGCTAGCGTCGGTTAATGA
ACTCGGCATCGATAACAAGCTGACATTTACGCGTATTGATTGGGGAGATAAAGAGTCAGAGCACTTCCTTTACCGTTTGC
CGCTCAATATTGAGCTAACGGGTGACTTCCATGACATCGGTCGTTTTTCACAGGCCATCGCCACCTTACCACGCATCATC
ACATTTCAAGATGTAACTTGGCAGCGAGTCAGTCAAGAGAGTGAAACCTTGCACTTCCGTGTAAGAGCAAACACTTACCA
ATTCAAACCATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(48-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

71.958

99.474

0.716

Multiple sequence alignment    



References

[1] Chelsea A Simpson et al. (2019) Diversity in Natural Transformation Frequencies and Regulation across Vibrio Species. MBio 10(6):e02788-19. [PMID: 31848285]