Detailed information NTG0001405

Experimentally validated

Overview

• Name: pilN
• Type: Machinery gene
• Locus tag: DSB67_RS13995
• Genome accession: NZ_CP030788
• Coordinates: 2896081..2896656 (-)
• Length: 191 a.a.
• NCBI ID: WP_010643496.1
• Uniprot ID: -
• Organism: Vibrio campbellii strain DS40M4
• Function: type IV pilus biogenesis and function DNA binding and uptake

Function

Induction of TfoX expression resulted in upregulation of the genes required for DNA uptake and integration (comEC, comEA, comM, comF, and dprA) and pilus structure (pilABCD, pilMNOPQ, and minor pilins VC0857 to -0861) in DS40M4

Genomic Context

Location: 2891081..2901656

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DSB67_RS13970 (DSB67_13975)	aroB	2891374..2892474 (-)	1101	WP_010643502.1	3-dehydroquinate synthase	-
DSB67_RS13975 (DSB67_13980)	aroK	2892506..2893024 (-)	519	WP_010443904.1	shikimate kinase AroK	-
DSB67_RS13980 (DSB67_13985)	pilQ	2893213..2894955 (-)	1743	WP_010643500.1	type IV pilus secretin PilQ	Machinery gene
DSB67_RS13985 (DSB67_13990)	pilP	2894990..2895505 (-)	516	WP_010643498.1	pilus assembly protein PilP	Machinery gene
DSB67_RS13990 (DSB67_13995)	pilO	2895516..2896088 (-)	573	WP_010643497.1	type 4a pilus biogenesis protein PilO	Machinery gene
DSB67_RS13995 (DSB67_14000)	pilN	2896081..2896656 (-)	576	WP_010643496.1	PilN domain-containing protein	Machinery gene
DSB67_RS14000 (DSB67_14005)	pilM	2896640..2897656 (-)	1017	WP_010643494.1	type IV pilus biogenesis protein PilM	Machinery gene
DSB67_RS14005 (DSB67_14010)	-	2897835..2900402 (+)	2568	WP_010643492.1	penicillin-binding protein 1A	-
DSB67_RS14010 (DSB67_14015)	oxyR	2900480..2901388 (-)	909	WP_010643491.1	DNA-binding transcriptional regulator OxyR	-

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
tfoX	pilN	positive effect
tfoX	pilB	positive effect
tfoX	dprA	positive effect
tfoX	pilC	positive effect
tfoX	pilD	positive effect
tfoX	pilM	positive effect
tfoX	pilO	positive effect
tfoX	comM	positive effect
tfoX	pilA	positive effect
tfoX	pilQ	positive effect
tfoX	comEC	positive effect
tfoX	comEA	positive effect
tfoX	pilP	positive effect
tfoX	comF	positive effect

Sequence

Protein

• Length: 191 a.a.
• Molecular weight: 22167.46 Da
• Isoelectric Point: 9.1159

>NTDB_id=1405 DSB67_RS13995 WP_010643496.1 2896081..2896656(-) (pilN) [Vibrio campbellii strain DS40M4]
MLYHINLLAWRENQREEHRRRFIGLILLGAIAALGVQWGIGKYYQYQQDQQQQRLDYLAHYISQLDTRIEAMKIAEQEHS
KILERLKTVEGLQNGRNKTTEFMNLMPSVIPEGVYVDKIKMNDYEIEISGISDSTSRLATMLDNMERSAKLLDVDMHSIV
HGKARFGKEFQTFKVSFMFKDASIKGGKQHG

Nucleotide

• Length: 576 bp

>NTDB_id=1405 DSB67_RS13995 WP_010643496.1 2896081..2896656(-) (pilN) [Vibrio campbellii strain DS40M4]
ATGCTGTATCACATTAATCTTCTTGCCTGGCGTGAAAACCAACGTGAAGAGCATCGTCGTCGTTTTATTGGCTTGATTTT
ACTCGGGGCGATCGCTGCTCTGGGCGTGCAATGGGGGATCGGTAAGTACTACCAATACCAACAAGATCAGCAGCAACAGC
GCTTGGATTATCTTGCTCACTATATCTCTCAACTCGATACACGTATTGAAGCCATGAAGATAGCAGAGCAAGAGCACAGC
AAAATTCTAGAGCGCTTAAAGACCGTTGAGGGGCTGCAAAATGGGCGCAATAAAACCACTGAGTTTATGAACCTGATGCC
CTCAGTGATCCCTGAAGGCGTCTATGTCGATAAGATCAAAATGAACGACTACGAGATTGAGATCTCGGGTATTTCTGATA
GCACCTCTCGTCTTGCCACCATGCTCGATAATATGGAGCGTTCTGCCAAGCTTCTGGATGTTGATATGCACTCCATTGTC
CATGGTAAAGCGCGCTTTGGTAAAGAGTTCCAGACGTTCAAAGTCTCCTTTATGTTTAAAGATGCCTCTATAAAAGGAGG
TAAGCAGCATGGTTAA

Domains

Predicted by InterproScan.

• Fimbrial assembly protein (PilN) (PilN) (103-176)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilN	Vibrio cholerae strain A1552	60.452	92.67	0.56

References

• [1]: Chelsea A Simpson et al. (2019) Diversity in Natural Transformation Frequencies and Regulation across Vibrio Species. MBio 10(6):e02788-19. [PMID: 31848285]